CAS: 6375-55-9; Disodium,5-[(3-Methyl-5-Oxo-1-Phenyl-4H-Pyrazol-4-yl)Diazenyl]-2-[4-[(3-Methyl-5-Oxo-1-Phenyl-4H-Pyrazol-4-yl)Diazenyl]-2-Sulfonatophenyl]Benzenesulfonate

专利信息

专利号:WO-2024097977-A1
优先权日:2022-11-04
标题:Silk nanoparticle synthesis: tuning size, dispersity, and surface chemistry for drug delivery
发明人：SHAIDANI SAWNAZ; FALCUCCI THOMAS; FOSTER OLIVIA; SAHOO JUGAL KISHORE; HASTURK ONUR; KAPLAN DAVID L; JACOBUS CHARLOTTE S
权利人:TUFTS COLLEGE
摘要:Methods disclosed herein relate to generating silk nanoparticles with a low polydispersity index (PDI). Methods include adding a silk solution dropwise into a volatile solvent that is miscible with water, thereby forming a precipitate-bearing solution and applying shear forces to the precipitate-bearing solution for a length of time sufficient to achieve evaporation of at least 95% of the volatile solvent, thereby producing a population of silk fibroin nanoparticles in water having a polydispersity index (PDI) of 0.35 or less, including but not limited to, a PDI of 0.330 or less, 0.325 or less, 0.315 or less, 0.310 or less, 0.30 or less, 0.275 or less, 0.250 or less, 0.225 or less, or 0.200 or less. Parameters related to silk fibroin molecular weight, silk fibroin concentration, shear force application, and temperature may all be modified to achieve silk nanoparticles of a particular size exhibiting particular PDIs.