CAS: 69-89-6; 1H-Purine-2,6(3H,7H)-Dione

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CAS号73-40-5 鸟嘌呤 | CAS号2969-81-5 4-溴丁酸乙酯 | CAS号76194-10-0 1-butyl-5-formy... | CAS号69-93-2 尿酸 | CAS号6979-73-3 1-(4-chloro-phe... | CAS号5442-24-0 4-氨基-2,6-二羟基-5-亚硝基嘧啶 | CAS号77287-34-4 甲亚胺酸 | CAS号290-87-9 1,3,5-三嗪 | CAS号3240-72-0 5,6-氨基-2,4(1H,3... | CAS号146-80-5 黄嘌呤核苷 | CAS号20419-68-5 2,6-二氯-9-(四氢-2H... | CAS号83-67-0 可可碱 | CAS号58-08-2 咖啡因 | CAS号2002-59-7 6-硫代-2-羟基嘌呤 | CAS号97-59-6 尿囊素 | CAS号87-42-3 6-氯嘌呤 | CAS号5451-40-1 2,6-二氯嘌呤 | CAS号69-89-6 黄嘌呤 | CAS号69-93-2 尿酸 | CAS号61066-33-9 pyrimidine-2,4,...

尿酸置于cyclothionine,Xanthine Oxidase体系中,用 Phosphate Buffer 作为反应溶剂,化学反应生成 黄嘌呤
参考文献:Comprehensive Study Of Bioanalytical Platforms: Xanthine Oxidase
标题:Comprehensive Study Of Bioanalytical Platforms: Xanthine Oxidase
摘要:在开发广泛适用性方法的框架内,以黄嘌呤氧化酶(xnox)为案例,对生物传感器应用的通用生物分析平台进行了全面研究.在此背景下,重点放在基于 Xnox 的安培计生物传感器上,Xnox 通过共价结合固定在经二硫代双-N-琥珀酰亚胺丙酸酯修饰的金电极上.在液体条件下使用原子力显微镜和石英晶体微天平技术对固定 Xnox 层进行了表征.此外,还利用扫描电化学显微镜对酶活性进行了空间分辨绘图.研究发现,吩噻嗪衍生物的氧化还原染料,特别是亚硫酰和亚甲基蓝,可以很好地作为还原氧化锡的电子受体.计算了上述氧化还原染料存在时黄嘌呤酶促氧化的动力学参数和平衡常数.在溶液中有亚硫酸或亚甲蓝作为氧化还原媒介的情况下,酶电极对不同浓度黄嘌呤的反应也不同.在这些条件下,黄嘌呤可以在 +0.2 V 的电压下通过安培法测定.
DOI:10.1021/ac051676L

专利信息

专利号:US-5977301-A
优先权日:1992-09-24
标题 :Synthesis of N-substituted oligomers
发明人：ZUCKERMAN RONALD N; KERR JANICE M; KENT STEPHEN B H; MOOS WALTER H; SIMON REYNA J; GOFF DANE A
权利人:CHIRON CORP
摘要:A solid-phase method for the synthesis of N-substituted oligomers, such as poly (N-substituted glycines) (referred to herein as poly NSGs) is used to obtain oligomers, such as poly NSGs of potential therapeutic interest which poly NSGs can have a wide variety of side-chain substituents. Each N-substituted glycine monomer is assembled from two 'sub-monomers' directly on the solid support. Each cycle of monomer addition consists of two steps: (1) acylation of a secondary amine bound to the support with an acylating agent comprising a leaving group capable of nucleophilic displacement by -NH2, such as a haloacetic acid, and (2) introduction of the side-chain by nucleophilic displacement of the leaving group, such as halogen (as a resin-bound alpha -haloacetamide) with a sufficient amount of a second sub-monomer comprising an -NH2 group, such as a primary amine, alkoxyamine, semicarbazide, acyl hydrazide, carbazate or the like. Repetition of the two step cycle of acylation and displacement gives the desired oligomers. The efficient synthesis of a wide variety of oligomeric NSGs using automated synthesis technology of the present method makes these oligomers attractive candidates for the generation and rapid screening of diverse peptidomimetic libraries. The oligomers of the invention, such as N-substituted glycines (i.e. poly NSGs) disclosed here provide a new class of peptide-like compounds not found in nature, but which are synthetically accessible and have been shown to possess significant biological activity and proteolytic stability.

专利号:EP-0671928-B1
优先权日:1992-09-24
标题 :Synthesis of n-substituted oligomers
发明人：ZUCKERMANN RONALD N; KERR JANICE M; KENT STEPHEN BRIAN HENRY; MOOS WALTER H; SIMON REYNA J; GOFF DANE A
权利人:CHIRON CORP
摘要:Poly N-substituted Glycines (poly NSGs), wherein the substituents bear purine or pyrimidine bases (R<9>) every second glycine: In addition, a solid phase method for the synthesis of N-substituted oligomers of more general structures is disclosed.The poly NSGs obtainable by this method can have a wide variety of side-chain substituents. Each N-substituted glycine monomer is assembled from two 'sub-monomers' directly on the solid support. Each cycle of monomer addition consists of two steps: (1) acylation of a secondary amine bound to the support with an acylating agent comprising a leaving group capable of nucleophilic displacement by -NH2, such as a haloacetic acid, and (2) introduction of the side-chain by nucleophilic displacement of the leaving group, such as halogen (as a resin-bound alpha -haloacetamide) with a sufficient amount of a second sub-monomer comprising an -NH2 group, such as a primary amine, alkoxyamine, semicarbazide, acyl hydrazide, carbazate or the like. Repetition of the two step cycle of acylation and displacement gives the desired oligomers. The efficient synthesis of a wide variety of oligomeric NSGs using the automated synthesis technology of the present method makes these oligomers attractive candidates for the generation and rapid screening of diverse peptidomimetic libraries. The oligomers of the invention, such as N-substituted glycines (i.e. poly NSGs) disclosed here provide a new class of peptide-like compounds not found in nature, but which are synthetically accessible and have been shown to possess significant biological activity and proteolytic stability.

专利号:US-2004152905-A1
优先权日:2003-01-31
标题:Universal building blocks and support media for synthesis of oligonucleotides and their analogs
发明人：GUZAEV ANDREI P; MANOHARAN MUTHIAH
摘要:Compounds for the synthesis of oligomeric compounds, particularly oligonucleotides and chemically modified oligonucleotide analogs, are provided. In addition, methods for functionalization of a support medium with a first monomeric subunit and methods for the synthesis of oligomeric compounds utilizing the novel compounds bound to support media are provided.

专利号:US-2004242897-A1
优先权日:2002-07-31
标题 :Universal support media for synthesis of oligomeric compounds
发明人：GUZAEV ANDREI P; MANOHARAN MUTHIAH
摘要:Compounds for the synthesis of oligomeric compounds, particularly oligonucleotides and oligonucleotide mimetics, are provided. In addition, methods for functionalizing a support medium with a first monomeric subunit and methods for the synthesis of oligomeric compounds utilizing the novel compounds bound to support media are provided.

专利号:US-6515039-B1
优先权日:1998-08-28
标 题:Method for the parallel and combinatory synthesis of compounds bound to a continuous polymeric solid phase supporting material
发明人：ULBRICHT MATHIAS; VOLKMER-ENGERT RUDOLF; GERMEROTH LOTHAR; WENSCHUH HOLGER
权利人:POLY AN GMBH
摘要:The invention relates to a method for the parallel and combinatory synthesis of compounds bound to a continuous polymeric solid phase supporting material. According to the method, a continuous polymeric solid phase supporting material is prepared, the material comprising a matrix of a supporting polymeric material and graft copolymer chains covalently bound to the supporting matrix, the graft copolymer chains having reactive groups being able to react with organic compounds, thereby forming spatially defined reaction sites. The method then comprises the sequential spotting of synthesis building blocks or reagents at the various reaction sites on the continuous solid phase supporting material to yield a substrate library of compounds bound to the solid phase supporting material, whereby each reaction site on the continuous solid phase supporting material determines the composition of the synthesized compound, which is bound directly on the supporting matrix via the reactive groups of the graft polymer chain.

专利号:US-5789577-A
优先权日:1994-12-30
标 题 :Method for the controlled synthesis of polynucleotide mixtures which encode desired mixtures of peptides
发明人：GEYSEN H MARIO
权利人:CHIRON CORP
摘要:A method to obtain selected individual polynucleotides or mixtures thereof each of which encodes a peptide and at least one polynucleotide of the mixture encodes a peptide having a target property. The polynucleotides of the invention present in the mixture in detectable, retrievable, and clonable amounts are expressed in a host organism for screening for the target activity. The invention features the ability to synthesize controlled random polynucleotides to produce a predetermined mixture of polynucleotides and to avoid synthesis of a stop codon by adjusting the proportions into which the synthesis pool is subdivided and by adjusting the proportions of activated nucleotides added at each coupling step. A polynucleotide encoding a peptide having a target property can be selected and sequenced to deduce the amino acid sequence of the peptide.