专利号:JP-6839666-B2 优先权日:2015-06-25 标 题:Methods, devices, and systems for the analysis of complex heterogeneous microbial strains, the prediction and identification of functional relationships and their interactions, and the selection and synthesis of microbial ensembles based on them.
专利号:US-2003186237-A1 优先权日:2001-02-14 标题:Methods and compositions of amplifying RNA 发明人:GINSBERG STEPHEN D; CHE SHAOLI 权利人:BAYLOR COLLEGE MEDICINE 摘要:The present invention pertains to a method that will increase the efficiency of second strand cDNA synthesis through a mechanism of “terminal continuationâ€? before further RNA amplification by RNA transcription using, for example, a bacteriophage promoter. In a specific embodiment, a transcription promoter is attached to the 5′ region of cDNA through the same mechanism of “terminal continuationâ€?. Genetic signals are subsequently amplified in a linear manner through RNA transcription. In specific embodiments, the orientation of the transcribed RNA is either sense or antisense, depending on the desired downstream application. In other embodiments, the present invention pertains to methods for extraction and amplification of RNA, particularly mRNA, from histologically stained tissues and cells.
专利号:CN-114958715-A 优先权日:2021-02-21 标题:A method for establishing a jujube cell suspension culture and using the method to regulate the synthesis of cyclic adenosine monophosphate
专利号:WO-2024081885-A1 优先权日:2022-10-13 标 题 :Method for inhibiting clostridioides difficile spore germination 发明人:QIAN YUANYUAN; CHANG MAYLAND; MOBASHERY SHAHRIAR 权利人:UNIV NOTRE DAME DU LAC 摘要:Oxadiazole antibiotics that exhibit bactericidal activity against C. difficile vegetative cells. We screened a library of 75 oxadiazoles against C. difficile ATCC 43255. The findings from this collection served as the basis for the syntheses of an additional 58 analogs, which were tested against the same strain. We discovered a potent (MIC 50 = 0.5 µg/mL, MIC 90 = 1 µg/mL for 101 C. difficile strains) and narrow-spectrum oxadiazole (3-(4-(cyclopentyloxy)phenyl)-5-(4-nitro-1 H -imidazol-2-yl)-1,2,4-oxadiazole; compound 57) that is not active against common gut bacteria or other tested organisms, but is selectively bactericidal against C. difficile and targets cell-wall synthesis. Other similarly effective oxadiazole antibiotics of formula I and II are described herein, several of which inhibit or prevent C. difficile spore germination.
专利号:CN-117586975-A 优先权日:2024-01-17 标 题:Application of dopa dioxygenase HhDODA1 and encoding gene thereof in beet pigment synthesis
专利号:CN-117586975-B 优先权日:2024-01-17 标 题:DOPA dioxygenase HhDODA1 and application of coding gene thereof in beet pigment synthesis
1: Rezano A, Ridhayanti F, Rangkuti AR, Gunawan T, Winarno GNA, Wijaya I. Cytotoxicity of Simvastatin in Human Breast Cancer MCF-7 and MDA-MB-231 Cell Lines. Asian Pac J Cancer Prev. 2021 Feb 1;22(S1):33-42. doi: 10.31557/APJCP.2021.22.S1.33. 22(3):1338. doi: 10.3390/ijms22031338. 11(2):460. doi: 10.3390/ani11020460. 26(4):845. doi: 10.3390/molecules26040845. 337:109337. doi: 10.1016/j.cbi.2020.109337. Epub ahead of print. 18(4):1380. doi: 10.3390/ijerph18041380. 120:111700. doi: 10.1016/j.msec.2020.111700. Epub 2020 Nov 6. 16(2):e0246516. doi: 10.1371/journal.pone.0246516. 11(2):323. doi: 10.3390/nano11020323.
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