专利号:US-2023373003-A1 优先权日:2022-05-17 标 题 :Seedless Synthesis of Anisotropic Gold Nanoflowers with Cellular Control and Drug Delivery Applications 发明人:OH EUNKEU; SUSUMU KIMIHIRO; SANGTANI AJMEETA; ROGERS Katherine; NAG OKHIL K; LEE KWAHUN; VURGAFTMAN IGOR; WEIBLEN R JOSEPH; KIM MIJIN; DELEHANTY JAMES B 权利人:US GOV SEC NAVY 摘要:A new seedless synthesis of anisotropic nanoscale gold nanoflower (AuNF) particles uses bidentate thiolate ligands to protect the nanoparticle surface and a combination of reagents (for example, ligand, ascorbic acid, and hydroxide) to synthesis AuNF with controlled size and anisotropic properties. Compared to prior art gold nanospheres, AuNF produced approximately a 15-fold improvement in a drug delivery assay.
专利号:WO-2024199310-A1 优先权日:2023-03-31 标 题 :Method for enzymatic synthesis of capped mrna in one tube 发明人:LI YALI; ZHU HUAXING; ZHANG QINGYI 权利人:NOVOPROTEIN SCIENT INC 摘要:Provided is a method for enzymatic synthesis of capped mRNA in one tube. In the method, the step of purifying mRNA after IVT is omitted, and a capped product having a higher capping efficiency and fewer by-products is obtained. The method provided for enzymatic synthesis of capped mRNA is simple, convenient and short in terms of the process, and is rapid and efficient, so that the production process of a capped mRNA product is simplified, and a production cost is reduced, which is conducive to industrial production.
专利号:US-9884830-B2 优先权日:2004-05-21 标题 :Synthesis of tetracyclines and analogues thereof 发明人:MYERS ANDREW G; CHAREST MARK G; LERNER CHRISTIAN D; BRUBAKER JASON D; SIEGEL DIONICIO R 权利人:HARVARD COLLEGE 摘要:The tetracycline class of antibiotics has played a major role in the treatment of infectious diseases for the past 50 years. However, the increased use of the tetracyclines in human and veterinary medicine has led to resistance among many organisms previously susceptible to tetracycline antibiotics. The modular synthesis of tetracyclines and tetracycline analogs described provides an efficient and enantioselective route to a variety of tetracycline analogs and polycyclines previously inaccessible via earlier tetracycline syntheses and semi-synthetic methods. These analogs may be used as anti-microbial agents or anti-proliferative agents in the treatment of diseases of humans or other animals.
专利号:US-6998484-B2 优先权日:2000-10-04 标 题:Synthesis of purine locked nucleic acid analogues 发明人:KOCH TROELS; JENSEN FLEMMING REISSIG 权利人:SANTARIS PHARMA AS 摘要:The present invention relates to a new method for the synthesis of purine LNA (Locked Nucleic Acid) analogues which provides a higher overall yield. The method comprising a regioselective 9-N purine glycosylation reaction followed by a one-pot nucleophilic aromatic substitution reaction of the 6-substituent in the purine ring and simultaneous nucleophile-induced intramolecular ring closure of the C-branched carbohydrate to form novel purine LNA analogues. The novel strategy is illustrated by the synthesis of the novel compound (1S,3R,4R,7S)-7-benzyloxy-1-methanesulfonylmethyl-3-(guanin-9-yl)-2,5-dioxabicyclo[2.2.1]heptane which is easily converted into (1S,3R,4R,7S)-7-hydroxy-1-hydroxymethyl-3-((2-N-isobutyrylguanin-9-yl)-2,5-dioxabicyclo[2.2.1]heptane after isobutyryl protection of the 2-amino purine group and subsequent substitution of 1-methanesulfonyl with benzoate, debenzoylation and debenzylation.
专利号:US-6329543-B1 优先权日:1999-05-06 标题:Process for synthesis of isobornyl (Meth) acrylate 发明人:KNEBEL JOACHIM; SAAL DORIS 权利人:ROEHM GMBH 摘要:The present invention relates to processes for synthesis of isobornyl (meth)acrylate by reacting camphene with (meth)acrylic acid in the presence of sulfuric acid and at least one compound having inhibiting action. Aqueous sulfuric acid with an acid concentration in the range of 65 to 85 wt % is used. Isobornyl (meth)acrylate can advantageously be distilled from a mixture containing sulfuric acid in the presence of 2,6-di-tert-butyl-alpha-(dialkylamino)-p-cresol.
专利号:US-10364262-B2 优先权日:2012-07-17 标 题 :Method for the synthesis of N-phosphonomethyliminodiacetic acid 发明人:DEVAUX ALBERT; BURCK SEBASTIAN; NOTTE PATRICK 权利人:MONSANTO TECHNOLOGY LLC 摘要:A method for synthesis of N-phosphonoalkyliminodiacetic acid or derivatives thereof by forming a reaction mixture having an acid catalyst, a compound of the following general formula R1—CH2—NX—CH2—R2 and a compound having one or more P—O—P anhydride moieties to form a compound having a formula R1—CH2—N(—CH2PO3R32)(—CH2—R2) wherein in R1—CH2—NX—CH2—R2: X is —CH2—OH or —CH2—COOH; R1 and R2 are independently selected from the group consisting of nitrile, C1-C4 alkyl carboxylate, and carboxylic acid for when X is —CH2—OH, or R1 and R2 are both carbonyl groups linked by a hydrogen substituted nitrogen atom or a C1-C4-alkyl substituted nitrogen atom; and R3 is H, an alkyl group, or an aryl group; the anhydride moieties in the P—O—P anhydride compound have one P atom at the oxidation state (+III) and one P atom at the oxidation state (+III) or (+V); and 2) hydrolyzing the mixture to form N-phosphonomethyliminodiacetic acid or one of its derivatives.
1: Njagi JI, Goia DV. Nitrilotriacetic acid: a novel reducing agent for synthesizing colloidal gold. J Colloid Interface Sci. 2014 May 1;421:27-32. doi: 10.1016/j.jcis.2014.01.025. Epub 2014 Jan 25. doi: 10.1021/ja5063357. Epub 2014 Sep 30. doi: 10.1016/j.chemosphere.2015.02.018. Epub 2015 Mar 3. doi: 10.1002/chem.201406274. Epub 2015 Feb 12. doi: 10.1021/ac303604b. Epub 2013 Mar 4. Epub 2016 Sep 19. doi: 10.1016/j.talanta.2013.07.017. Epub 2013 Jul 15. doi: 10.1021/bc900448f. 11: Watson DS, Platt VM, Cao L, Venditto VJ, Szoka FC Jr. Antibody response to polyhistidine-tagged peptide and protein antigens attached to liposomes via lipid-linked nitrilotriacetic acid in mice. Clin Vaccine Immunol. 2011 Feb;18(2):289-97. doi: 10.1128/CVI.00425-10. Epub 2010 Dec 15. 12: Mero A, Ishino T, Chaiken I, Veronese FM, Pasut G. Multivalent and flexible PEG-nitrilotriacetic acid derivatives for non-covalent protein pegylation. Pharm Res. 2011 Oct;28(10):2412-21. doi: 10.1007/s11095-011-0468-8. Epub 2011 May 25. doi: 10.1016/j.biomaterials.2010.02.063. Epub 2010 Mar 25.
合成参考文献
参考文献:10.3389/fmicb.2011.00107 摘要:Jerse AE, Wu H, Packiam M, Vonck RA, Begum AA, Garvin LE. Estradiol-Treated Female Mice as Surrogate Hosts for Neisseria gonorrhoeae Genital Tract Infections. Front Microbiol. 2011;2():107. 参考文献:10.1371/journal.pone.0021929 摘要:Wu L, Xu L, Liao J, Chen J, Liang Y. Both the C-Terminal Polylysine Region and the Farnesylation of K-RasB Are Important for Its Specific Interaction with Calmodulin. PLoS ONE. 2011 Jul 05;6(7):e21929. doi: 10.1371/journal.pone.0021929. 参考文献:10.1136/gut.2011.241208 摘要:Schwarz P, Kübler JA, Strnad P, Müller K, Barth TF, Gerloff A, Feick P, Peyssonnaux C, Vaulont S, Adler G, Kulaksiz H. Hepcidin is localised in gastric parietal cells, regulates acid secretion and is induced by Helicobacter pylori infection. Gut. 2012 Feb;61(2):193–201. doi: 10.1136/gut.2011.241208. 参考文献:10.4137/aci.s5953 摘要:Narola B, Singh AS, Mitra M, Santhakumar PR, Chandrashekhar TG. A validated reverse phase HPLC method for the determination of disodium EDTA in meropenem drug substance with UV-detection using precolumn derivatization technique. Anal Chem Insights. 2011;6():7–14. 参考文献:10.1186/1746-4811-7-22 摘要:Tsugama D, Liu S, Takano T. A rapid chemical method for lysing Arabidopsis cells for protein analysis. Plant Methods. 2011 Jul 15;7():22.