专利号:US-7423171-B2 优先权日:2006-06-20 标题 :One-step catalytic process for the synthesis of isocyanates 发明人:SERRANO FERNANDEZ FRANCISCO LU; ALMENA MUNOZ BEATRIZ; PADILLA POLO ANA; OREJON ALVAREZ ARANCHA; CLAVER CABRERO CARMEN; CASTILLON MIRANDA SERGIO; SALAGRE CARNERO PILAR; AGHMIZ ALI 权利人:REPSOL YPF SA 摘要:A one-pot process for the synthesis of isocyanates, polyisocyanates or mixtures thereof which includes the steps of:n i. preparing a mixture comprising an amine, an alcohol, an oxygen-containing gas, carbon monoxide, a metal complex catalyst selected from the group consisting of macrocyclic complex catalysts and cobalt Shiff base catalysts; and a solvent selected from the group consisting of aliphatic or aromatic halocarbons, perhalogenated alcohols, halogenated ethers, halogenated ketones, perfluorinated hydrocarbons, polymers of chlorotrifluoroethylene having the formula —(CF 2 —CFCl) n wherein n is between 2 and 10, and mixtures thereof; ii. subjecting the resulting mixture to a first heating under pressure; iii. cooling and depressurizing the mixture resulting from the previous step; and iv. subjecting the mixture of the previous step to a second heating to separate out the isocyanate product from the mixture.
专利号:WO-2024163733-A1 优先权日:2023-02-01 标题:Electrochemical synthesis with redox stable nucleotides 发明人:WU TIANDI; LACKEY JEREMY; LIN YANYOU; PITSCH STEFAN 权利人:TWIST BIOSCIENCE CORP 摘要:Provided herein are compositions, devices, systems and methods for constructing and storing polynucleotides encoding information with redox resistant bases. The compositions, devices, systems, and methods described herein provide for storage or synthesis of a library comprising a plurality of polynucleotides with one or more redox resistance bases. Further provided herein are methods to increase DNA synthesis yield and fidelity.
专利号:WO-9925385-A1 优先权日:1997-11-17 标 题:A method of increasing nucleic acid synthesis with ultrasound 发明人:UNGER EVAN C; MCCREERY THOMAS; SADEWASSER DAVID 权利人:IMARX PHARMACEUTICAL CORP 摘要:The present invention is directed to a method of increasing nucleic acid synthesis in a cell comprising administering to the cell a therapeutically effective amount of ultrasound for a therapeutically effective time such that said administration of said ultrasound results in said increased nucleic acid synthesis. The nucleic acid sequence may comprise an endogenous sequence or an exogenous sequence.
专利号:US-2004076964-A1 优先权日:2002-10-22 标 题:Methods for in situ generation of nucleic acid arrays 发明人:LEPROUST ERIC M; WOODWORTH ROBERT E 摘要:Methods of producing nucleic acid arrays using an in situ nucleic acid synthesis protocol are provided, where the in situ nucleic acid synthesis protocol includes a plurality of cycles, each of which includes: (I) a monomer attachment step; and (II) a functional group, e.g., 5′OH, generation step, the latter of which includes: (a) oxidation and (b) deblocking substeps. A feature of the subject methods is that the deblocking substep is performed by contacting a substrate surface with a deblocking solution made up of a deblocking agent present in an organic solvent, wherein the organic solvent is toluene or another organic solvent having substantially the same vapor pressure. Also provided are the arrays produced using the subject methods, as well as methods for use of the arrays and kits that include the same.
专利号:US-2005214778-A1 优先权日:2004-03-29 标题:Methods for in situ generation of nucleic acid arrays 发明人:PECK BILL J; LEPROUST ERIC M 权利人:PECK BILL J; LEPROUST ERIC M 摘要:Methods of producing nucleic acid arrays using an in situ nucleic acid synthesis protocol are provided, where the in situ nucleic acid synthesis protocol includes a plurality of cycles, each of which includes: (I) a monomer attachment step; and (II) a functional group generation step. A feature of the subject methods is that the functional group generation step is performed by sequentially flowing a plurality of different fluids across the surface of a substrate in a manner such that the surface is not contacted with a with a triple phase interface line. Also provided are the arrays produced using the subject methods, as well as methods for use of the arrays and kits that include the same.
专利号:US-2022323924-A1 优先权日:2021-03-24 标题:Electrochemical polynucleotide synthesis 发明人:LACKEY JEREMY; HABIBIAN MARYAM; LIN YANYOU 权利人:TWIST BIOSCIENCE CORP 摘要:Provided herein are compositions, devices, systems and methods for generation and use of biomolecule-based information for storage. Further provided are devices comprising addressable electrodes controlling polynucleotide synthesis (deprotection, extension, or cleavage, etc.) Further provided are compositions for low voltage deprotection of polynucleotides.
[参考文献]: Jian Li, Et Al. Pickering Emulsion Templated Interfacial Atom Transfer Radical Polymerization For Microencapsulation. Langmuir. 2010 Dec 7;26(23):17926-35. [参考文献]: Lorenzo Tattini, Et Al. Gels From A Semifluorinated N-Alkane In Fluorinated Solvents As A Probe For Intermolecular Interactions. J Colloid Interface Sci. 2009 Nov 1;339(1):259-65.
合成参考文献
摘要:Summary Tables of Biological Tests, National Research Council Chemical-Biological Coordination Center., 4(108), 1952 摘要:S25 | OECDPFAS | List of PFAS from the OECD | DOI:10.5281/zenodo.2648775