CAS: 1109-28-0; (2R,3R,4R,5R)-4-(((2R,3R,4R,5S,6R)-3,4-Dihydroxy-6-(Hydroxymethyl)-5-(((2R,3R,4S,5S,6R)-3,4,5-Trihydroxy-6-(Hydroxymethyl)Tetrahydro-2H-Pyran-2-yl)Oxy)Tetrahydro-2H-Pyran-2-yl)Oxy)-2,3,5,6-Tetrahydroxyhexanal

上下游产品

Sucrose D-maltose β-cyclodextrin D-maltohexaoseD-glucose 45H63NO19SC45H63NO19S 44H65N5O20C44H65N5O20 maltotriose peracetate

蔗糖置于水,三羟甲基氨基甲烷盐酸盐体系中,化学反应 45.0H,反应生成麦芽三糖
参考文献:Molecular Cloning And Functional Expression Of A New Amylosucrase Fromalteromonas Macleodii
标题:Molecular Cloning And Functional Expression Of A New Amylosucrase Fromalteromonas Macleodii
摘要:研究人员对12种alteromonas和pseudoalteromonas菌株中的淀粉糖苷酶进行了检测.两种alteromonas菌株(alteromonas Addita Kctc 12195和alteromonas Macleodii Kctc 2957)的基因与已知的淀粉糖苷酶具有高度同源性.研究人员从a. Addita和a. Macleodii中获得了含有淀粉糖苷酶类似物的基因组克隆,并推断出相应基因(分别为aaas和amas)的氨基酸序列,发现它们与多糖奈瑟菌(neisseria Polysaccharea),辐射嗜热菌(deinococcus Radiodurans)和地热嗜热菌(deinococcus Geothermalis)的淀粉糖苷酶高度相似.在大肠杆菌中成功表达amas的功能,并在纯化的重组amas中检测到典型的淀粉糖苷酶活性,而纯化的重组aaas则没有活性.虽然amas的最大总活性在45oc时观测到,但转糖苷化与总活性的比率随着温度从55oc降低到25oc而增加.这些结果表明,转糖苷化在较低温度下优先发生,而水解在较高温度下占主导地位.
Doi:10.1271/bbb.80891

专利信息

专利号:US-9034844-B2
优先权日:2009-04-06
标 题 :6′-sialyllactose salts and process for their synthesis and for the synthesis of other alpha-sialyloligosaccharides
发明人：TAMERLANI GIANCARLO; LOMBARDI ILARIA; BARTALUCCI DEBORA; DANESI ANDREA; SALSINI LIANA; MANONI MARCO; CIPOLLETTI GIOVANNI
权利人:TAMERLANI GIANCARLO; LOMBARDI ILARIA; BARTALUCCI DEBORA; DANESI ANDREA; SALSINI LIANA; MANONI MARCO; CIPOLLETTI GIOVANNI; INALCO SPA
摘要:The present invention relates to a process of synthesis of α-sialyl oligosaccharides and in particular of 6′-sialyllactose and its salts comprising a step of coupling by Koenigs-Knorr reaction under conditions that allow its use on an industrial scale.

专利号:US-10973847-B2
优先权日:2017-06-30
标题 :Core-to-surface polymerization for the synthesis of star polymers and uses thereof
发明人：JOHNSON JEREMIAH A; GOLDER MATTHEW R
权利人:MASSACHUSETTS INST TECHNOLOGY
摘要:Disclosed are methods, compositions, reagents, systems, and kits to prepare star polymers, as well as compositions and uses thereof. Various embodiments show that synthesis of these polymers contain low metal concentration to provide polymers for diverse biomedical applications including in vivo applications.

专利号:WO-2024165611-A1
优先权日:2023-02-07
标题:Production of a disaccharide and/or milk oligosaccharide by a cell with reduced synthesis of udp-glcnac
发明人：BEAUPREZ JOERI; DECOENE THOMAS; VERCAUTEREN ANNELIES
权利人:INBIOSE NV
摘要:The present invention is in the technical field of synthetic biology, metabolic engineering and cell cultivation. The invention provides a cell for production of a disaccharide and/or an oligosaccharide like a milk oligosaccharide wherein synthesis of UDP-N-acetylglucosamine in said cell is rendered less functional. The invention further provides use of said cell in a cultivation or incubation. The invention also describes methods for the production of a disaccharide and/or an oligosaccharide like a milk oligosaccharide using said cell as well as the purification of said disaccharide and/or oligosaccharide like a milk oligosaccharide.

专利号:WO-2024165524-A1
优先权日:2023-02-06
标 题:Production of a saccharide by a cell with reduced synthesis of lactobionic acid
发明人：BEAUPREZ JOERI; DECOENE THOMAS; VERCAUTEREN ANNELIES
权利人:INBIOSE NV
摘要:The present invention is in the technical field of synthetic biology, metabolic engineering and cell cultivation. The invention provides a cell for production of a saccharide wherein synthesis of lactobionic acid in said cell is rendered less functional or is knocked out. The invention further provides use of said cell in a cultivation or incubation. The invention also describes methods for the production of a saccharide using said cell as well as the purification of said saccharide.

专利号:US-12378589-B2
优先权日:2016-12-27
标 题 :In vivo synthesis of sialylated compounds
发明人：BEAUPREZ JOERI; COUSSEMENT PIETER; VAN HERPE DRIES; PETERS GERT; VERCAUTEREN ANNELIES
权利人:INBIOSE NV
摘要:This disclosure is in the technical field of synthetic biology and metabolic engineering. More particularly, this disclosure is in the technical field of fermentation of metabolically engineered microorganisms. This disclosure describes engineered micro-organisms able to synthesize sialylated compounds via an intracellular biosynthesis route. These micro-organisms can dephosphorylate N-acetylglucosamine-6-phosphate to N-acetyl glucosamine and convert the N-acetylglucosamine to N-acetylmannosamine. These micro-organisms also have the ability to convert N-acetylmannosamine to N-acetyl-neuraminate. Furthermore, this disclosure provides a method for the large scale in vivo synthesis of sialylated compounds, by culturing a microorganism in a culture medium, optionally comprising an exogenous precursor such as, but not limited to lactose, lactoNbiose, N-acetyllactosamine and/or an aglycon, wherein the microorganism intracellularly dephosphorylates N-acetylglucosamine-6-phosphate to N-acetylglucosamine, converts N-acetyl-glucosamine to N-acetylmannosamine and convert the latter further to N-acetyl-neuraminate.

专利号:WO-2023118363-A1
优先权日:2021-12-22
标题:Method for the synthesis of a carbonyl-functionalised adduct
发明人：GODEHARD SIMON P; PELZER ALEXANDER
权利人:BRAIN Biotech AG
摘要:The invention relates to a method for the synthesis of a carbonyl-functionalised adduct.