专利号:US-6350595-B1 优先权日:1997-10-28 标题:Synthesis of polynucleotides having random sequences 发明人:NEUNER PHILIPPE 权利人:ISTITUTO DI RICERCHE DI MOLECO 摘要:Subject of this invention is a process for the chemical synthesis of polynucleotides having totally or partially random sequences, based on the utilization, as synthesis monomer units for the random sequence part, or presynthesized mononucleotides and dinucleotides. Said synthesis is carried out on separate supports, so that on each of those supports is alternated at least one reaction cycle wherein a mixture of said dinucleotides is bound, with at least one reaction cycle wherein a mononucleotide is bound, and that in a preferred embodiment at the end of the n cycles required for a codon synthesis, the supports are mixed and then redivided into one or more reaction containers. The resulting polynucleotides are such that, for the random sequence part, each trinucleotide unit is fit to match only a limited number of codons, predefined for each unity in number and sequence, and the genetic code degeneracy effects can thus be eliminated. FIG. 1 shows the chemical structure of the dinucleotides utilized as monomer units for the synthesis of codons forming the final sequence.
专利号:WO-9312076-A1 优先权日:1991-12-13 标题:Reagents for automated synthesis of peptide analogs 发明人:WEBB THOMAS ROY 权利人:CORVAS INT INC 摘要:Reagents suitable for synthesis of peptide analogs using automated peptide synthesis and procedures for synthesis of peptide analogs are provided.
专利号:US-10865390-B2 优先权日:2018-02-12 标 题 :Alcohol dehydrogenase mutant and application thereof in synthesis of diaryl chiral alcohols 发明人:NI YE; ZHOU JIEYU; XU GUOCHAO; WANG YUE 权利人:UNIV JIANGNAN 摘要:The present disclosure discloses an alcohol dehydrogenase mutant and application thereof in synthesis of diaryl chiral alcohols, and belongs to the technical field of bioengineering. The alcohol dehydrogenase mutant of the present disclosure has excellent catalytic activity and stereoselectivity, and may efficiently catalyze the preparation of a series of chiral diaryl alcohols in R- and S-configurations. By coupling alcohol dehydrogenase of the present disclosure to glucose dehydrogenase or formate dehydrogenase, the synthesis of chiral diaryl alcohol intermediates of various antihistamines may be achieved. Compared with the prior art, a method for preparing diaryl chiral alcohols through asymmetric catalytic reduction using the alcohol dehydrogenase of the present disclosure has the advantages of simple and convenient operation, high substrate concentration, complete reaction and high product purity, and has great industrial application prospects.
专利号:US-5283293-A 优先权日:1990-12-14 标 题:Reagents for automated synthesis of peptide analogs 发明人:WEBB THOMAS R 权利人:CORVAS INC 摘要:Reagents suitable for synthesis of peptide analogs using automated peptide synthesis and procedures for synthesis of peptide analogs are provided.
专利号:WO-2008096846-A1 优先权日:2007-02-09 标 题 :Composition for synthesis of protein labeled with stable isotope and method for production of protein labeled with stable isotope 发明人:YOKOYAMA SHIGEYUKI; KIGAWA TAKANORI; YOKOYAMA JUN 权利人:RIKEN; TAIYO NIPPON SANSO CORP; YOKOYAMA SHIGEYUKI; KIGAWA TAKANORI; YOKOYAMA JUN 摘要:Disclosed is a means for synthesizing a protein labeled with a stable isotope (which is useful in the fields of nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry) at low cost. Specifically disclosed is a composition for use in the synthesis of a protein labeled with a stable isotope, which comprises (A) an amino acid mixture comprising 16 kinds of amino acids consisting of L-alanine, L-arginine, L-aspartic acid, L-glutamic acid, glycine, L-histidine, L-isoleucine, L-leucine, L-lysine, L-methionine, L-phenylalanine, L-proline, L-serine, L-threonine, L-tyrosine and L-valine, (B) an indole, (C) hydrogen sulfide or sodium sulfide and (D) an ammonium salt, wherein at least one of the amino acids in the amino acid mixture and/or any one or all of the indole, hydrogen sulfide or the sulfide salt and the ammonium salt is labeled with a stable isotope. Also specifically disclosed is a method for the synthesis of a protein labeled with a stable isotope using the composition.
专利号:US-10822593-B2 优先权日:2018-02-12 标 题:Alcohol dehydrogenase mutant and application thereof in synthesis of diaryl chiral alcohols 发明人:NI YE; ZHOU JIEYU; XU GUOCHAO; WANG YUE 权利人:UNIV JIANGNAN 摘要:The present disclosure discloses an alcohol dehydrogenase mutant and application thereof in synthesis of diaryl chiral alcohols, and belongs to the technical field of bioengineering. The alcohol dehydrogenase mutant of the present disclosure has excellent catalytic activity and stereoselectivity, and may efficiently catalyze the preparation of a series of chiral diaryl alcohols in R- and S-configurations. By coupling alcohol dehydrogenase of the present disclosure to glucose dehydrogenase or formate dehydrogenase, the synthesis of chiral diaryl alcohol intermediates of various antihistamines may be achieved. Compared with the prior art, a method for preparing diaryl chiral alcohols through asymmetric catalytic reduction using the alcohol dehydrogenase of the present disclosure has the advantages of simple and convenient operation, high substrate concentration, complete reaction and high product purity, and has great industrial application prospects.
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合成参考文献
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