CAS: 9002-68-0; Follicle Stimulating Hormone

专利信息

专利号:US-2024167006-A1
优先权日:2022-06-09
标题 :High production of recombinant protein by making cell hybrids and enriching for a preferred mitochondrial phenotype
发明人：FORM LAWRENCE; NGO KATHY
权利人:CHO PLUS INC
摘要:This disclosure provides a technology for producing cells and cell lines that are capable of manufacturing a recombinant protein more efficiently and in a more cost effective manner. Cells are engineered or fused together to make a population of cell hybrids. The hybrids are then enriched for a mitochondria phenotype that supports and enhances protein synthesis and secretion. The phenotype may include a higher content of mitochondria per cell, a high or low cellular content of reactive oxygen species (ROS), and/or a high or low mitochondria membrane potential. The hybrids may also be enriched for a high content of endoplasmic reticulum. Producer cell lines obtained in this fashion may produce as much as 10 or 15 times the amount of protein per cell than the parental cell line. The producer cells can be used for efficient manufacture of recombinant proteins suitable for use in any industrial process, such as the making of pharmaceutical agents and food ingredients.

专利号:WO-2025128096-A1
优先权日:2023-12-13
标题:High production of recombinant protein by making cell hybrids and enriching for a preferred mitochondrial phenotype
发明人：FORMAN LAWRENCE; NGO KATHY
权利人:CHO PLUS INC
摘要:This disclosure provides a technology for producing cells and cell lines that are capable of manufacturing a recombinant protein more efficiently and in a more cost effective manner. Cells are engineered or fused together to make a population of cell hybrids. The hybrids are then enriched for a mitochondria phenotype that supports and enhances protein synthesis and secretion. The phenotype may include a higher content of mitochondria per cell, a high or low cellular content of reactive oxygen species (ROS), and/or a high or low mitochondria membrane potential. The hybrids may also be enriched for a high content of endoplasmic reticulum. Producer cell lines obtained in this fashion may produce as much as 10 or 15 times the amount of protein per cell than the parental cell line. The producer cells can be used for efficient manufacture of recombinant proteins suitable for use in any industrial process, such as the making of pharmaceutical agents and food ingredients.