CAS: 70-18-8; (S)-2-Amino-5-(((R)-1-((Carboxymethyl)Amino)-3-Mercapto-1-Oxopropan-2-yl)Amino)-5-Oxopentanoic Acid

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N8-Mono-Glutathionylspermidine置于trypanosoma Brucei Trypanothione Synthetase体系中,用 Aq. Buffer 作为反应溶剂,化学反应生成 谷胱甘肽
参考文献:Dissecting The Catalytic Mechanism Of Trypanosoma Brucei Trypanothione Synthetase By Kinetic Analysis And Computational Modeling
标题:Dissecting The Catalytic Mechanism Of Trypanosoma Brucei Trypanothione Synthetase By Kinetic Analysis And Computational Modeling
摘要:In Pathogenic Trypanosomes,Trypanothione Synthetase (Trys) Catalyzes The Synthesis Of Both Glutathionylspermidine (Gsp) And Trypanothione (Bis(Glutathionyl) Spermidine (T(Sh)(2))). Here We Present A Thorough Kinetic Analysis Of Trypanosoma Brucei Trys In A Newly Developed Phosphate Buffer System At Ph 7.0 And 37 Degrees C,Mimicking The Physiological Environment Of The Enzyme In The Cytosol Of Bloodstream Parasites. Under These Conditions,Trys Displays K-M Values For Gsh,Atp,Spermidine,And Gsp Of 34,18,687,And 32 Mu M,Respectively,As Well As K-I Values For Gsh And T(Sh) 2 Of 1 Mm And 360 Mu M,Respectively. As Gsp Hydrolysis Has A K-M Value Of 5.6 Mm,The In Vivo Amidase Activity Is Probably Negligible. To Obtain Deeper Insight In The Molecular Mechanism Of Trys,We Have Formulated Alternative Kinetic Models,With Elementary Reaction Steps Represented By Linear Kinetic Equations. The Model Parameters Were Fitted To The Extensive Matrix Of Steady-State Data Obtained For Different Substrate/product Combinations Under The In Vivo-Like Conditions. The Best Model Describes The Full Kinetic Profile And Is Able To Predict Time Course Data That Were Not Used For Fitting. This System'S Biology Approach To Enzyme Kinetics Led Us To Conclude That (I) Trys Follows A Ter-Reactant Mechanism,(II) The Intermediate Gsp Dissociates From The Enzyme Between The Two Catalytic Steps,And (Iii) T(Sh)(2) Inhibits The Enzyme By Remaining Bound At Its Product Site And,As Does The Inhibitory Gsh,By Binding To The Activated Enzyme Complex. The Newly Detected Concerted Substrate And Product Inhibition Suggests That Trys Activity Is Tightly Regulated.
DOI:10.1074/jbc.M113.483289

专利信息

专利号:US-7041479-B2
优先权日:2000-09-06
标题 :Enhanced in vitro synthesis of active proteins containing disulfide bonds
发明人：SWARTZ JAMES ROBERT; KIM DONG-MYUNG
权利人:TRUSTESS OF THE LELAND STANFOR
摘要:Compositions and methods are provided for the enhanced in vitro synthesis of polypeptides containing disulfide bonds. In order to improve the performance of in vitro protein synthesis reactions, pre-treatment and redox buffering of the reaction mix is performed in order to optimize the redox potential. Exogenous enzymes that enhance protein folding and disulfide bond formation may also be added to the reaction.

专利号:US-7456003-B2
优先权日:1994-05-18
标题:DNA sequences coding for enzymes capable of facilitating the synthesis of linear α-1,4 glucans in plants, fungi and microorganisms
发明人：KOSSMANN JENS; BUTTCHER VOLKER; WELSH THOMAS
权利人:BAYER BIOSCIENCE GMBH
摘要:DNA sequences coding for enzymes capable of facilitating the synthesis of linear â–¡-1,4 glucans in plants, fungi and microorganisms. The invention relates to DNA sequences Coding for proteins having the enzymatic activity of an amylosucrase which allows the synthesis of linear â–¡1,4 glucans from the substrate sucrose by bacteria, fungi and plants or in cell-free systems. The invention furthermore describes plasmids and bacteria containing these DNA sequences as well as processes for the production of plants and microorganisms capable of intracellularly or extracellularly expressing a polypeptide having amylosucrase activity. The invention furthermore relates to the production of pure fructose using proteins exhibiting the enzymatic activity of amylosucrase.

专利号:US-11059849-B2
优先权日:2014-09-02
标题 :Modified nucleotides for synthesis of nucleic acids, a kit containing such nucleotides and their use for the production of synthetic nucleic acid sequences or genes
发明人：YBERT THOMAS; GARIEL SYLVAIN
权利人:DNA SCRIPT
摘要:A modified nucleotide, intended for the synthesis of long chain nucleic acids by enzymatic processes, comprising a “naturalâ€? nitrogenous base or a natural nitrogenous base analogue, a ribose or deoxyribose carbohydrate, and at least one phosphate group, characterized in that said nucleotide comprises at least one R group, termed the modifier group, carried by said nitrogenous base or analogue and/or by the oxygen in position 3′ of the ribose or deoxyribose molecule, making it possible to block the polymerization of said nucleotide and/or to allow the interaction of said nucleotide with another molecule, such as a protein, during the nucleic acid synthesis, R comprising at least one functional terminal group.

专利号:US-10695450-B2
优先权日:2016-07-26
标 题 :Synthesis of a radioactive agent composition
发明人：VIOT GILLES
权利人:LABORATOIRES CYCLOPHARMA
摘要:The present invention relates to a method for the synthesis of a radioactive agent composition comprising at least a purification step carried out in the presence of an antioxidant, to the composition obtained by this method comprising radioactive agent and excipient, and to the method for preventing radiolysis of radioactive agent composition comprising the synthesis of said radioactive agent according to the method of the invention.

专利号:US-6548276-B2
优先权日:2000-09-06
标题:Enhanced in vitro synthesis of active proteins containing disulfide bonds
发明人：SWARTZ JAMES ROBERT; KIM DONG-MYUNG
权利人:UNIV LELAND STANFORD JUNIOR
摘要:Compositions and methods are provided for the enhanced in vitro synthesis of polypeptides containing disulfide bonds. In order to improve the performance of in vitro protein synthesis reactions, pre-treatment and redox buffering of the reaction mix is performed in order to optimize the redox potential. Exogenous enzymes that enhance protein folding and disulfide bond formation may also be added to the reaction.

专利号:US-9920349-B2
优先权日:2011-11-23
标题:Methods and materials for enzymatic synthesis of mogroside compounds
发明人：LIU YAOQUAN; LEE JUNG YEOP; KHARE MONIKA
权利人:EVOLVA SA
摘要:Methods and materials for enzymatic synthesis of mogroside compounds described.