CAS: 653-63-4; ((2R,3S,5R)-5-(6-Amino-9H-Purin-9-yl)-3-Hydroxytetrahydrofuran-2-yl)Methyl Dihydrogen Phosphate

相似化合物

2922-74-9 61-19-8 72003-83-9

上下游产品

CAS号958-09-8 2'-脱氧腺苷 | CAS号1927-31-7 2′-脱氧腺苷-5′-三磷酸 钠盐 | CAS号2793-06-8 [[(2R,3S,5R)-5-... | CAS号60508-81-8 Etheno-2'-deoxy...

3'-O-Acetyl-N-Benzoyl-2'-Deoxyadenosine置于ammonium Hydroxide,Tps-Taz体系中,用 吡啶,水 作为反应溶剂,化学反应 8.33H,反应生成 2'-脱氧腺苷-5'-单磷酸
参考文献:核苷磷酸化的新方法
标题:核苷磷酸化的新方法
摘要:开发了一种新的磷酸化试剂2-O-(4,4'-二甲氧基三苯甲基)乙基磺酰lethan-2'-基磷酸酯(1),用于磷酸化核苷的伯醇和仲醇.在许多研究的实例中,磷酸化步骤的收率非常好(约80%至95%).该方法不仅在核苷和核苷酸化学中,而且在诸如碳水化合物和氨基酸的生物分子的磷酸化中都有广泛的应用潜力.
DOI:10.1016/s0040-4039(00)01251-X

专利信息

专利号:US-8058414-B2
优先权日:2008-04-29
标题 :Unnatural polymerase substrates that can sustain enzymatic synthesis of double stranded nucleic acids from a nucleic acid template and methods of use
发明人：MENCHEN STEVEN; ROSENBLUM BARNETT; KENNEY PAUL; KHAN SHOEB; MA ZHAOCHUN; CHEN JER-KANG; LAM JOE Y; HUANG BOLI
权利人:MENCHEN STEVEN; ROSENBLUM BARNETT; KENNEY PAUL; KHAN SHOEB; MA ZHAOCHUN; CHEN JER-KANG; LAM JOE Y; HUANG BOLI; LIFE TECHNOLOGIES CORP
摘要:Nucleotide analogs that can sustain the enzymatic synthesis of double-stranded nucleic acid from a nucleic template are described. The nucleotide analogs include: (i) a base selected from the group consisting of adenine, guanine, cytosine, thymine, uracil and their analogs; (ii) a label attached to the base or analog of the base via a cleavable linker; (iii) a deoxyribose; and (iv) one or more phosphate groups. The linker and/or the label inhibits template directed polymerase incorporation of a further nucleotide substrate onto an extended primer strand. In addition, cleavage of the linker leaves a residue attached to the base which is not present in the natural nucleotide and which does not inhibit extension of the primer strand. The nucleotide analogs can therefore be used as reversible terminators in sequencing by synthesis methods without blocking the 3′ hydroxyl group. Methods of sequencing DNA using the substrates are also described.

专利号:US-8314209-B2
优先权日:2007-12-12
标 题 :Lipid-assisted synthesis of polymer compounds and methods for their use
发明人：RAJAMANI SUDHA; OLASAGASTI FELIX; DEAMER DAVID W; BENNER SEICO
权利人:RAJAMANI SUDHA; OLASAGASTI FELIX; DEAMER DAVID W; BENNER SEICO; UNIV CALIFORNIA
摘要:The invention herein disclosed provides for methods for the synthesis of polymers from monomers. In particular the method provides for the synthesis of polynucleotides from mononucleotides in the absence of catalytic enzymes. The method comprises providing an aqueous solution having a plurality of phospholipid molecules and monomer molecules; subjecting the aqueous solution to fluctuating temperature conditions; subjecting the aqueous solution to fluctuating cycles of drying and hydrating conditions; subjecting the aqueous solution to fluctuating [H + ] conditions; the fluctuating conditions thereby allowing formation of a chemical bond between at least two monomers to create a polymer. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.

专利号:US-2009118140-A1
优先权日:2007-03-15
标 题 :Method and system for assembly of macromolecules and nanostructures
发明人：SUZARA VINCENT
权利人:SUZARA VINCENT
摘要:A template-based system enables the assembly of macromolecules and nanostructures. The template system comprises a plurality of single strand DNA molecules which are substantially parallel, substantially inline each from one end, and substantially equally spaced apart, wherein each DNA molecule has a distinguishable length and a known sequence. The system can be used for the precise, accurate, and efficient synthesis of peptides, proteins and enzymes.

专利号:US-10378035-B2
优先权日:2015-04-22
标 题 :Synthesis of transcripts using Syn5 RNA polymerase
发明人：ZHU BIN; RICHARDSON CHARLES C; TABOR STANLEY
权利人:HARVARD COLLEGE
摘要:Methods of in vitro transcription using cyanophage Syn5 RNA polymerase (RNAP) or mutants thereof and transcription conditions are provided.

专利号:US-4728730-A
优先权日:1984-09-12
标题:Synthesis of nucleoside thiophosphoanhydrides
发明人：FREY PERRY A; HO HSU-TSO
权利人:WISCONSIN ALUMNI RES FOUND
摘要:A method for preparing sulfur and/or selenium containing phosphoanhydrides that have at least a triphosphate moiety is disclosed, together with a cyclo-intermediate formed during the reaction. The method includes reacting a first compound having an available and reactive group selected from phosphorodihalidate, thiophosphorodihalidate, and selenic phosphorodihalidate (the compound having a remaining portion that does not interfere with the reaction), with a second compound having an available and reactive group selected from phosphate, thiophosphate, and selenic phosphate (the second compound also having a remaining segment that does not interfere with the reaction). The selection of the reactive groups is such that at least one of the reactive groups is the thio or selenic variant, and the selection of the remaining portion and segment are such that at least one is attached to a linking group selected from phosphate, thiophosphate, and selenic phosphate that links it to the available and reactive group. The reaction appears to be best suited for creating nucleoside thiotriphosphates.

专利号:US-2025084410-A1
优先权日:2015-01-12
标 题:Incorporation of unnatural nucleotides and methods thereof
发明人：PTACIN JEROD; MALYSHEV DENIS A; CAFFARO CAROLINA E
权利人:SYNTHORX INC
摘要:Disclosed herein are methods, compositions and kits for the synthesis of proteins which comprises unnatural amino acids that utilize a mutant tRNA.