CAS: 781658-23-9; 4-Hydroxyisoleucine

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CAS号28664-35-9 4,5-二甲基-3-羟基-2,... | CAS号99506-67-9 3-hydroxy-2-met...

L-异亮氨酸置于ferrous(II) Sulfate Heptahydrate,维生素 C体系中,用 Aq. Buffer 作为反应溶剂,化学反应生成 4-羟基-L-异亮氨酸
参考文献:L-异亮氨酸双加氧酶的半理性设计产生其芳香氨基酸羟基化活性
标题:L-异亮氨酸双加氧酶的半理性设计产生其芳香氨基酸羟基化活性
摘要:Fe (II)-和 2-酮戊二酸依赖性双加氧酶 (Fe (II)/α-Kg Dos) 已被应用于催化氨基酸的羟基化.然而,已经开发和表征的 Fe (II)/α-Kg Dos 还不够. L-异亮氨酸双加氧酶 (Ido) 是一种 Fe (II)/α-Kg Do,特异性催化 L-异亮氨酸 (L-Ile) 形成 4-羟基异亮氨酸 (4-Hil),并对 L-脂肪族化合物表现出底物特异性氨基酸.为了将ido的底物谱扩展到芳香族氨基酸,本研究利用分子动力学(md)模拟分析了ido的底物谱的规律性,发现α-Kg的fe2+,C2与氨基酸链的距离c4 对于调节酶的底物特异性可能至关重要.突变位点(y143,S153 和 R227)也根据极性口袋和残基自由能贡献进行单点饱和突变.发现y143D,Y143I和s153A突变体表现出催化l-苯丙氨酸活性,而y143I,S153A,S153Q和s153Y表现出催化l-
DOI:10.3390/molecules28093750

专利信息

专利号:US-7670822-B2
优先权日:2006-03-24
标题 :Aldolase and production process of 4-hydroxy-L-isoleucine
发明人：SMIRNOV SERGEY VASILIEVICH; SAMSONOVA NATALIA NIKOLAEVNA; KODERA TOMOHIRO; MATSUI KAZUHIKO
权利人:AJINOMOTO KK
摘要:A novel aldolase is described. 4-hydroxy-3-methyl-2-keto-pentanoic acid, which is useful as an intermediate in the synthesis of 4-hydroxy-L-isoleucine, may be synthesized from acetaldehyde and α-ketobutyric acid using a novel aldolase, which is derived from the genus Arthrobacter.

专利号:US-2012282652-A1
优先权日:2011-02-28
标题 :Preparation of peptide mixtures by protease catalysis designed to provide useful biological and physical properties
发明人：GROSS RICHARD A; VISWANATHAN KODANDARAMAN; QIN XU
权利人:GROSS RICHARD A; VISWANATHAN KODANDARAMAN; QIN XU; POLITECHNIC INST UNIV NEW YORK
摘要:A process for preparing unique peptide mixtures with a broad range of uses using combinations of natural and non-natural amino acid alkyl ester monomers and specific combinations of these monomers as dimers, trimers and higher oligomers selected from the large group of structural motifs that lead to useful physical and/or biological properties and that have been or may be identified from solid state peptide synthesis, isolation of peptides from natural sources, and production of peptides by recombinant DNA methods, or identification of peptides by recombinant methods such as phage display, the method of peptide synthesis comprising a) admixing one or more natural and non-natural amino acid alkyl ester monomer, dimer, trimer and higher oligomers with one or more proteases in a reaction medium; b) heating the mixture to between about 5° C. to about 90° C. for between 5 minutes and 24 hours; and c) recovering the formed oligopeptide.