CAS: 302-84-1; Dl-Serine

相似化合物

312-84-5 56-45-1 10433-52-0

上下游产品

CAS号5445-44-3 O-苄基-DL-丝氨酸 | CAS号141-46-8 羟乙醛 | CAS号56-45-1 L-丝氨酸 | CAS号1738-71-2 benzyl α-amino-... | CAS号50-00-0 甲醛 | CAS号56-40-6 甘氨酸 | CAS号6168-72-5 DL-氨基丙醇 | CAS号312-84-5 D-丝氨酸(mM/ml) | CAS号77287-34-4 甲亚胺酸 | CAS号302-72-7 DL-2-氨基丙酸 | CAS号3940-27-0 DL-丝氨酸乙酯盐酸盐 | CAS号473-81-4 DL-甘油酸 | CAS号50517-10-7 N-乙酰基6-氯色氨酸 | CAS号127-17-3 丙酮酸 | CAS号7664-41-7 氨 | CAS号50-00-0 甲醛 | CAS号56-40-6 甘氨酸 | CAS号849585-22-4 2-羟基丙酸 | CAS号144-62-7 草酸 | CAS号75-07-0 乙醛

L-丝氨酸置于磷酸吡哆醛,Dictyostelium Discoideum Serine Racemase,5'-三磷酸腺苷,Magnesium Chloride体系中,用 Aq. Buffer 作为反应溶剂,化学反应 0.08H,反应生成 Dl-丝氨酸
参考文献:盘基网柄菌丝氨酸消旋酶的催化机理
标题:盘基网柄菌丝氨酸消旋酶的催化机理
摘要:盘基网柄菌的真核丝氨酸消旋酶是ii型吡ido醛5'-磷酸(plp)依赖性折叠酶,可催化丝氨酸的两个异构体的消旋和脱水.在本研究中,通过定点诱变研究了酶的活性位点残基的催化机理和作用.将plp结合赖氨酸(k56)突变为丙氨酸消除了丝氨酸消旋酶和脱水酶的活性.d-和l的孵育-丝氨酸与所产生的突变酶k56A导致plp-丝氨酸外部醛亚胺的积累,而形成的丙酮酸,α-氨基丙烯酸酯,对映体丝氨酸和醌类中间体的量较少.位于k56相对plp平面另一侧的ser81(s81)的丙氨酸突变将酶从丝氨酸消旋酶转变为1-丝氨酸脱水酶.s81A没有消旋酶活性并且具有显着降低的d-丝氨酸脱水酶活性,但是它完全保留了其1-丝氨酸脱水酶活性.水分子(或多个)在s81A突变体酶的活性位点很可能开车d-丝氨酸通过抽象的α-氢脱水d-丝氨酸.我们的数据表明,抽象和加成α-氢与升-和ð-丝氨酸由k56和s81处进行的si-和重新分别-Sides,Plp的.
DOI:10.1007/s00726-012-1442-4

专利信息

专利号:US-12421534-B2
优先权日:2021-11-10
标 题 :Engineered enzymes and method for the synthesis of diverse tyrosine analogs
发明人：ALMHJELL PATRICK J; ARNOLD FRANCES H
权利人:CALIFORNIA INST OF TECHN
摘要:Provided herein is an engineered tryptophan synthase β-subunit (TrpB) that catalyzes the synthesis of tyrosine, tyrosine analogs, or salts thereof. Also provided herein are methods for preparing tyrosine, tyrosine analogs, or a salt thereof using the engineered TrpB described herein.

专利号:WO-2010103857-A1
优先权日:2009-03-12
标 题 :Method for solid-phase synthesis of glycopeptide using silicon-containing protecting group and synthesis device
发明人：NISHIMURA SHIN-ICHIRO; SHIMAWAKI KEN
权利人:UNIV HOKKAIDO NAT UNIV CORP; NISHIMURA SHIN-ICHIRO; SHIMAWAKI KEN
摘要:Disclosed are a novel method for the synthesis of a glycopeptide by which glycopeptides of various types can be deprotected and excised from a resin, each under weakly acidic to weakly basic conditions, without causing the problems of the epimerization of an amino acid at the a-position and the ß-elimination of a sugar residue; a synthesis device therefor; and a synthesis intermediate to be used in synthesizing a glycopeptide. Specifically disclosed are a method for the solid-phase synthesis of a glycopeptide which comprises a step for obtaining a glycopeptide derivative wherein the N-terminus is protected, the C-terminus is immobilized to a solid phase via a silicon linker, and a reactive group carried by a sugar residue and a reactive group carried by an amino acid side chain constituting a peptide are protected by silicon-containing groups, and a step for obtaining the glycopeptide by deprotecting said glycopeptide derivative and releasing the same from the solid phase by treating the glycopeptide derivative with an agent for removing the silicon-containing groups and the silicon linker; a synthesis intermediate to be used in the step for obtaining the glycopeptide derivative in the aforesaid method; and a device for the solid-phase synthesis of a glycopeptide.

专利号:US-6350595-B1
优先权日:1997-10-28
标题:Synthesis of polynucleotides having random sequences
发明人：NEUNER PHILIPPE
权利人:ISTITUTO DI RICERCHE DI MOLECO
摘要:Subject of this invention is a process for the chemical synthesis of polynucleotides having totally or partially random sequences, based on the utilization, as synthesis monomer units for the random sequence part, or presynthesized mononucleotides and dinucleotides. Said synthesis is carried out on separate supports, so that on each of those supports is alternated at least one reaction cycle wherein a mixture of said dinucleotides is bound, with at least one reaction cycle wherein a mononucleotide is bound, and that in a preferred embodiment at the end of the n cycles required for a codon synthesis, the supports are mixed and then redivided into one or more reaction containers. The resulting polynucleotides are such that, for the random sequence part, each trinucleotide unit is fit to match only a limited number of codons, predefined for each unity in number and sequence, and the genetic code degeneracy effects can thus be eliminated. FIG. 1 shows the chemical structure of the dinucleotides utilized as monomer units for the synthesis of codons forming the final sequence.

专利号:WO-9312076-A1
优先权日:1991-12-13
标题:Reagents for automated synthesis of peptide analogs
发明人：WEBB THOMAS ROY
权利人:CORVAS INT INC
摘要:Reagents suitable for synthesis of peptide analogs using automated peptide synthesis and procedures for synthesis of peptide analogs are provided.

专利号:US-10865390-B2
优先权日:2018-02-12
标 题 :Alcohol dehydrogenase mutant and application thereof in synthesis of diaryl chiral alcohols
发明人：NI YE; ZHOU JIEYU; XU GUOCHAO; WANG YUE
权利人:UNIV JIANGNAN
摘要:The present disclosure discloses an alcohol dehydrogenase mutant and application thereof in synthesis of diaryl chiral alcohols, and belongs to the technical field of bioengineering. The alcohol dehydrogenase mutant of the present disclosure has excellent catalytic activity and stereoselectivity, and may efficiently catalyze the preparation of a series of chiral diaryl alcohols in R- and S-configurations. By coupling alcohol dehydrogenase of the present disclosure to glucose dehydrogenase or formate dehydrogenase, the synthesis of chiral diaryl alcohol intermediates of various antihistamines may be achieved. Compared with the prior art, a method for preparing diaryl chiral alcohols through asymmetric catalytic reduction using the alcohol dehydrogenase of the present disclosure has the advantages of simple and convenient operation, high substrate concentration, complete reaction and high product purity, and has great industrial application prospects.

专利号:US-2024035023-A1
优先权日:2022-06-24
标题 :Computer implemented method for engineering fluorinase enzymes for synthesis of fluorophenyl compounds
发明人：R PRAVIN KUMAR; G GLADSTONE SIGAMANI; L ROOPA; B K NAVEEN; SHETTY ANUJ J; M LIKITH
权利人:KCAT ENZYMATIC PRIVATE LTD
摘要:The present invention discloses a computer-implemented method for engineering fluorinase enzymes towards the synthesis of fluorophenyl compounds. Limited or no mechanistic details of fluorinase enzymes have hindered progress in understanding their catalytic mechanisms for synthesizing synthetic organofluorine compounds. Through a comprehensive computational screening process, specific methionine-sulfonium phenyl substrates, including [(3S)-3-amino-3-carboxypropyl][2,5-difluoro-4-(4-methoxy-2,4-dioxobutyl)phenyl]methylsulfonium, were designed and optimized using quantum chemical optimization techniques. This methodology uncovers crucial information on F— ion attack conformation and the catalytic mechanism of the substrate, leading to the formation of Methyl 3-oxo-4-(2,4,5-trifluorophenyl)butanoate. Furthermore, a protein sequence and 3D modeling-based enzyme screening process was employed to identify the most suitable enzyme for this substrate. The identified enzyme was then engineered using the mechanistic insights gained from the studies, resulting in improved substrate scope, stability and catalytic efficiency. This computer-based approach offers an efficient and precise alternative to traditional trial-and-error methods, advancing the field towards the successful synthesis fluorophenyl compounds.