CAS: 10510-54-0; 5,9-Diaminobenzo[a]Phenoxazin-7-Ium Acetate

专利信息

专利号:US-5128419-A
优先权日:1990-08-20
标 题:Synthesis of tagged polymers by post-polymerization (trans) amidation reaction
发明人：FONG DODD W; HOOTS JOHN E
权利人:NALCO CHEMICAL CO
摘要:Polymers tagged with pendant fluorescent groups are prepared by the (trans)amidation derivatization of pre-existing polymers having carbonyl-type pendant groups. Polymers having pendant amide groups wherein the amide nitrogen is substituted with fluorescent moieties, prepared by (trans)amidation drrivatization, are provided. Polymers having pendant (sulfonated) napthalene moieties substituted to amide nitrogen are provided.

专利号:US-5260386-A
优先权日:1990-08-20
标 题 :Synthesis of tagged polymers by post-polymerization (trans) amidation reaction
发明人：FONG DODD W; HOOTS JOHN E
权利人:NALCO CHEMICAL CO
摘要:A polymer having pendant fluorescent groups is prepared by (trans)amidation of a preformed polymer by reaction with an amine-containing organic fluorescent composition of the Formula III Formula III wherein one of R5 and R6 may be hydrogen, and wherein within at least one of R5 and R6, or within R5 and R6 taken together, is an organic fluorescent group wherein the organic fluorescent group includes a polynuclear aromatic ring system. A degree of (trans)amidation derivatization of the polymer is accomplished by heating the admixture of polymer and fluorescent agent for a sufficient period of time. A second amine-containing post-polymerization derivatization agent other than the organic fluorescent composition of Formula III is added to the admixture or to at least a portion of the reaction product of the (trans)amidation derivatization, and is heated therewith to accomplish a degree of sequential or simultaneous post-polymerization derivatization with the second amine-containing post-polymerization derivatization agent.

专利号:US-7915016-B2
优先权日:2004-05-28
标题 :cDNA production from cells after laser microdissection
发明人：LISS BIRGIT
权利人:UNIV MARBURG PHILIPPS
摘要:The present invention provides a new procedure for the synthesis of cDNA from single cells after microdissection. It has the advantage that it is cost-efficient and can be carried out quickly with only few steps, even by less skilled laboratory employees. For the first time, the time-consuming and risky step of RNA isolation is omitted during cDNA synthesis from single cells by performing lysis and cDNA synthesis in the same reaction tube and in one buffer solution, which provides reliable and contamination-free results. The buffer is composed of NP40, carrier-RNA and Super RNAsin, as well as dNTPs and cDNA synthesis primers.

专利号:US-5216086-A
优先权日:1990-08-20
标 题:Synthesis of tagged polymers by post-polymerization (trans)amidation reaction