专利号:US-2012190064-A1 优先权日:2004-10-01 标题 :Feeding buffers, systems, and methods for in vitro synthesis of biomolecules 发明人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA 权利人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA; LIFE TECHNOLOGIES CORP 摘要:Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of a feeding solution that includes amino acids and an energy source to an ongoing in vitro synthesis system. The invention also includes methods of using a high-yield in vitro synthesis system to produce large quantities of proteins with incorporated labeled amino acids for analysis by methods such as by NMR. The invention further includes vectors for enhanced production of proteins from nucleic acid templates using in vitro synthesis systems.
专利号:US-6767723-B2 优先权日:2000-04-27 标题 :Method for synthesis of nucleic acids 发明人:TONOIKE HIROSHI 权利人:SHIMADZU CORP 摘要:An object of the present invention is to provide a method of treatment and a method of storage that are useful in conducting a nucleic acid synthesis procedure capable of directly amplifying an intended nucleic acid in a living body-derived sample without purification steps.The present invention provides a method for synthesis of nucleic acids in which a living body-derived sample itself is mixed with a reaction solution for gene amplification and allowed to react, which method comprises treating the sample with a surfactant before the reaction to destruct solid components such as cells or bacterial bodies containing nucleic acids and uniformly disperse them in the sample liquid.
专利号:US-2006014271-A1 优先权日:2004-07-16 标 题 :Fabrication of a completely polymeric microfluidic reactor for chemical synthesis 发明人:SONG YUJUN; KUMAR CHALLA; HORMES JOSEF 权利人:SONG YUJUN; KUMAR CHALLA; HORMES JOSEF 摘要:An inexpensive apparatus and method of fabricating completely polymeric (e.g., SU-8, PMMA, and PEEK) microfluidic reactors suitable for the synthesis of chemicals, particularly nanoparticles (e.g., mono, bi, tri, alloy, core-shell, polymeric, and metal-polymer nano-particles), is disclosed. A high precision process uses polymeric microfluidic patterning techniques and a new microfluidic sealing technique, referred to as “flexible semi-solid transfer,â€? to fabricate high aspect ratio polymeric micro-reactors. In one embodiment, high quality microfluidic channels are patterned onto a support substrate. The microfluidic structure is then sealed by transferring a polymeric material from a sacrificial substrate to the microfluidic structure, and cured. Then, the structure is bonded to a second support structure to form a micro-reactor.
专利号:US-8541524-B2 优先权日:2006-07-04 标 题 :Free-radical polymerization process in aqueous dispersion for the preparation of a block copolymer at least one block of which is a block of a halogenated polymer 发明人:FRINGANT CHRISTOPHE; VANDERVEKEN YVES; LACROIX-DESMAZES PATRICK; TONNAR JEFF 权利人:FRINGANT CHRISTOPHE; VANDERVEKEN YVES; LACROIX-DESMAZES PATRICK; TONNAR JEFF; SOLVAY 摘要:Process of controlled free-radical polymerization in aqueous dispersion for the preparation of a block copolymer at least one block of which is a block of a halogenated polymer using molecular iodine and at least one oxidant whose solubility in water is at least 10 g/l for the synthesis of the first block of the block copolymer.
专利号:BR-102019028281-A2 优先权日:2019-12-30 标 题 :METHOD OF SYNTHESIS OF NANOPARTICLES WITH BIOMEDICAL APPLICATION INCLUDED BY A NUCLEUS AND DOUBLE-FUNCTIONALIZED SURFACE WITH COATING OF BIOLOGICAL MOLECULES OF INTEREST
专利号:WO-2009143405-A2 优先权日:2008-05-22 标 题:Synthesis of graphene sheets and nanoparticle composites comprising same
[参考文献]: Abhay H Pande, Et Al. Modulation Of Human 5-Lipoxygenase Activity By Membrane Lipids. Biochemistry. 2004 Nov 23;43(46):14653-66. [参考文献]: B Fricke, Et Al. Quantitative Determination Of Zwitterionic Detergents Using Salt-Induced Phase Separation Of Triton X-100. Anal Biochem. 2000 Jun 1;281(2):144-50. [参考文献]: H L Liou, Et Al. Role Of Surface Lysine Residues Of Adipocyte Fatty Acid-Binding Protein In Fatty Acid Transfer To Phospholipid Vesicles. Biochemistry. 2001 May 29;40(21):6475-85. [参考文献]: J W Anderson, Et Al. Dipeptide Binding To The Extended Active Site Of The Streptomyces R61 D-Alanyl-D-Alanine-Peptidase: The Path To A Specific Substrate. Biochemistry. 2000 Oct 10;39(40):12200-9. [参考文献]: María Muoz, Et Al. Conductometric, Surface Tension, And Kinetic Studies In Mixed Sds-Tween 20 And Sds-Sb3-12 Micellar Solutions. Langmuir. 2004 Dec 7;20(25):10858-67.
合成参考文献
摘要:S13 | EUCOSMETICS | Combined Inventory of Ingredients Employed in Cosmetic Products (2000) and Revised Inventory (2006) | DOI:10.5281/zenodo.2624118 摘要:International Journal of Toxicology 43(Suppl. 1):30S-49S, 2024 参考文献:10.1134/s0006297919060105 摘要:Khomenko VA, Sidorin EV, Bakholdina SI, Naberezhnykh GA, Kim NY, Stenkova AM, Chernysheva NY, Isaeva MP, Solov'eva TF. Inclusion Bodies of Recombinant OmpF Porin from Yersinia pseudotuberculosis: Properties and Structural Characterization. Biochemistry (Mosc). 2019 Jun;84(6):672–85. doi: 10.1134/s0006297919060105.