CAS: 69-79-4; (2R,3R,4R,5R)-2,3,5,6-Tetrahydroxy-4-(((2R,3R,4S,5S,6R)-3,4,5-Trihydroxy-6-(Hydroxymethyl)Tetrahydro-2H-Pyran-2-yl)Oxy)Hexanal

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α-D-Glucopyranosyl-(1->6)-α-D-Glucopyranosyl-(1->6)-α-D-Glucopyranosyl-(1->4)-D-Glucopyranose置于aspergillus Niger Amyloglucosidase体系中,化学反应 6.0H,反应生成 麦芽糖
参考文献:Bioengineering Of Leuconostoc Mesenteroides Glucansucrases That Gives Selected Bond Formation For Glucan Synthesis And/or Acceptor-Product Synthesis
标题:Bioengineering Of Leuconostoc Mesenteroides Glucansucrases That Gives Selected Bond Formation For Glucan Synthesis And/or Acceptor-Product Synthesis
摘要:The Variations In Glucosidic Linkage Specificity Observed In Products Of Different Glucansucrases Appear To Be Based On Relatively Small Differences In Amino Acid Sequences In Their Sugar-Binding Acceptor Subsites. Various Amino Acid Mutations Near Active Sites Of Dsrbcb4 Dextransucrase From Leuconostoc Mesenteroides B-1299Cb4 Were Constructed. A Triple Amino Acid Mutation (S642N/e643N/v644S) Immediately Next To The Catalytic D641 (Putative Transition State Stabilizing Residue) Converted Dsrbcb4 Enzyme From The Synthesis Of Mainly Alpha-(1-> 6) Dextran To The Synthesis Of Alpha-(1-> 6) Glucan Containing Branches Of Alpha-(1-> 3) And Alpha-(1-> 4) Glucosidic Linkages. The Subsequent Introduction Of Mutation V532P/v535I,Located Next To The Catalytic D530 (Nucleophile),Resulted In The Synthesis Of An Alpha-Glucan Containing Increased Branched Alpha-(1-> 4) Glucosidic Linkages (Approximately 11%). The Results Indicate That Mutagenesis Can Guide Glucansucrase Toward The Synthesis Of Various Oligosaccharides Or Novel Polysaccharides With Completely Altered Linkages Without Compromising High Transglycosylation Activity And Efficiency.
DOI:10.1021/jf104629G

专利信息

专利号:WO-2023041957-A1
优先权日:2021-09-16
标题 :Efficient process for the synthesis of iron (iii) carbohydrate complexes
发明人：MAMIDYALA SREEMAN KUMAR; NANDIGALA HEMANTH; TUMMURU MURALI KRISHNA REDDY; G V HANUMAN RAJU; GOUD B SATYANARAYANA
权利人:VIRCHOW BIOTECH PRIVATE LTD
摘要:The present invention relates to novel processes for the preparation of iron (III) carbohydrate complex. Thus, for example, starch was oxidised using various oxidizing agents such as hydrogen peroxide, sodium hypochlorite, oxone to obtain oxidized sugar derivative. The oxidized product was reacted with ferric hydroxide in water at 100°C to produce iron (III) carbohydrate complex. The invention results in the formation of ferric carbohydrate complex with consistent average molecular weight and iron content.

专利号:US-11473116-B2
优先权日:2018-02-23
标题 :Synthesis of glucan comprising alpha-1,3 glycosidic linkages with phosphorylase enzymes
发明人：YU ZHEYONG; KRALJ SLAVKO; ZHANG ZHENGHONG; HOWE LAURIE A
权利人:NUTRITION & BIOSCIENCES USA 4 INC
摘要:Reaction compositions are disclosed herein comprising at least water, beta-glucose -1-phosphate (beta-G1P), an acceptor molecule, and an alpha-1,3-glucan phosphorylase enzyme. These reactions can synthesize oligosaccharides and polysaccharides with alpha-1,3 glycosidic linkages. Further disclosed are alpha-1,3-glucan phosphorylase enzymes and methods of use thereof.

专利号:US-2004091900-A1
优先权日:2002-06-21
标 题:Materials and methods for providing plants with increased resistance to environmental stress
发明人：GUY CHARLES L; KAPLAN FATMA; SUNG DONG YUL
摘要:The subject invention pertains to materials and methods for protecting plants and plant organelles, such as chloroplasts, during thermal (heat and cold) stress, and other forms of environmental stress such as water and salt stress. In one embodiment, a plant is transformed with a polynucleotide that encodes a protein that produces, catalyzes the synthesis of or results in the production of maltose or a maltose alcohol. In an exemplified embodiment, the polynucleotide encodes a β-amylase enzyme that is localized at the chloroplast. The subject invention also concerns plants and plant tissue transformed with a polynucleotide that encodes a protein that produces or results in the production of maltose or a maltose alcohol.

专利号:US-9339782-B1
优先权日:2004-11-18
标题 :Electrode array device having an adsorbed porous reaction layer
发明人：GINDILIS ANDREI
权利人:CUSTOMARRAY INC
摘要:There is disclosed an electrode array device having an adsorbed porous reaction layer for improved synthesis quality. The array comprises a plurality of electrodes on a substrate, wherein the electrodes are electronically connected to a computer control system. The array has an adsorbed porous reaction layer on the plurality of electrodes, wherein the adsorbed porous reaction layer comprises a chemical species having at least one hydroxyl group. In the preferred embodiment, the reaction layer is sucrose. A method for preparing an electrode array for improved synthesis quality is disclosed. The method comprises a cleaning method and a method of attachment of a reaction layer. The cleaning method comprises a plasma cleaning method and a chemical cleaning method. The reaction layer is attached after cleaning by exposing the microarray to a solution containing the chemical species having at least one hydroxyl group.