CAS: 13114-27-7; Zeatin Mixed Isomers-Plant Cell Culture

上下游产品

2,6 dichloropurine (Z)-4-amino-2-methyl-2-buten-1-ol oxalate (2:1) 6-chloropurine (2R,3S,4S,5R,6R)-2-Hydroxymethyl-6-[(Z)-2-methyl-4-(9H-purin-6-ylamino)-but-2-enyloxy]-tetrahydro-pyran-3,4,5-trioltrans-zeatin riboside (Z)-4-amino-2-methyl-2-buten-1-ol hypoxanthine

4-[(2-Chloro-7H-Purin-6-yl)Amino]-2-Methylbut-2-En-1-Ol置于二氯二茂钛,异丙基溴化镁,水体系中,用 四氢呋喃 用作溶剂,化学反应 24.0H,以46%的收率获得玉米素
参考文献:Peroxide-Shunt Substrate-Specificity For The Salmonella Typhimurium O2-Dependent Trna Modifying Monooxygenase (Miae)
标题:Peroxide-Shunt Substrate-Specificity For The Salmonella Typhimurium O2-Dependent Trna Modifying Monooxygenase (Miae)
摘要:Post-Transcriptional Modifications Of Trna Are Made To Structurally Diversify Trna. These Modifications Alter Noncovalent Interactions Within The Ribosomal Machinery,Resulting In Phenotypic Changes Related To Cell Metabolism,Growth,And Virulence. Miae Is A Carboxylate Bridged,Nonheme Diiron Monooxygenase,Which Catalyzes The O-2-Dependent Hydroxylation Of A Hypermodified-Trna Nucleoside At Position 37 (2-Methylthio-N-6-Isopentenyl-Adenosine(37)-Trna) [designated Ms(2)I(6)A(37)]. In This Work,Recombinant Miae Was Cloned From Salmonella Typhimurium,Purified To Homogeneity,And Characterized By Uv-Visible And Dual-Mode X-Band Epr Spectroscopy For Comparison To Other Nonheme Diiron Enzymes. Additionally,Three Nucleoside Substrate-Surrogates (I(6)A,Cl(2)I(6)A,And Ms(2)I(6)A) And Their Corresponding Hydroxylated Products (Io(6)A,Cl(2)Io(6)A,And Ms(2)Io(6)A) Were Synthesized To Investigate The Chemo-And Stereospecificity Of This Enzyme. In The Absence Of The Native Electron Transport Chain,The Peroxide-Shunt Was Utilized To Monitor The Rate Of Substrate Hydroxylation. Remarkably,Regardless Of The Substrate (I(6)A,Cl(2)I(6)A,And Ms(2)I(6)A) Used In Peroxide-Shunt Assays,Hydroxylation Of The Terminal Isopentenyl-C4-Position Was Observed With >97% E-Stereoselectivity. No Other Nonspecific Hydroxylation Products Were Observed In Enzymatic Assays. Steady-State Kinetic Experiments Also Demonstrate That The Initial Rate Of Miae Hydroxylation Is Highly Influenced By The Substituent At The C2-Position Of The Nucleoside Base (V(0)/[e] For Ms(2)I(6)A > I(6)A > Cl(2)I(6)A). Indeed,The >3-Fold Rate Enhancement Exhibited By Miae For The Hydroxylation Of The Free Ms(2)I(6)A Nucleoside Relative To I(6)A Is Consistent With Previous Whole Cell Assays Reporting The Ms(2)Io(6)A And Io(6)A Product Distribution Within Native Trna-Substrates. This Observation Suggests That The Nucleoside C2-Substituent Is A Key Point Of Interaction Regulating Miae Substrate Specificity:
Doi:10.1021/bi4000832

专利信息

专利号:US-2024110196-A1
优先权日:2022-08-29
标题 :Use of multigene stacking method in synthesis of nervonic acid in brassica napus
发明人：LIU FANG; WANG PANDI; WU GANG; XIONG XIAOJUAN
权利人:OIL CROPS RES INSTITUTE CAAS
摘要:The present disclosure relates to use of a multigene stacking method in synthesis of nervonic acid in Brassica napus. The present disclosure provides a multigene co-expression plant vector, including an initial backbone of pBWA(V)BII and multiple exogenous gene expression cassettes. The present disclosure further provides two plant vectors applicable to genetic transformation, including a three-gene co-expression plant vector and a five-gene co-expression plant vector. In the present disclosure, after the three-gene co-expression plant vector and the five-gene co-expression plant vector are separately transferred into the Brassica napus, the nervonic acid (NA) with a high content is synthesized in the Brassica napus through multigene co-expression. An NA ratio can be significantly increased combined with a higher seed oil content and a greater field seed yield. The plant vector realizes efficient synthesis of NA in oil crops, obtains NA-rich seed oil, and increases an added value of edible oil.

专利号:US-9943544-B2
优先权日:2015-03-18
标 题:Process for bio synthesis of nano arsenic trioxide and its use in treatment of diseases including cancer
发明人：BENDALE YOGESH NARAYAN; BENDALE VINEETA YOGESH
权利人:BENDALE YOGESH NARAYAN; BENDALE VINEETA YOGESH
摘要:The present invention is a process for bio synthesis of nano arsenic trioxide defined by its low toxicity, higher bio availability and nano particle size with the aid of buttermilk, goat urine, dolichos biflorous and other plant materials such as ginger, momordica charantia and musa paradisiaca . The invention is carried out in different steps involving purification of crude form of arsenic trioxide by boiling it with buttermilk, goat urine and extract of dolichos biflorous in subsequent steps, followed by the trituration of the bio purified arsenic trioxide with extracts of ginger and momordica charantia in subsequent steps and heating of the dry product obtained after trituration with musa paradisiaca resulting in the production of novel nano arsenic trioxide. The product is effective in the treatment of various diseases including different types of cancer in animals and humans. The product obtained through the process is less toxic with higher bio availability.

专利号:US-7402418-B2
优先权日:2001-09-20
标 题:Genes participating in the synthesis of fatty acid having trans-11-,cis-13-conjugated double bond and utilization thereof
发明人：OSUMI MARI; MURASE JUNKO; IMAMURA JUN
权利人:PLANTECH RES INST; INC ADMIN AGENCY NARO
摘要:An object of the present invention is to clone a gene which is involved in synthesis of fatty acid having trans-11-, cis-13-conjugated double bonds from fatty acid having a double bone at position Δ12. The present invention provides a gene having any one of the following nucleotide sequences:n (A) a nucleotide sequence encoding an amino acid sequence shown in SEQ ID NO: 1 or 12; (B) a nucleotide sequence encoding an amino acid sequence comprising a deletion, addition or substitution of one or several amino acids with respect to the amino acid sequence shown in SEQ NO: 1 or 12, and having an ability of synthesizing fatty acid having trans-11-, cis-13-conjugated double bonds from fatty acid having a double bond at position Δ12; (C) a nucleotide sequence shown in SEQ ID NO: 2 or 13; (D) a nucleotide sequence comprising a deletion, addition or substitution of one or several nucleotides with respect to the nucleotide sequence shown in SEQ ID NO: 2 or 13, and encoding a protein having an ability of synthesizing fatty acid having trans-11-, cis-13-conjugated double bonds from fatty acid having a double bond at position Δ12; and (E) a nucleotide sequence hybridizing with the nucleotide sequence shown in SEQ ID NO: 2 or 13 or a complementary sequence thereof under stringent conditions, and encoding a protein having an ability of synthesizing fatty acid having trans-11-, cis-13-conjugated double bonds from fatty acid having a double bond at position Δ12.

专利号:US-2005223441-A1
优先权日:2002-02-22
标 题:Methods for increasing the amount of protein in potato tubers
发明人：RYAN CLARENCE A; NARVAEZ JULIAN; PEARCE GREGORY L; MCGURL BARRY F
权利人:RYAN CLARENCE A; NARVAEZ JULIAN; PEARCE GREGORY L; MCGURL BARRY F
摘要:In one aspect, the present invention provides potato tubers that each include water-soluble protein at a concentration of at least 5 mg/ml. Some potato tubers of the invention include an exogenous nucleic acid molecule that encodes a systemin molecule, or a prosystemin molecule, that is expressed in the potato tubers, whereby the synthesis of at least one water-soluble protein is induced by the expressed systemin molecule (or the systemin molecule derived from processed prosystemin). In another aspect, the present invention provides methods for increasing the amount of water-soluble protein in a potato tuber.

专利号:WO-2025056767-A1
优先权日:2023-09-15
标题 :Process for the preparation of enantiomerically enriched aliphatic amines
发明人：BOU HAMDAN FARHAN; GODINEAU EDOUARD; SMEJKAL TOMAS; DUMEUNIER RAPHAEL; BOUSSEMGHOUNE MOHAMED ABDELOUAHAB; BLUM MATHIAS
权利人:SYNGENTA CROP PROTECTION AG
摘要:The present invention relates to a process for the preparation of enantiomerically enriched cyclobutylamines of formula (I) from α-tertiary cyclobutanones and reacting said enantiomerically enriched cyclobutylamines to enantiomerically enriched cyclobutylamides. Such compounds are useful intermediates in the synthesis of microbiocidal thiazole compounds.

专利号:US-7598084-B2
优先权日:2003-12-30
标题 :Modifications of plant traits using cyclin A
发明人：FORSTER RICHARD L; CONNETT MARIE B; EMERSON SARAH JANE; GRIGOR MURRAY ROBERT; HIGGINS COLLEEN M; LUND STEVEN TROY; MAGUSIN ANDREAS; KODRZYCKI BOB
权利人:ARBORGEN LLC
摘要:Novel plant polysaccharide synthesis genes and polypeptides encoded by such genes are provided. These genes and polynucleotide sequences are useful regulating polysaccharide synthesis and plant phenotype. Moreover, these genes are useful for expression profiling of plant polysaccharide synthesis genes. The invention specifically provides cell cycle polynucleotide and polypeptide sequences isolated from Eucalyptus and Pinus . The invention also provides for the expression of cyclin A genes to modify the physical traits of a transgenic plant.