专利号:WO-2024165450-A1 优先权日:2023-02-09 标题:Method of chemical synthesis of single-chain antibody fragments and products thereby obtained 发明人:FIGINI MARIANGELA; LUISON ELENA 权利人:GLYTECH INC; FIGINI MARIANGELA 摘要:A new method is disclosed for the protein full-chemical synthesis of single-chain antibody fragments (scFv); the obtained products are structurally close and functionally equivalent to their biological counterpart, being however, advantageously free of impurities and more reproducible in properties. The method includes the general steps of: (a) separately synthetizing sub-sequences (peptide fragments) (b) sequentially assembling the sub-sequences obtained in step (a) in the order as they appear in the target scFv amino acid sequence, and (c) performing oxidative folding of the assembled whole peptide molecule obtained in step (b) and dialyzing the resulting product in a buffer.
专利号:US-6355455-B1 优先权日:1997-07-09 标题 :Method for enzymatic synthesis of sucrose esters 发明人:PAULY GILLES; ENGASSER JEAN-MARC; GHOUL MOHAMMED 权利人:SEROBIOLOGIQUES LAB SA 摘要:A method for the enzymatic synthesis of sucrose ester, comprises introducing, in an adapted reactor and so as to form a reaction medium, predetermined amounts of an organic solvent, a sugar or a sugar derivative, a compound donor of acyl groups and an enzymatic catalyst, the amount of at least one constituent of the reaction mixture being deficient, in controlled addition during the reaction of additional amounts of the deficient constituent(s), and finally purifying the resulting sucrose esters at least by separating the fine enzymatic particles from the solvent.
专利号:US-2023212621-A1 优先权日:2020-06-09 标题:Process for synthesis of poly-gamma-glutamic acid 发明人:DHARNE MAHESH SHANTAPPA; NAIR PRANAV GIRIJAVALLABHAN 权利人:COUNCIL SCIENT IND RES 摘要:The present invention overcomes the problem of processes yielding poor quantities of poly-gamma-glutamic-acid (γ-PGA) by providing a process and a novel medium for synthesis of γ-PGA in the presence of Bacillus paralicheniformis MCC 196. Wherein, the novel medium comprises tomato waste alone for cost effective and greener production of γ-PGA with yield of 40 g/L. In another process, non-sterile fermentation employs the use of a medium high in sugar content. Wherein, sucrose is used in concentrations up to 50% to obtain more than 280 g/L of poly gamma glutamic acid. The disclosed process is scalable and provides immense economic advantages.
专利号:US-9873905-B2 优先权日:2014-03-07 标题:Chemoenzymatic synthesis of trehalose analogs 发明人:SWARTS BENJAMIN M; WOODRUFF PETER 权利人:CENTRAL MICHIGAN UNIV; UNIV OF MAINE SYSTEM 摘要:The present invention provides methods of synthesizing trehalose analogs; methods of detecting mycobacteria, and trehalose analogs.
专利号:WO-2025066562-A1 优先权日:2023-09-27 标 题 :Sugar backbone polymer and synthesis method 发明人:CHEN GUOSONG; Xue Cece; RAO ZIKUN 权利人:UNIV FUDAN 摘要:A polymer material, specifically a sugar backbone polymer and a synthesis method. A new type of sugar backbone polymer is prepared using a condensation and polymerization method. An addition reaction of alcohol-olefin is introduced into the synthesis of the sugar backbone polymer, to form a new functional primary sugar chain alternating polymer having alternating sugar and aliphatic units. It overcomes shortcomings of existing artificial chemical synthesis methods such as difficulty in controlling the stereoselectivity of the product, low molecular weight, cumbersome monomer preparation and few types. The molecular weight of the prepared polymer can reach 71,067 g/mol, and monomer preparation is simple.
专利号:US-8877171-B2 优先权日:2010-02-03 标 题 :Polyanionic multivalent macromolecules for intracellular targeting of proliferation and protein synthesis 发明人:LICHA KAI; SCHIRNER MICHAEL; WELKER PIA; HAAG RAINER; WEINHART MARIE; PAULUS FLORIAN 权利人:LICHA KAI; SCHIRNER MICHAEL; WELKER PIA; HAAG RAINER; WEINHART MARIE; PAULUS FLORIAN; MIVENION GMBH 摘要:The present invention relates generally to methods and compositions for targeting of intracellular molecules involved in proliferation and protein synthesis of activated cells using polyanionic multivalent macromolecules. In particular aspect, multiple sulfate groups linked to polyol are specifically targeted to the cytoplasm and nucleus of proliferating and activated cells. The invention further comprises novel polyanionic macromolecular compounds and formulations.
1: Santamaria C, Biegas KJ, Kim CY, Cabral J, Lee JR, Swarts BM, Siegrist MS. Trehalose dimycolate inhibits phagosome maturation and promotes intracellular M. tuberculosis growth via noncanonical SNARE interaction. bioRxiv [Preprint]. 2024 Dec 16:2024.12.16.627577. doi: 10.1101/2024.12.16.627577. 2: Hedjazi S, Miyamoto T, Ahn JE, Sitcheran RM, Amrein H. Glucose-6-phosphatase is required for organelle reorganization, energy metabolism and motility of Drosophila sperm. bioRxiv [Preprint]. 2024 Dec
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