CAS: 333338-18-4; Sodium 4-Nitrophenyl Phosphate Hexahydrate

专利信息

专利号:US-4703004-A
优先权日:1984-01-24
标 题 :Synthesis of protein with an identification peptide
发明人：HOPP THOMAS P; BEKTESH SUSAN L; CONLON III PAUL J; MARCH CARL J
权利人:IMMUNEX CORP
摘要:A hybrid polypeptide composed of an identification peptide and a desired functional protein are produced by recombinant DNA techniques. A DNA expression vector is constructed that includes segments of DNA coding for the identification peptide and the desired functional protein. The identification peptide consists of a highly antigenic N-terminal portion and a C-terminal linking portion that connects the identification peptide to the N-terminal of the functional protein. The linking portion of the identification peptide is cleavable at a specific amino acid residue adjacent the functional protein by use of a sequence specific proteolytic enzyme or chemical proteolytic agent. The hybrid polypeptide expressed by the host cells transformed by the cloning vector is removed therefrom and purified by affinity chromatography techniques by use of an immobilized ligand specific to the antigenic portion of the identification peptide. The protein is then cleaved from the isolated hybrid polypeptide with an appropriate proteolic enzyme or chemical agent, thereby releasing the mature functional protein in highly purified, highly active state.

专利号:US-4782137-A
优先权日:1984-01-24
标题:Synthesis of protein with an identification peptide, and hybrid polypeptide incorporating same
发明人：HOPP THOMAS P; BEKTESH SUSAN L; CONLON III PAUL J; MARCH CARL J
权利人:IMMUNEX CORP
摘要:A hybrid polypeptide composed of an identification peptide and a desired functional protein are produced by recombinant DNA techniques. A DNA expression vector is constructed that includes segments of DNA coding for the identification peptide and the desired functional protein. The identification peptide consists of a highly antigenic N-terminal portion and a C-terminal linking portion that connects the identification peptide to the N-terminal of the functional protein. The linking portion of the identification peptide is cleavable at a specific amino acid residue adjacent the functional protein by use of a sequence specific proteolytic enzyme or chemical proteolytic agent. The hybrid polypeptide expressed by the host cells transformed by the cloning vector is removed therefrom and purfied by affinity chromatography techniques by use of an immobilized ligand specific to the antigenic portion of the identification peptide. The protein is then cleaved from the isolated hybrid polypeptide with an appropriate proteolic enzyme or chemical agent, thereby releasing the mature functional protein in highly purified, highly active state.

专利号:US-5738855-A
优先权日:1994-10-17
标题:Synthesis of typhoid fever vaccine from a plant or fruit polysaccharide
发明人：SZU SHOUSUN CHEN; BYSTRICKY SLAVOMIR
权利人:US ARMY
摘要:The present invention is a modified plant, fruit or synthetic oligo- or polysaccharide which has been structurally altered so as to render the modified saccharide antigenically similar to the Vi of Salmonella typhi. The modified saccharide may be conjugated to a carrier to form a conjugate that is immunogenic against S. typhi. Antibodies produced in response to the immunogenic conjugate are protective against typhoid fever. Methods are provided for making the modified saccharide and the immunogenic conjugate.

专利号:US-7901687-B2
优先权日:2006-04-28
标 题 :Antibodies for the detection of Bacillus anthracis and vaccine against B. anthracis infections
发明人：WERZ DANIEL B; SEEBERGER PETER H; TAMBORRINI MARCO; PLUSCHKE GERD
权利人:ETH ZUERICH; SCHWEIZERISCHES TROPENINSTITUT
摘要:The present invention relates to conjugates of oligosaccharides of formula 1, wherein R is a linker to a carrier protein and optionally comprises up to three further saccharides, 5 and which are useful for vaccination, methods of synthesis of such conjugates, antibodies against this antigen, hybridoma producing monoclonal antibodies against this antigen, assays using these antibodies for the detection of B. anthracis spores and kits comprising these antibodies, and a vaccine for the prevention of B. anthracis infection comprising the conjugates of oligosaccharides of formula 1. Monoclonal antibodies 10 according to the invention selectively bind to B. anthracis , but not to related bacteria such as B. subtilis, B. cereus and other bacteria of this group such as B. thuringiensis .

专利号:US-6251663-B1
优先权日:1996-04-24
标题 :Recombinant eukaryotic plasmids containing allergen-gene and use thereof for the prevention and/or treatment of allergic diseases
发明人：HSU CHING-HSIANG; CHUA KAW-YAN; TAO MI-HUA; HSIEH KUE-HSIUNG
权利人:JEN WEN CO LTD
摘要:The present invention relates to recombinant eukaryotic plasmids comprising an eukaryotic expression vector and an allergen gene for the prevention and/or treatment of allergic diseases. When the recombinant vector containing allergen-gene administrate to an individual in need of such prevention and/or treatment by intramuscular injection, intranasal delivery or intratracheal delivery, the production of allergen-specific IgE synthesis can be inhibited. The invention also relates to the pharmaceutical compositions comprising the the recombinant vector for use in the the prevention and/or treatment of allergic diseases and the production of allergen-specific IgE synthesis. A method for the prevention and/or treatment of allergic diseases is also provided.

专利号:EP-0658564-B1
优先权日:1986-04-30
标题 :Electroluminescent compounds and intermediates in their synthesis
发明人：MASSEY RICHARD J; POWELL MICHAEL J; MIED PAUL A; POONIAN MOHINDAR S; FENG PETER; CIANA LEOPOLDO DELLA; DRESSICK WALTER J
权利人:IGEN INT INC
摘要:Novel compounds for use in electrochemiluminescent assays and characterised by containing the structure        X - Y - ZwhereinX represents one or more nucleotides which may be the same or different, one or more amino acids which may be the same or different, an antibody, an analyte of interest or an analogue of an analyte of interestY represents a linker group attached to X and Z,andZ represents an electrochemiluminescent moiety, and intermediates employed in their synthesis.