专利号:US-9612244-B2 优先权日:2007-06-22 标题:Fluorescent labeling of transfer RNA and study of protein synthesis 发明人:COOPERMAN BARRY S; SMILANSKY ZEEV; GOLDMAN YALE E; PAN DONGLI 权利人:UNIV PENNSYLVANIA; ANIMA CELL METROLOGY INC 摘要:Provided are methods for labeling transfer RNA comprising replacing the uracil component of a dihydrouridine of said transfer RNA with a fluorophore. The disclosed methods may comprise fluorescent labeling of natural tRNAs (i.e., tRNAs that have been synthesized in a cell, for example, in a bacterium, a yeast cell, or a vertebrate cell) at dihydrouridine (D) positions, or fluorescent labeling of synthetic tRNAs. In another aspect, the present invention provides methods for assessing protein synthesis in a translation system comprise providing a tRNA having a fluorophore substitution for the uracil component of a dihydrouridine in a D loop of the tRNA; introducing the labeled tRNA into the translation system; irradiating the translation system with electromagnetic radiation, thereby generating a fluorescence signal from the fluorophore; detecting the fluorescence signal; and, correlating the fluorescence signal to one or more characteristics of the protein synthesis in the translation system. The disclosed methods are useful in single molecule as well as in ensemble settings.
专利号:US-4509970-A 优先权日:1981-12-17 标 题 :Substituted phenylphosphinyloxy- and phosphinylthio-iminocarboxylates useful for the control of weeds 发明人:LEE SHY-FUH 权利人:ZOECON CORP 摘要:Novel substituted phenylphosphinyloxy- and phenylphosphinylthio-iminocarboxylates, synthesis thereof, intermediates therefor, and the use of said novel compounds for the control of weeds.
专利号:WO-2022148811-A1 优先权日:2021-01-08 标 题 :Pharmaceutical composition containing a parp inhibitor and/or a genotoxic substance that intervenes in the synthesis phase (s-phase) of cell replication, combined with an inhibitor of the recq5 signal path 发明人:LÖBRICH MARKUS 权利人:UNIV DARMSTADT TECH 摘要:The present invention relates to new pharmaceutical compositions containing RECQ5 inhibitors for use in the treatment and/or prevention of homologous recombination (HR) proficient tumors and/or ATRX deficient tumors and/or ALT tumors in humans.
专利号:US-10921326-B2 优先权日:2007-06-22 标 题:Fluorescent labeling of transfer RNA and study of protein synthesis
专利号:CN-110156701-B 优先权日:2019-06-25 标题 :Synthesis method of 2,3,5, 6-tetramethylpyrazine
专利号:US-2014329234-A1 优先权日:2007-06-22 标 题 :Fluorescent labeling of transfer rna and study of protein synthesis
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合成参考文献
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