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鸟苷置于purine Nucleoside Phosphorylase From Aeromonas Hydrophila Ii体系中,用 Aq. Phosphate Buffer 作为反应溶剂,化学反应生成 肌苷
参考文献:开发固定的核苷磷酸化酶集合以制备核苷类似物:阿拉伯糖基腺嘌呤和2',3'-脱氧肌苷的酶促合成
标题:开发固定的核苷磷酸化酶集合以制备核苷类似物:阿拉伯糖基腺嘌呤和2',3'-脱氧肌苷的酶促合成
摘要:核苷磷酸化酶(nps; Ec 2.4.2.N)的使用是合成天然和修饰核苷的化学方法的便捷替代方法.我们从细菌病原体科氏柠檬酸杆菌,产气荚膜梭菌和化脓链球菌(ck Pnpi,Ck Pnpii,Cp Up,Spup)及其底物特异性针对天然嘧啶或嘌呤核苷及一些类似物,即阿拉伯糖基腺嘌呤(araa)和2',3'-二脱氧肌苷(ddi)进行了研究.观测到对后者化合物的活性为2-3%(与天然底物相比).将酶活性与枯草芽孢杆菌(bs Pynp)的嘧啶核苷磷酸化酶和嗜水气单胞菌(ah)的嘌呤核苷磷酸化酶的特异性进行了比较(ahpnpii)以前由一些作者报道过.对araa和ddi合成具有合适特异性的酶被固定在醛-琼脂糖上.固定的制剂在碱性ph下和在甲醇或乙腈作为助溶剂存在下是高度稳定的.它们通过单罐双酶转糖基化反应分别用于74%和44%的转化,用于araa和ddi的合成.
DOI:10.1002/cplu.201200278

专利信息

专利号:WO-2024153642-A1
优先权日:2023-01-16
标题 :Scarless template-free enzymatic synthesis of polynucleotides
发明人：HORGAN ADRIAN; VERARDO DAMIANO; ADELIZZI BEATRICE; RODRIGUEZ-PINZON DANIEL; THOMEE EMMA
权利人:DNA SCRIPT
摘要:Methods are provided for template-free enzymatic synthesis of polynucleotides on a solid support using double cleavage to produce scarless polynucleotides. In particular, methods are disclosed that use an initiator that comprises a cleavable group, which can be cleaved by a small molecule chemical agent, adjusting pH, heat, or photocleaved by illumination with light to release polynucleotides from a solid support after enzymatic synthesis is completed, and an enzymatic cleavage site, which can be cleaved by a cleavage enzyme to remove polynucleotide scars.

专利号:US-2021222219-A1
优先权日:2015-07-07
标题:Enzymatic synthesis of nucleic acid sequences
发明人：KRANZ CHRISTIAN; LISS MICHAEL; TREFZER AXEL
权利人:THERMO FISHER SCIENT GENEART GMBH
摘要:The disclosure generally relates to compositions and methods for the enzymatic synthesis of nucleic acid sequences. In particular embodiments 3′ protected nucleotides are used in conjunction with a universal template to direct synthesis.

专利号:US-2012178710-A1
优先权日:2009-07-01
标题 :Synthesis of cyclic diguanosine monophosphate and thiophosphate analogs thereof
发明人：JONES ROGER A; GAFFNEY BARBARA L
权利人:JONES ROGER A; GAFFNEY BARBARA L; UNIV RUTGERS
摘要:The invention provides methods for the synthesis of cyclic dinucleotides and thiophosphate analogs thereof as well as a new family of analogs of cyclic diguanosine monophosphate that includes a series of seven phosphorothioate derivatives that includes diastereomers of mono-, di-, and trithiophosphates.

专利号:US-8350022-B2
优先权日:2008-04-04
标题 :Method for the stereoselective synthesis of phosphorus compounds
发明人：MEIER CHRIS; THOMANN JENS O
权利人:UNIV HAMBURG; MEIER CHRIS; THOMANN JENS O
摘要:The present invention relates to a method for stereoselective synthesis of phosphorus compounds, whereby in the first reaction step a chiral auxiliary on the phosphorus atom of phosphoryl chloride, thiophosphoryl chloride or phosphorus trichloride is covalently bonded, the product from the first reaction step is reacted in the following step with an alcohol, thiol, or amine as the nucleophile in the presence of a base, and in the last step the chiral auxiliary is displaced from the product of the following step by a nucleophile.

专利号:US-5629202-A
优先权日:1994-07-19
标题:Computer-controlled bioreactor system for enzymatic synthesis of L-tryptophan
发明人：SU CHEIN-SHYONG; CHAN ZEN-JEN; HUANG WUEN-HSIAN; TSAI HSIN
权利人:DEV CT BIOTECHNOLOGY
摘要:Disclosed is an automatic computer-controlled bioreactor system for enzymatic synthesis of L-tryptophan which comprises a bioreactor linked to a computer equipped with an on-line indole assay device, in which the computer controls the feeding of indole, pyruvic acid and/or its salt and an ammonium salt into the bioreactor and feedback-controls the reaction in the bioreactor by indole concentration determined by the on-line indole assay device, wherein the feeding of indole and ammonium salt is controlled by a pre-defined profile that agrees with a tryptophanase activity profile and pyruvic acid and/or its salt is added at a predetermined time.

专利号:US-4849513-A
优先权日:1983-12-20
标 题 :Deoxyribonucleoside phosphoramidites in which an aliphatic amino group is attached to the sugar ring and their use for the preparation of oligonucleotides containing aliphatic amino groups
发明人：SMITH LLOYD M; FUNG STEVEN; KAISER ROBERT J
权利人:CALIFORNIA INST OF TECHN
摘要:The invention consists of compounds and methods for the synthesis of oligonucleotides which contain one or more free aliphatic amino groups attached to the sugar moieties of the nucleoside subunits. The synthetic method is versatile and general, permitting amino groups to be selectively placed at any position on oligonucleotides of any composition or length which is attainable by current DNA synthetic methods. Fluorescent dyes or other detectable moieties may be covalently attached to the amino groups to yield the corresponding modified oligonucleotide.