CAS: 2140-61-6; 4-Amino-1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(Hydroxymethyl)Tetrahydrofuran-2-yl)-5-Methylpyrimidin-2(1H)-One

相似化合物

113886-70-7 148608-53-1 19235-17-7

上下游产品

CAS号7323-83-3 4-methoxy-5-met... | CAS号59237-68-2 2',3',5'-tri-O-... | CAS号28585-48-0 O2',O3',O5'-tri... | CAS号6974-32-9 1-乙酰氧基-2,3,5-三苯... | CAS号163496-05-7 (2R,3R,4R,5R)-2... | CAS号7288-28-0 O,O'-双(三甲基甲硅烷基)胸腺嘧啶 | CAS号554-01-8 5-甲基胞嘧啶 | CAS号32865-88-6 5-Methyl-N-(tri... | CAS号5151-34-8 2,4-二甲氧基-5-甲基嘧啶 | CAS号554-01-8 5-甲基胞嘧啶 | CAS号1123-95-1 5-羟甲基胞嘧啶 | CAS号19235-17-7 5-羟甲基胞苷 | CAS号160107-15-3 N4-Benzoyl-5-me... | CAS号1463-10-1 5-甲基尿甙

5-甲基胞嘧啶置于sodium Methylate,Tin(Ll) Chloride体系中,用 甲醇,二氯甲烷 用作溶剂,化学反应 24.0H,反应生成5-甲基胞苷
参考文献:Recognition Of Rna By Triplex Formation: Divergent Effects Of Pyrimidine C-5 Methylation
标题:Recognition Of Rna By Triplex Formation: Divergent Effects Of Pyrimidine C-5 Methylation
摘要:In Dna Triple Helices,Methylation At C-5 Of Thymine Or Cytosine Is Reported To Have Similar Stabilizing Effects For Both Bases. Here We Show,However,That Methylation Of The Same Positions In Rna Triplexes Has Distinctly Different Effects Than In Dna. We Have Previously Described The Use Of Circular Tripler-Forming Rna Oligonucleotides To Recognize Rna Sequences. Here It Is Shown That Addition Of C-5 Methyl Groups To Uracils In These Compounds Very Significantly Increases Not Only Affinity But Also Sequence Selectivity In Binding A Purine-Rich Rna Target,As Measured By Thermal Denaturation With Various Target Rnas. Surprisingly,However,Addition Of C-5 Methyl Groups To Cytosines Actually Decreases Affinity In Binding Rna,While The Same Substitution In Dna Is Thermally Stabilizing. Possible Sources Of This Divergent Behavior Are Discussed. A Synthesis Of 5-Methylcytidine Ribonucleoside 2'-O-Silyl-3'-O-Phosphoramidite Is Also Described. (C) 1997 Elsevier Science Ltd.
Doi:10.1016/s0968-0896(97)00059-X

专利信息

专利号:US-6683189-B1
优先权日:1996-02-26
标 题 :Method for the synthesis of pyrrole and imidazole carboxamides on a solid support
发明人：DERVAN PETER B; BAIRD ELDON
权利人:CALIFORNIA INST OF TECHN
摘要:The present invention describes a novel method for the solid phase synthesis of polyamides containing imidazole and pyrrole carboxamides. The polyamides are prepared on a solid support from aromatic carboxylic acids and aromatic amines with high stepwise coupling yields (>99%), providing milligram quantities of highly pure polyamides. The present invention also describes the synthesis of analogs of the natural products Netropsin and Distamycin A, two antiviral antibiotics. The present invention also describes a novel method for the solid phase synthesis of imidazole and pyrrole carboxamide polyamide-oligonucleotide conjugates. This methodology will greatly increase both the complexity and quantity of minor-groove binding polyamides and minor-groove binding polyamide-oligonucleotide conjugates which can be synthesized and tested.

专利号:US-8138330-B2
优先权日:2006-09-11
标 题 :Process for the synthesis of oligonucleotides
发明人：LEUCK MICHAEL; WOLTER ANDREAS; STUMPE ALFRED
权利人:LEUCK MICHAEL; WOLTER ANDREAS; STUMPE ALFRED; SIGMA ALDRICH CO LLC
摘要:The present invention discloses novel methods for the synthesis of oligonucleotides with nucleoside phosphoramidites on solid supports. The methods comprise the stepwise chain assembly of oligonucleotides on supports with 5′-acyl phosphoramidites. The synthesis cycles consist of a front end deprotection step which is conducted with a solution of a primary amine or a phenolate, a phosphoramidite coupling step with a 5′-acyl nucleoside phosphoramidite in the presence of an activator, a phosphite oxidation step and an optional capping step. The novel methods improve the quality of synthetic oligonucleotides due to the irreversibility of the front end deprotection step, which prevents the formation of deletion sequences, and due to the avoidance of acidic reagents in the synthesis cycles, which prevent the formation of depurination side products. The invention further discloses novel nucleoside phosphoramidite compositions wherein the phosphoramidites carry acyl front end protective groups which are cleavable with primary amines or phenolates. The invention is applicable to the synthesis of oligodeoxyribonucleotides, oligoribonucleotides and oligonucleotides with modifications in their sugar or phosphate groups.

专利号:US-2005130201-A1
优先权日:2003-10-14
标 题 :Splint-assisted enzymatic synthesis of polyribounucleotides
发明人：DERAS MICHAEL; PLEISS JEFFREY A; SCARINGE STEPHEN
权利人:DHARMACON INC
摘要:The present invention comprises methods and compositions for splint-assisted enzymatic synthesis of polyribonucleotides using an RNA polymerizing enzyme. The invention provides ligating ribonucleotides comprising ligating a donor RNA molecule to an acceptor RNA molecule in the presence of RNA ligase and a splint, wherein the donor RNA molecule is comprised of at least one nucleotide and a ligation linker moiety, the acceptor RNA molecule is comprised of at least one nucleotide and a ligation linker moiety and the splint is comprised of a polyribonucleotide. The invention also provides splints for use in splint-assisted enzymatic synthesis using an RNA polymerizing enzyme.

专利号:US-2003049619-A1
优先权日:2001-03-21
标题 :Methods for the synthesis of polynucleotides and combinatorial libraries of polynucleotides
发明人：DELAGRAVE SIMON; MARRS BARRY
摘要:Methods for the synthesis of polynucleotides and derivatives thereof are provided. Methods for the preparation of combinatorial libraries of polynucleotides are also provided. In addition, methods for the preparation and identification of polynucleotides having a predetermined property are provided.

专利号:US-11407993-B2
优先权日:2017-02-14
标 题 :Method for the synthesis of DNA conjugates by micellar catalysis
发明人：BHAT AVINASH SHASHIDHAR; KLIKA SKOPIC MATEJA; BRUNSCHWEIGER ANDREAS; WEBERSKIRCH RALF
权利人:UNIV DORTMUND TECH
摘要:A method for the synthesis of a chimeric conjugate molecule by micellar catalysis that may form part of DNA-encoded compound libraries. A DNA-coupled organic starter molecule may be reacted with another organic compound, using a catalyst located within a micelle, to form a conjugate of an organic candidate compound coupled to a DNA identifier tag.

专利号:US-7777023-B2
优先权日:1998-10-23
标题 :Method for the chemical synthesis of oligonucleotides
发明人：VARGEESE CHANDRA; SHAFFER CHRISTOPHER; WANG WEIMIN
权利人:SIRNA THERAPEUTICS INC
摘要:The present invention features novel compositions, linkers, derivatized solid supports, and methods for the efficient solid phase synthesis of oligonucleotides, including RNA, DNA, RNA-DNA chimeras, and analogs thereof.