CAS: 86030-43-5; 1-Chloro-N,N-Diisopropyl-1-Methoxyphosphanamine

上下游产品

methyl N,N-diisopropylphosphonamidate methyl dichlorophosphite diisopropylamine Dimethyl chlorophosphate2-benzoyl-2',3'-bis-O-(tert-butyldimethylsilyl)guanosine 5'-O-(methyl N,N-diisopropylphosphoramidite)N2-benzoyl-2',3'-bis-O-(tert-butyldimethylsilyl)guanosine 5'-O-(methyl N,N-diisopropylphosphoramidite) 4-nitrophenethylmethoxydiisopropylaminophosphine Menthyl-methyl-diisopropylphosphoramidit 2-isobutyryl-2'-deoxyguanosine-3'-yl]-(N,N'-diisopropyl)methylphosphoramidite[5'-O-(4,4'-dimethoxytrityl)-N2-isobutyryl-2'-deoxyguanosine-3'-yl]-(N,N'-diisopropyl)methylphosphoramidite

甲醇,二异丙胺置于吡啶,三氯化磷体系中,用 乙醚 作为反应溶剂,化学反应 25.5H,以56%的收率获得产物氯(二异丙基氨基)甲氧基膦
参考文献:Lipidomics Characterization Of Biosynthetic And Remodeling Pathways Of Cardiolipins In Genetically And Nutritionally Manipulated Yeast Cells
标题:Lipidomics Characterization Of Biosynthetic And Remodeling Pathways Of Cardiolipins In Genetically And Nutritionally Manipulated Yeast Cells
摘要:Cardioipins (Cls) Are Unique Tetra-Acylated Phospholipids Of Mitochondria And Define The Bioenergetics,And Regulatory Functions Of These Organelles. An Unresolved Paradox Is The High Uniformity Of Cl Molecular Species (Tetra-Linoleoyl-Cl) In The Heart,Liver,And Skeletal Muscles In Contrast To Their High Diversification In The Brain. Here,We Combined Liquid Chromatography Mass-Spectrometry-Based Phospholipidomics With Genetic,And Nutritional Manipulations To Explore Cls Biosynthetic Vs Postsynthetic Remodeling Processes In S. Cerevisiae Yeast Cells. By Applying The Differential Phospholipidomics Analysis,We Evaluated The Contribution Of Cld1 (Cl-Specific Phospholipase A) And Taz1 (Acyl-Transferase) As The Major Regulatory Mechanisms Of The Remodeling Process. We Further Established That Nutritional "Pressure" By High Levels Of Free Fatty Acids Triggered A Massive Synthesis Of Homoacylated Molecular Species In All Classes Of Phospholipids,Resulting In The Preponderance Of The Respective,Homoacylated Cls. We Found That Changes In Molecular Speciation Of Cls Induced By Exogenous C18-Fatty Acid'S,(C18:1 And C18:2) Wild-Type (Wt) Cells Did Not occur In Any Of The Remodeling Mutant Cells,Including Dd1 Delta,Taz1 Delta,And Dd1 Delta Taz1 Delta. Interestingly,Molecular Speciation Of Cls In Wt And Double Mutant Cells Dd1 Delta Taz1 Delta Was Markedly Different. Given That The Bioenergetics Functions Are Preserved In The,Double Mutant,This Suggests That The Accumulated Mlcl-Rather Than The Changed Cl Speciation-Are The Likely Major Contributors To The Mitochondrial Dysfunction In Taz1 Delta Mutant Cells (Also Characteristic Of Barth Syndrome): Biochemical Studies Of Cld1 Specificity And Computer Modeling Confirmed The Hydrolytic Selectivity Of The Enzyme Toward C16-Cl Substrates And The Preservation Of C18:1-Containing Cl Species.
DOI:10.1021/acschembio.6B00995

专利信息

专利号:US-5324831-A
优先权日:1988-04-06
标题:Phosphoramidite reagent for chemical synthesis of modified DNA
发明人：MARQUEZ VICTOR E; GODDARD AMANDA J
权利人:US HEALTH
摘要:5,6-dihydro-5-azacytidine phosphoramidite is useful in the synthesis of oligonucleotides and DNA containing dihydro-5-aza- and 5-azacytosine bases. The modified oligonucleotides which contain 5-azacytosine residues at specific sites can be used to determine the mechanism of selective gene activation and the relationship existing between the presence of the triazine base and inhibition of DNA methylation.

专利号:US-4849513-A
优先权日:1983-12-20
标 题 :Deoxyribonucleoside phosphoramidites in which an aliphatic amino group is attached to the sugar ring and their use for the preparation of oligonucleotides containing aliphatic amino groups
发明人：SMITH LLOYD M; FUNG STEVEN; KAISER ROBERT J
权利人:CALIFORNIA INST OF TECHN
摘要:The invention consists of compounds and methods for the synthesis of oligonucleotides which contain one or more free aliphatic amino groups attached to the sugar moieties of the nucleoside subunits. The synthetic method is versatile and general, permitting amino groups to be selectively placed at any position on oligonucleotides of any composition or length which is attainable by current DNA synthetic methods. Fluorescent dyes or other detectable moieties may be covalently attached to the amino groups to yield the corresponding modified oligonucleotide.

专利号:US-5118802-A
优先权日:1983-12-20
标 题:DNA-reporter conjugates linked via the 2' or 5'-primary amino group of the 5'-terminal nucleoside
发明人：SMITH LLOYD M; FUNG STEVEN; KAISER JR ROBERT J
权利人:CALIFORNIA INST OF TECHN
摘要:The invention consists of compounds and methods for the synthesis of oligonucleotides which contain one or more free aliphatic amino groups attached to the sugar moieties of the nucleoside subunits. The synthetic method is versatile and general, permitting amino groups to be selectively placed at any position on oligonucleotides of any composition or length which is attainable by current DNA synthetic methods. Fluorescent dyes or other detectable moieties may be covalently attached to the amino groups to yield the corresponding modified oligonucleotide.

专利号:US-6340749-B1
优先权日:1995-10-06
标题:Preparation of nucleoside phosphoramidites and oligonucleotide synthesis
发明人：ZHANG ZHAODA; TANG JIN-YAN
权利人:AVECIA BIOTECHNOLOGY INC
摘要:The invention provides novel bifunctional phosphitylating reagents and their application in in situ preparation of 5'-protected nucleoside phosphoramidites and synthesis of oligonucleotides. Bifunctional phosphitylating reagents according to the invention react quickly with nucleosides under neutral or weakly basic conditions, without an additional activation step. In addition, the bifunctional phosphitylating reagents according to the invention generate chemoselectively the corresponding nucleoside phosphoramidites in situ, without need to purify the nucleoside phosphoramidites before using them in oligonucleotide synthesis. Finally, the bifunctional phosphitylating reagents according to the invention are relatively stable and easy to handle.

专利号:US-5869644-A
优先权日:1992-04-15
标题:Synthesis of diverse and useful collections of oligonucleotidies
发明人：SHORTLE DAVID R; SONDEK JOHN
权利人:UNIV JOHNS HOPKINS
摘要:A new technique for generating mixtures of oligonucleotides in a single automated synthesis is taught. The method can be used to prepare mixed oligonucleotides ideally suited for creation of useful mixtures of oligo- or polypeptides or proteins. Additionally, the technique enables insertion and/or substitution and/or deletion of a nucleotide sequence at one or more sites. For protein mutagenesis, a trinucleotide can be inserted or substituted at codon boundaries. The invented technique makes possible the encoding of all possible single amino acid insertions, or any desired mixture of substitutions and insertions.

专利号:WO-9003381-A1
优先权日:1988-09-20
标 题 :Method of synthesis of alpha oligoribonucleotides and compounds useful for the method
发明人：RAYNER BERNARD; IMBACH JEAN-LOUIS
权利人:CENTRE NAT RECH SCIENT
摘要:The invention concerns a method of synthetizing alpha oligoribonucleotide compounds in which synthesis is carried out with phosphoramidite on a solid support, as well as compounds useful in said method and the compounds obtained. According to the invention, protected alpha ribonucleoside monomers, substituted by phosphoramidite groups in the 3' position, are assembled, the hydroxyl function in the 2' position of the ribose of said monomer being protected by the Ctmp or TBDMS group.