CAS: 12279-41-3; Seractide

专利信息

专利号:US-11419919-B1
优先权日:2021-04-12
标题 :High-purity adrenocorticotropic hormone, analogue and a large-scale preparation method thereof
发明人：MENG JUNDONG; Rui Kangning; LIU BIN; HAN YUANYUAN; CHEN SONG; ZHANG HAONING
权利人:NANJING HANXIN PHARMACEUTICAL TECH CO LTD
摘要:The invention belongs to the technical field of polypeptide preparation methods, and in particular relates to a high-purity ACTH (human sequence) or analogue and large-scale preparation method thereof. The main steps include: amino acids are coupled from the C-terminal to the N-terminal by Fmoc solid-phase synthesis method to obtain the crude ACTH (human sequence) or analogue peptidyl-resin with protective groups, wherein the reaction temperature of C-15 peptide synthesis is 40-60° C. After cleavge and precipitation, the crude product of ACTH (human sequence) or analogue is obtained, and then the high-purity product is obtained by liquid chromatography. The chromatographic purity of ACTH (human sequence) or analogue prepared by the invention is more than 99%, the stability is good, and the yield of the target peptide is ≥63%.