CAS: 35807-85-3; Sodium 2-((R)-4-((3R,5S,7S,8R,9S,10S,13R,14S,17R)-3,7-Dihydroxy-10,13-Dimethylhexadecahydro-1H-Cyclopenta[a]Phenanthren-17-yl)Pentanamido)Ethane-1-Sulfonate

上下游产品

CAS号107-35-7 牛磺酸 | CAS号7347-25-3 Taurine sodium salt | CAS号516-35-8 牛磺鹅去氧胆酸

Sodium Taurochenodeoxycholate 在 7α-Hydroxysteroid Dehydrogenases Co-Immobilised On Modified Chitosan Microspheres,还原型辅酶ii(Nadph)四钠盐体系中,用 Aq. Buffer作为反应溶剂,反应 5.0H,以70%的收率获得sodium Tauroursodeoxycholate
参考文献:共固定化 7α-和 7β-Hsdh 作为可回收生物催化剂:利用废弃鸡胆高效生产 Tudca†
标题:共固定化 7α-和 7β-Hsdh 作为可回收生物催化剂:利用废弃鸡胆高效生产 Tudca†
摘要:鸡胆长期以来被认为毫无价值,被当作废物丢弃.鸡胆汁的主要成分是牛磺鹅去氧胆酸(Tcdca),它是牛磺熊去氧胆酸(Tudca)的异构体. Tudca已有效用于治疗多种疾病.本文将7α-和7β-羟基类固醇脱氢酶(Hsdh)共固定在改性壳聚糖微球上,并用作可回收的生物催化剂,用于催化鸡胆汁.使用 Tcdca 作为底物时,催化反应在 4 小时内达到平衡,而使用 Tcdca 需要 1 小时.经过四次连续间歇反应后,对于鸡胆汁的催化,Tcdca的转化率低于40%,Tudca收率约为15%.平衡后tudca收率约为62%,反应产物中tudca含量高达33.16%.此外,实验表明,复合底物中的胆红素,Cu 2+和ca 2+均显着抑制酶的活性.该研究不仅拓宽了鸡胆汁的利用,而且为tudca的制备提供了一条清洁的途径.
Doi:10.1039/C8Ra06798H

专利信息

专利号:US-2012190064-A1
优先权日:2004-10-01
标题 :Feeding buffers, systems, and methods for in vitro synthesis of biomolecules
发明人：KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA
权利人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA; LIFE TECHNOLOGIES CORP
摘要:Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of a feeding solution that includes amino acids and an energy source to an ongoing in vitro synthesis system. The invention also includes methods of using a high-yield in vitro synthesis system to produce large quantities of proteins with incorporated labeled amino acids for analysis by methods such as by NMR. The invention further includes vectors for enhanced production of proteins from nucleic acid templates using in vitro synthesis systems.

专利号:US-2009209032-A1
优先权日:2004-10-01
标 题 :Feeding buffers, systems, and methods for in vitro synthesis of biomolecules

专利号:WO-2006039622-A2
优先权日:2004-10-01
标 题 :Feeding buffers, systems, and methods for in vitro synthesis of biomolecules