CAS: 20244-86-4; 7-Methylguanosine

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CAS号118-00-3 鸟苷 | CAS号77-78-1 硫酸二甲酯 | CAS号74-88-4 碘甲烷 | CAS号578-76-7 7-甲基鸟嘌呤 | CAS号18646-11-2 α-D-ribofuranos...

7-Methylguanosine 5'-Monophosphate置于human Cytoplasmic Nucleotidase Iii B,Magnesium Chloride体系中,用 Aq. Buffer 用作溶剂,化学反应生成7-甲基鸟苷
参考文献:具有5'-(1,2,3-三唑基)部分的7-甲基鸟苷单磷酸酯类似物:合成和评估cniiib核苷酸酶的抑制剂
标题:具有5'-(1,2,3-三唑基)部分的7-甲基鸟苷单磷酸酯类似物:合成和评估cniiib核苷酸酶的抑制剂
摘要:5'-核苷酸酶催化核苷5'-单磷酸酯水解为相应的核苷和无机磷酸酯,从而有助于控制内源性核苷酸转换,并影响细胞中外源性递送核苷酸和核苷衍生的治疗药物的命运.最近鉴定出的核苷酸酶cniiib显示偏爱7-甲基鸟苷一磷酸(m 7 Gmp)作为底物,这表明其可能参与mrna降解.但是,生物学功能的程度和cniiib的意义仍有待阐明.在这里,我们合成了一系列的m 7在5'位置带有1,2,3-三唑部分的gmp类似物作为人类cniiib的潜在抑制剂.这些化合物是通过在5'-叠氮基5'-脱氧-7-甲基鸟苷与带有末端炔烃的不同磷酸盐或膦酸酯衍生物之间使用铜催化的叠氮化物-炔烃环加成(cuaac)合成的.使用HPLC和孔雀石绿分析法评估了类似物作为cniiib抑制剂,表明带有1,2,3-三唑酰基膦酸酯部分的化合物1A在微摩尔浓度(ic 50 87.8+/-7.5 µm)下抑制cniiib活性,而其他类似物未显示活动.此外,化合物1D被鉴定为hs
Doi:10.1016/j.Bmc.2017.11.032

专利信息

专利号:US-6214987-B1
优先权日:1994-09-02
标 题 :Compositions for enzyme catalyzed template-independent formation of phosphodiester bonds using protected nucleotides
发明人：HIATT ANDREW C; ROSE FLOYD
权利人:HIATT ANDREW C; ROSE FLOYD D
摘要:A method for the stepwise creation of phosphodiester bonds between desired nucleosides resulting in the synthesis of polynucleotides having a predetermined nucleotide sequence by preparing an initiation substrate containing a free and unmodified 3'-hydroxyl group; attaching a mononucleotide selected according to the order of the predetermined nucleotide sequence to the 3'-hydroxyl of the initiating substrate in a solution containing a catalytic amount of an enzyme capable of catalyzing the 5' to 3' phosphodiester linkage of the 5'-phosphate of the mononucleotide to the 3'-hydroxyl of the initiating substrate, wherein the mononucleotide contains a protected 3'-hydroxyl group, whereby the protected mononucleotide is covalently linked to the initiating substrate and further additions are hindered by the 3'-hydroxyl protecting group. Methods in which a mononucleotide immobilized on a solid support is added to a free polynucleotide chain are also disclosed.

专利号:US-5872244-A
优先权日:1994-09-02
标 题:3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
发明人：HIATT ANDREW C; ROSE FLOYD
权利人:HIATT ANDREW C; ROSE FLOYD D
摘要:A method for the stepwise creation of phosphodiester bonds between desired nucleosides resulting in the synthesis of polynucleotides having a predetermined nucleotide sequence by preparing an initiation substrate containing a free and unmodified 3'-hydroxyl group; attaching a mononucleotide selected according to the order of the predetermined nucleotide sequence to the 3'-hydroxyl of the initiating substrate in a solution containing a catalytic amount of an enzyme capable of catalyzing the 5' to 3' phosphodiester linkage of the 5'-phosphate of the mononucleotide to the 3'-hydroxyl of the initiating substrate, wherein the mononucleotide contains a protected 3'-hydroxyl group, whereby the protected mononucleotide is covalently linked to the initiating substrate and further additions are hindered by the 3'-hydroxyl protecting group. Methods in which a mononucleotide immobilized on a solid support is added to a free polynucleotide chain are also disclosed.

专利号:WO-2009026148-A1
优先权日:2007-08-17
标题:Selective 5' ligation tagging of rna
发明人：VAIDYANATHAN RAMESH; JENDRISAK JEROME J
权利人:EPICT TECHNOLOGIES CORP; VAIDYANATHAN RAMESH; JENDRISAK JEROME J
摘要:The present invention provides compositions, kits and methods for selective enrichment, isolation, purification, production, tagging, cloning, amplification, detection, quantification, characterization, and assay of nucleic acid molecules that either have a monophosphate group, a polyphosphate group, or a cap nucleotide on their 5' terminus. The resulting 5'-monophosphorylated RNA molecules generated using the method are 5' ligation tagged by ligating an RNA acceptor oligonucleotide to their 5' ends using RNA ligase. The tagged RNA can be used for full-length-selected synthesis of first-stand cDNA, or double-stranded cDNA for a variety of uses and applications.

专利号:US-5763594-A
优先权日:1994-09-02
标 题:3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
发明人：HIATT ANDREW C; ROSE FLOYD
权利人:HIATT ANDREW C; ROSE FLOYD D
摘要:A method for the stepwise creation of phosphodiester bonds between desired nucleosides resulting in the synthesis of polynucleotides having a predetermined nucleotide sequence by preparing an initiation substrate containing a free and unmodified 3'-hydroxyl group; attaching a mononucleotide selected according to the order of the predetermined nucleotide sequence to the 3'-hydroxyl of the initiating substrate in a solution containing a catalytic amount of an enzyme capable of catalyzing the 5' to 3' phosphodiester linkage of the 5'-phosphate of the mononucleotide to the 3'-hydroxyl of the initiating substrate, wherein the mononucleotide contains a protected 3'-hydroxyl group, whereby the protected mononucleotide is covalently linked to the initiating substrate and further additions are hindered by the 3'-hydroxyl protecting group. Methods in which a mononucleotide immobilized on a solid support is added to a free polynucleotide chain are also disclosed.

专利号:US-6232465-B1
优先权日:1994-09-02
标题 :Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides
发明人：HIATT ANDREW C; ROSE FLOYD
权利人:HIATT ANDREW C; ROSE FLOYD D
摘要:A method for the stepwise creation of phosphodiester bonds between desired nucleosides resulting in the synthesis of polynucleotides having a predetermined nucleotide sequence by preparing an initiation substrate containing a free and unmodified 3'-hydroxyl group; attaching a mononucleotide selected according to the order of the predetermined nucleotide sequence to the 3'-hydroxyl of the initiating substrate in a solution containing a catalytic amount of an enzyme capable of catalyzing the 5' to 3' phosphodiester linkage of the 5'-phosphate of the mononucleotide to the 3'-hydroxyl of the initiating substrate, wherein the mononucleotide contains a protected 3'-hydroxyl group, whereby the protected mononucleotide is covalently linked to the initiating substrate and further additions are hindered by the 3'-hydroxyl protecting group. Methods in which a mononucleotide immobilized on a solid support is added to a free polynucleotide chain are also disclosed.

专利号:US-5808045-A
优先权日:1994-09-02
标 题 :Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides
发明人：HIATT ANDREW C; ROSE FLOYD
权利人:HIATT ANDREW C; ROSE FLOYD D
摘要:A method for the stepwise creation of phosphodiester bonds between desired nucleosides resulting in the synthesis of polynucleotides having a predetermined nucleotide sequence by preparing an initiation substrate containing a free and unmodified 3'-hydroxyl group; attaching a mononucleotide selected according to the order of the predetermined nucleotide sequence to the 3'-hydroxyl of the initiating substrate in a solution containing a catalytic amount of an enzyme capable of catalyzing the 5' to 3' phosphodiester linkage of the 5'-phosphate of the mononucleotide to the 3'-hydroxyl of the initiating substrate, wherein the mononucleotide contains a protected 3'-hydroxyl group, whereby the protected mononucleotide is covalently linked to the initiating substrate and further additions are hindered by the 3'-hydroxyl protecting group. Methods in which a mononucleotide immobilized on a solid support is added to a free polynucleotide chain are also disclosed.