CAS: 24259-59-4; (2S,3S,4S)-2,3,4,5-Tetrahydroxypentanal

上下游产品

CAS号5328-37-0 L-阿拉伯糖 | CAS号4099-88-1 2,3-异亚丙氧基-D-呋喃核糖苷 | CAS号110773-08-5 (2S,3S,4S)-2,3,... | CAS号110744-96-2 2,3,4,5-tetrabe... | CAS号488-81-3 侧金盏花醇 | CAS号78008-36-3 (R)-1,4-二氧杂螺[4,... | CAS号93-59-4 过氧化氢苯甲酰 | CAS号67-66-3 氯仿 | CAS号152202-52-3 1,5-anhydro-2-d... | CAS号67-56-1 甲醇 | CAS号3080-30-6 1-O-乙酰基-2,3,5-O... | CAS号488-81-3 侧金盏花醇 | CAS号40733-11-7 L-Arabinose, bi...

Adonitol置于maltose Binding Protein/apium Graveolens Mannitol-1-Dehydrogenase Wild Type Fusion Protein,β-烟酰胺腺嘌呤二核苷酸,三羟甲基氨基甲烷体系中,用 水 用作溶剂,化学反应生成L-核糖
参考文献:Directed Evolution Toward Improved Production Of L-Ribose From Ribitol
标题:Directed Evolution Toward Improved Production Of L-Ribose From Ribitol
摘要:本文描述了利用重组大肠杆菌一步生产l-核糖的方法的改进.先前已对来自香芹apium Graveolens的甘露醇-1-脱氢酶(mdh)基因进行密码子优化,克隆到组成型pzuc10载体中,并在大肠杆菌中表达.此mdh催化甘露醇到d-甘露糖的nad依赖性转化,并能够将多种多元醇转化为相应的l-糖,包括将核糖醇转化为l-核糖.在此,通过易错pcr生成的文库和二硝基水杨酸试剂筛选,进行了三轮定向进化.选择对l-核糖转化率提高的突变体,并通过结合两次第二轮的突变分离出最佳突变体.文库还针对热稳定性进行了选择,并在每轮诱变中以不断提高的温度进行筛选.整体观测到19.2倍的改进,最终转化率为46.6 +/-1.7%,在34oc下50 Ml摇瓶中的生产率为3.88 +/-0.14 Gl-1D-1.对突变体的进一步表征表明,酶的热稳定性和表达增加是l-核糖产量提高的原因.分离出的突变大肠杆菌生产菌株代表了l-核糖大规模生产系统的改进.
Doi:10.2174/138620710791054277

专利信息

专利号:US-2022395800-A1
优先权日:2019-11-04
标题:Device for automated synthesis of oligo- and polysaccharides
发明人：SEEBERGER PETER H; DANGLAD FLORES JOSE; LE MAI HOANG KIM; PARDO VARGAS ALONSO; SLETTEN ERIC; SALWICZEK MARIO
权利人:MAX PLANCK GESELLSCHAT ZU RFERDERUNG DER WSS E V; GlycoUniverse GmbH & Co KG&A
摘要:The present invention generally relates to automated synthesis technology, and more particularly, to a device and method for automated synthesis of oligo- and polysaccharides on a solid support. In particular the present invention relates to a device for automated synthesis of oligo- and polysaccharides on a solid support comprising a reaction vessel, a reagent storing component, a reagent delivery system, a cooling device for cooling reaction vessel, and a pre-cooling device for pre-cooling the reagents to be supplied.

专利号:WO-9839347-A3
优先权日:1997-03-05
标题:Synthesis of l-ribose and 2-deoxy l-ribose
发明人：JUNG MICHAEL E; XU YUE
权利人:UNIV CALIFORNIA; JUNG MICHAEL E; XU YUE
摘要:A method for synthesizing L-ribose (1) and 2-deoxy L-ribose (12) from inexpensive D-ribose (2) is provided. The 5-O-trityl ribose (3) (prepared in 70 % yield from D-ribose) is reduced with borohydride to give the tetrol (4), which is then peracetylated to the tetraacetate (5). Hydrolysis of the trityl ether followed by Swern oxidation affords the aldehyde (7) via the alcohol (6). This aldehyde is a protected form of L-ribose, being L-ribose 2,3,4,5,-tetraacetate. Mild basic hydrolysis of the acetate affords L-ribose itself (1), thus ending an efficient six-step synthesis of (1) from (2) which proceeds in 39 % overall yield. In a second aspect of the invention, L-ribose is converted into the β-selenophenyl ribofuranoside (10) via the tetraester (9) in 71 % isolated yield for the four steps. Treatment of (10) with tributylstannane and AIBN furnishes in 84 % yield the tribenzoyl 2-deoxy-L-ribofuranoside (11) which, on basic hydrolysis, gives 2-deoxy L-ribose (12) in high yield. In a third aspect of the invention, L-arabinose (13) is converted into 2-deoxy L-ribose (12) via the arabinopyranosyl bromide (14), via similar reductive rearrangement with tributylstannane to give the 2-deoxy ribopyranose tribenzoate (16). Hydrolysis yields 2-deoxy L-ribose. In a third aspect of the invention, L-arabinose is converted into 2-deoxy L-ribose by an alternate route.

专利号:EP-3835306-A1
优先权日:2019-12-13
标题:Microwave-assisted method for synthesis of oligo- and polysaccharides on solid phase
权利人:MAX PLANCK GESELLSCHAFT
摘要:The present invention relates to a method for synthesizing oligo- and polysaccharides using microwave radiation, in particular to a method and a device for automated synthesis of oligo- and polysaccharides.

专利号:US-8853132-B2
优先权日:2008-11-27
标题 :Directed synthesis of oligophosphoramidate stereoisomers
发明人：HEINDL DIETER; KESSLER DIRK; ROESLER ANGELIKA; SEIDEL CHRISTOPH; THUER WILMA
权利人:ROCHE DIAGNOSTICS OPERATIONS
摘要:The trivalent phosphorous atom of a compound is reacted with a reagent in such a manner that a stable phosphate mimetic or a specifier is formed. Phosphoramidites with a phosphorous atom containing at least one hydroxyl residue which is provided with a protective group are reacted for this purpose with a free hydroxyl group: In the first synthesis cycle the hydroxyl group is linked to a solid support via a cleavable or non-cleavable linker. In further synthesis cycles the hydroxyl group is created by cleavage of the protective group from the growing oligomer. This results in formation of a phosphorous acid triester which is reacted with azides. By selecting suitable monomers for the synthesis which have a defined stereoconformation compounds of Formula 1 are produced in a stereocontrolled manner.

专利号:US-7125983-B2
优先权日:2000-11-29
标题:L-nucleic acid derivatives and process for the synthesis thereof
发明人：IIZUKA HAJIME; TOGASHI KAZUHIKO; SUZUKI TSUNEJI
权利人:MITSUI CHEMICALS INC
摘要:A novel method has been found to produce 2,2′-anhydro-1-(β-L-arabinofuranosyl)thymine as a novel useful intermediate compound. A novel method has been further found to produce thymidine from 2,2′-anhydro-1-(β-L-arabinofuranosyl)thymine. A novel method has been further found to L-2′-deoxyribose derivatives as a useful synthetic intermediate through L-2,2′-anhydro-5,6-dihydrocyclouridine derivative. According to these methods, synthesis of various L-nucleic acid derivatives, synthesis of which has been difficult till now.

专利号:US-8093397-B2
优先权日:2001-07-03
标 题:Activators for oligonucleotide synthesis
发明人：SINHA NANDA; ZEDALIS WILLIAM EDWARD; MIRANDA GREGORY KEITH
权利人:SINHA NANDA; ZEDALIS WILLIAM EDWARD; MIRANDA GREGORY KEITH; AVECIA BIOTECHNOLOGY INC
摘要:A process for the synthesis of oligonucleotides using phosphoramidite chemistry is provided. The process employs as activator a 1,1-dioxo-1,2-dihydro-1λ6-benzo[d]isothiazol-3-one, preferably in the presence of an organic base. The 1,1-dioxo-1,2-dihydro-1λ6-benzo[d]isothiazol-3-one is represented by the following structural formula: wherein p is 0 or an integer from 1 to 4; X7 is O or S; R for each occurrence is a substituent, preferably each independently, a halo, a substituted or unsubstituted aliphatic group, —NR11R12, —OR13, —OC(O)R13, —C(O)OR13, or cyano; or two adjacent R groups taken together with the carbon atoms to which they are attached form a six membered saturated or unsaturated ring; R11 and R12 are each, independently, —H, a substituted or unsubstituted aliphatic group, a substituted or unsubstituted aryl group, or a substituted or unsubstituted aralkyl group; and R13 is a substituted or unsubstituted aliphatic group, a substituted or unsubstituted aryl group, or a substituted or unsubstituted aralkyl group. Preferred organic bases are pyridine, 3-methylpyridine, or N-methylimidazole.