CAS: 2818-58-8; Phenyl B-D-Galactoside

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CAS号10257-28-0 D-吡喃葡萄糖 | CAS号644-76-8 1,6-内醚-D-半乳糖

1,2,3,4,6-D-葡萄糖五乙酸酯置于甲醇,Sodium Hydroxide,氢溴酸,Sodium Methylate,四丁基硫酸氢铵,溶剂黄146体系中,用 二氯甲烷,水 用作溶剂,化学反应生成苯基-Beta-D-吡喃半乳糖苷
参考文献:阐明肝素黄杆菌软骨素ac裂解酶裂解多糖的机制
标题:阐明肝素黄杆菌软骨素ac裂解酶裂解多糖的机制
摘要:来自肝素黄杆菌的软骨素 Ac 裂解酶通过消除机制降解硫酸软骨素糖胺聚糖,产生在其非还原端具有 Delta4,5-不饱和糖醛酸残基的二糖或寡糖.关于该酶促反应的键断裂和形成步骤的顺序的机制细节不存在,主要是由于聚合物底物的不均匀性质.为这种酶创建了一类新的合成底物,可以测量定义的和可重复的 K(Cat) 和 K(M) 值,并扩大了可以进行的机械研究的范围.主要氘动力学同位素对 K(Cat)/k(M) 的质子 α 提取到羧酸的影响测量为 1.67 +/-0.07,表明去质子化发生在限速步骤中.使用具有不同反应性的离去基团的底物,产生了平坦的线性自由能关系,表明 C4-O4 键在速率决定步骤中没有断裂.综上所述,这些结果强烈暗示了一种逐步机制.与此一致的是二次氘动力学同位素对 4-[(2)H]-基板上的 1.01 +/-0.03 的 K(Cat)/k(M) 影响的测量,表明在c4 在限速步骤
Doi:10.1021/ja020627C

专利信息

专利号:WO-2024165611-A1
优先权日:2023-02-07
标题:Production of a disaccharide and/or milk oligosaccharide by a cell with reduced synthesis of udp-glcnac
发明人：BEAUPREZ JOERI; DECOENE THOMAS; VERCAUTEREN ANNELIES
权利人:INBIOSE NV
摘要:The present invention is in the technical field of synthetic biology, metabolic engineering and cell cultivation. The invention provides a cell for production of a disaccharide and/or an oligosaccharide like a milk oligosaccharide wherein synthesis of UDP-N-acetylglucosamine in said cell is rendered less functional. The invention further provides use of said cell in a cultivation or incubation. The invention also describes methods for the production of a disaccharide and/or an oligosaccharide like a milk oligosaccharide using said cell as well as the purification of said disaccharide and/or oligosaccharide like a milk oligosaccharide.

专利号:WO-2024165524-A1
优先权日:2023-02-06
标 题:Production of a saccharide by a cell with reduced synthesis of lactobionic acid
发明人：BEAUPREZ JOERI; DECOENE THOMAS; VERCAUTEREN ANNELIES
权利人:INBIOSE NV
摘要:The present invention is in the technical field of synthetic biology, metabolic engineering and cell cultivation. The invention provides a cell for production of a saccharide wherein synthesis of lactobionic acid in said cell is rendered less functional or is knocked out. The invention further provides use of said cell in a cultivation or incubation. The invention also describes methods for the production of a saccharide using said cell as well as the purification of said saccharide.

专利号:US-5262312-A
优先权日:1990-04-25
标 题:Process for the glycosidase-catalyzed synthesis of glyco conjugates
发明人：HOLLA WOLFGANG; SCHUDOK MANFRED
权利人:HOECHST AG
摘要:It has been possible to show for a first time with the process according to the invention that the synthesis of glyco conjugates is possible by direct linkage of sugars with serine, serine derivatives and serine peptides with the aid of glucosidases.

专利号:US-7306938-B2
优先权日:1999-12-29
标 题:High throughput screen for inhibitors of fatty acid biosynthesis in bacteria
发明人：MURPHY CHRISTOPHER; YOUNGMAN PHILIP
权利人:MILLENNIUM PHARM INC
摘要:Methods for identifying compounds that are inhibitors of bacterial fatty acid biosynthesis are disclosed. Such compounds can be used as lead compounds in methods for preparing antibacterial agents for treating bacterial infections (e.g., in humans, animals, and plants). Inhibitors of bacterial fatty acid synthesis can also be tested for their ability to inhibit synthesis of acylated homoserine lactones. Compounds that inhibit synthesis of acylated homoserine lactones can be used as inhibitors of bacterial virulence. The disclosed methods allow for high throughput screening of libraries of test compounds.

专利号:US-6268193-B1
优先权日:1990-02-14
标题:Methods and products for the synthesis of oligosaccharide structures on glycoproteins, glycolipids, or as free molecules, and for the isolation of cloned genetic sequences that determine these structures
发明人：LOWE JOHN B
权利人:UNIV MICHIGAN
摘要:A method for isolating a gene, comprising: n (i) isolating a cell possessing a post-translational characteristic of interest, said post-translational characteristic being the presence of a membrane-bound oligosaccharide or polysaccharide of interest on the surface of said cell, the presence of a soluble oligosaccharide or polysaccharide of interest in an extract of said cell, or the presence of a particularly glycosyltransferase activity in an extract of said cell; n (ii) creating a genetic library of either cDNA or genomic DNA from the genetic material of said isolated cell; n (iii) transforming host cells with said genetic library; and n (iv) screening said transformed host cells for a host cell containing said post-translational characteristic, thereby obtaining a cell containing said gene, is disclosed. The method can be used to obtain genes encoding glycosyltransferases.

专利号:WO-2024165525-A1
优先权日:2023-02-06
标题:Production of a saccharide, lactobionic acid and/or glycosylated forms of lactobionic acid
发明人：BEAUPREZ JOERI; DECOENE THOMAS; VERCAUTEREN ANNELIES
权利人:INBIOSE NV
摘要:The present invention is in the technical field of synthetic biology, metabolic engineering and cell cultivation. The invention provides a cell for production of a saccharide with enhanced synthesis of lactobionic acid (LBA) and use of said cell in a cultivation or incubation. The invention also describes methods for the production of a saccharide or of a mixture of a saccharide and LBA using said cell as well as the purification of said saccharide or said mixture of said saccharide and LBA. The invention also provides a cell for production of a glycosylated form of LBA and use of said cell in a cultivation or incubation. Furthermore, the invention describes methods for the production of a glycosylated form of LBA using said cell, as well as for the production of a mixture comprising i) a glycosylated form of LBA and a saccharide or ii) a glycosylated form of LBA, a saccharide and LBA. The invention also describes purification of said glycosylated form of LBA or of a mixture comprising i) a glycosylated form of LBA and a saccharide or ii) a glycosylated form of LBA, a saccharide and LBA.