专利号:US-7344863-B2 优先权日:1998-03-13 标题:Methods for producing polypeptides through enhanced synthesis of encoding nucleic acid molecules 发明人:LI WU-BO; JESSEE JOEL A; SCHUSTER DAVID; XIA JIULIN; GEBEYEHU GULILAT 权利人:INVITROGEN CORP 摘要:The present invention is directed to compositions and methods for enhancing synthesis of nucleic acid molecules, particularly GC-rich nucleic acid molecules. Specifically, the invention provides compositions comprising one or more nitrogen-containing organic compounds having a formula selected from the group consisting of formula I and formula II (or salts or derivatives thereof), preferably 4-methylmorpholine N-oxide or betaine (carboxymethyltrimethylammonium), and further comprising one or more compounds selected from the group consisting of proline and an N-alkylimidazole compound, and more preferably proline, 1-methylimidazole or 4-methylimidazole. The invention further relates to methods for enhanced, high-fidelity synthesis of nucleic acid molecules, including via amplification (particularly PCR), reverse transcription, and sequencing methods. The invention also relates to nucleic acid molecules synthesized by these methods, to fragments or derivatives thereof, and to vectors and host cells comprising such nucleic acid molecules, fragments, or derivatives. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the compositions of the invention.
专利号:US-9267213-B1 优先权日:2005-03-25 标题:Electrochemical deblocking solution for electrochemical oligomer synthesis on an electrode array 发明人:MAURER KARL; COOPER JOHN J 权利人:CUSTOMARRAY INC 摘要:There is disclosed an electrochemical deblocking solution for use on an electrode microarray. There is further disclosed a method for electrochemical synthesis on an electrode array using the electrochemical deblocking solution. The solution and method are for removing acid-labile protecting groups for synthesis of oligonucleotides, peptides, small molecules, or polymers on a microarray of electrodes while substantially improving isolation of deblocking to active electrodes. The method comprises applying a voltage or a current to at least one electrode of an array of electrodes. The array of electrodes is covered by the electrochemical deblocking solution.
专利号:US-10407676-B2 优先权日:2014-12-09 标题 :High efficiency, small volume nucleic acid synthesis 发明人:POEHMERER THOMAS; KUHN PHILLIP; NOTKA FRANK; ZEIDLER ANDREAS; HEIL KORBINIAN; TREFZER AXEL; FONNUM GEIR; KATZEN FEDERICO; ANDERSSON KRISTIAN 权利人:LIFE TECHNOLOGIES CORP; THERMO FISHER SCIENT GENEART GMBH; LIFE TECH AS 摘要:The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).
专利号:WO-9946400-A1 优先权日:1998-03-13 标题:Compositions and methods for enhanced synthesis of nucleic acid molecules
专利号:JP-2002505886-A 优先权日:1998-03-13 标题 :Compositions and methods for enhancing the synthesis of nucleic acid molecules
专利号:EP-1062360-A1 优先权日:1998-03-13 标 题 :Compositions and methods for enhanced synthesis of nucleic acid molecules