专利号:US-2013004979-A1 优先权日:2010-06-11 标 题 :Glycosyltransferase reversibility for sugar nucleotide synthesis and microscale scanning 发明人:THORSON JON S; GANTT RICHARD W; PELTIER-PAIN PAULINE MARIE JEANNE 权利人:WISCONSIN ALUMNI RES FOUND; THORSON JON S; GANTT RICHARD W; PELTIER-PAIN PAULINE MARIE JEANNE 摘要:The present invention generally relates to materials and methods for exploiting glycosyltransferase reversibility for nucleotide diphosphate (NDP) sugar synthesis. The present invention provides engineered glycosyltransferase enzymes characterized by improved reaction reversibility and expanded sugar donor specificity as compared to corresponding non-mutated glycosyltransferase enzymes. Such reagents provide advantageous routes to NDP sugars for subsequent use in a variety of biomedical applications, including enzymatic and chemo-enzymatic glycorandomization.
专利号:US-8637287-B2 优先权日:2010-06-11 标题:Glycosyltransferase reversibility for sugar nucleotide synthesis 发明人:THORSON JON S; GANTT RICHARD W 权利人:THORSON JON S; GANTT RICHARD W; WISCONSIN ALUMNI RES FOUND 摘要:The present invention generally relates to materials and methods for exploiting glycosyltransferase reversibility for nucleotide diphosphate (NDP) sugar synthesis. The present invention provides engineered glycosyltransferase enzymes characterized by improved reaction reversibility and expanded sugar donor specificity as compared to corresponding non-mutated glycosyltransferase enzymes. Such reagents provide advantageous routes to NDP sugars for subsequent use in a variety of biomedical applications, including enzymatic and chemoenzymatic glycorandomization.