CAS: 600-18-0; 2-Oxobutanoic Acid

相似化合物

3952-66-7 5077-67-8 15933-07-0

上下游产品

CAS号20016-85-7 (R)-2-羟基丁酸 | CAS号590-93-2 2-丁炔酸 | CAS号328-50-7 α-酮戊二酸 | CAS号2623-91-8 D-氨基丁酸 | CAS号22059-21-8 1-氨基环丙烷羧酸 | CAS号88126-31-2 (Z)-2-methoxy-2... | CAS号600-15-7 DL-2-羟基丁酸 | CAS号642-93-3 2-oxalopropioni... | CAS号3952-66-7 2-氧代丁酸甲酯 | CAS号3347-90-8 S-2-羟基丁酸 | CAS号1492-24-6 L-2-氨基丁酸 | CAS号93-55-0 苯丙酮 | CAS号515-30-0 苯乳酸水合物 | CAS号938-46-5 2',5'-二羟基苯乙酮 | CAS号2623-91-8 D-氨基丁酸 | CAS号127-17-3 丙酮酸 | CAS号554-12-1 丙酸甲酯 | CAS号2835-81-6 DL-2-氨基丁酸

Dl-高半胱氨酸置于potassium Phosphate,磷酸吡哆醛,Methionine γ-Lyase From Citrobacter Freundii Variant V358Y,还原型辅酶ⅰ,1,4-二巯基-2,3-丁二醇体系中,化学反应生成 2-酮丁酸
参考文献:弗氏柠檬酸工程蛋氨酸γ-裂合酶的抗癌活性
标题:弗氏柠檬酸工程蛋氨酸γ-裂合酶的抗癌活性
摘要:蛋氨酸生物合成不足的蛋氨酸对癌细胞的剥夺已被设想为降低癌细胞生存力的治疗策略.蛋氨酸γ-裂合酶(mgl)是一种降解蛋氨酸的酶,已被用于从癌细胞环境中选择性去除氨基酸.为了增加mgl的催化活性,我们对来自各种微生物的mgl进行了序列和结构保守性分析.尽管发现活性位点和二聚体界面处的大多数残基是保守的,但发现形成活性位点进入通道壁的c末端柔性环中的残基是可变的.因此,我们在c末端柔性环的四个独立位置,即mgl的p357,V358,P360和a366处进行了位点饱和诱变弗氏柠檬酸杆菌,产生被筛选活性的文库.在活性变体中,V358Y的催化速率提高了1.9倍,K M升高了3倍,从而产生了类似于野生型mgl的催化效率.对一组癌症和非恶性细胞系评估了v358Y的细胞毒活性,发现其ic 50低于野生型.v358Y Mgl的3D结构与其他mgl可用结构的比较表明,C末端环处于不依赖于位置358氨基酸的开放或
DOI:10.1016/j.Bbapap.2018.09.011

专利信息

专利号:US-8153839-B2
优先权日:2005-09-14
标题:Method for synthesis of keto acids or amino acids by hydration of acetylene compound
发明人：OGO SEIJI; FUKUZUMI SHUN-ICHI
权利人:OGO SEIJI; FUKUZUMI SHUN-ICHI; JAPAN SCIENCE & TECH AGENCY
摘要:An object of the present invention is to provide a method for synthesis of keto acids by hydration of an acetylene compound (acetylene-carboxylic acids) under mild conditions free from harmful mercury catalysts and a method for synthesis of amino acids from acetylene-carboxylic acids in a single container (one-pot or tandem synthesis). In one embodiment of the method according to the present invention for synthesis of keto acids, acetylene-carboxylic acids is hydrated in the presence of a metal salt represented by General Formula (1), n nwhere M 1 represents an element in Group VIII, IX, or X of the periodic table, and X 1 , X 2 , or X 3 ligand represents halogen, H 2 O, or a solvent molecule, and k represents a valence of a cation species, and Y represents an anion species, and L represents a valence of the anion species, and each of K and L independently represents 1 or 2, and k×m=L×n.

专利号:US-10920229-B2
优先权日:2014-07-16
标 题:Method for improving heterologous synthesis of Escherichia coli into polyketides and use of same
发明人：WANG YONG; XIONG ZHIQIANG; SONG SHUJIE; LIU QIAOXIA; WANG JIANFENG
权利人:SHANGHAI INST BIOLOGICAL SCIENCES CAS; CAS CENTER FOR EXCELLENCE IN MOLECULAR PLANT SCIENCES
摘要:The present invention relates to a method for improving the heterologous synthesis of a polyketide by E. coli and use thereof. The yield of the polyketide heterologously synthesized by E. coli is significantly increased by attenuating the expression of seventy-two genes, such as sucC and talB, in a host strain, wherein the highest yield increase rate can reach 60% or more. Currently, erythromycin is the most clear model compound in the study on the biosynthesis of polyketids. The production strain of the present invention enables massive accumulation of 6-deoxyerythronolide (6-dEB), an erythromycin precursor, in the fermentation process, laying the foundation for the industrial production of the heterologous synthesis of erythromycin by E. coli.

专利号:US-5861532-A
优先权日:1997-03-04
标 题:Solid-phase synthesis of N-alkyl amides
发明人：BROWN EDWARD G; NUSS JOHN M
权利人:CHIRON CORP
摘要:Methods for solid phase synthesis of N-alkyl amides comprise reductive amination of carbonyl compounds using a reducing agent and an amine-containing linker bound to a solid support. The methods afford high yields of linker-bound, monoalkylated amines, and subsequent coupling with acid derivatives provide derivatized N-substituted amides in excellent yields after cleavage from the solid-phase. Compositions useful in solid phase synthesis are also described.

专利号:WO-2024012791-A1
优先权日:2022-07-11
标题 :Electrochemical synthesis of pyrazolines and pyrazoles
发明人：BÄR ROBIN MAXIMILIAN; FORD MARK JAMES; KALDAS SHERIF JAMES; WALDVOGEL SIEGFRIED; HOFMANN SILJA; LINDEN MARTIN
权利人:BAYER AG
摘要:The present invention relates to an electrochemical process for the synthesis of pyrazolines and pyrazoles of formula (I). The process can be especially used for the synthesis of the herbicide safener mefenpyr-diethyl.

专利号:US-11046978-B2
优先权日:2016-03-16
标 题:Synthesis of isoprenoids and derivatives
发明人：GONZALEZ RAMON; CLOMBURG JAMES M; CHEONG SEOKJUNG
权利人:UNIV RICE WILLIAM M
摘要:This disclosure generally relates to the use of enzyme combinations or recombinant microbes comprising same to make isoprenoid precursors, isoprenoids and derivatives thereof including prenylated aromatic compounds. Novel metabolic pathways exploiting Claisen, aldol, and acyioin condensations are used instead of the natural mevalonate (MVA) pathway or 1-deoxy-d-xylulose 5-phosphate (DXP) pathways for generating isoprenoid precursors such as isopentenyl pyrophosphate (IPP), dimethylallyl pyrophosphate (DMAPP), and geranyl pyrophosphate (GPP). These pathways have the potential for better carbon and or energy efficiency than native pathways. Both decarboxylative and non-carboxylative condensations are utilized, enabling product synthesis from a number of different starting compounds. These condensation reactions serve as a platform for the synthesis of isoprenoid precursors when utilized in combination with a variety of metabolic pathways and enzymes for carbon rearrangement and the addition/removal of functional groups. Isoprenoid alcohols are key intermediary products for the production of isoprenoid precursors in these novel synthetic metabolic pathways.

专利号:US-4334969-A
优先权日:1980-07-04
标题 :Photochemical process for the synthesis of strobilurin
发明人：STEGLICH WOLFGANG; SCHRAMM GEORG; ANKE TIMM; OBERWINKLER FRANZ
权利人:HOECHST AG
摘要:Process for stereoselective synthesis of strobilurin in the (E) configuration, starting from cinnamaldehyde and 2-oxobutyric acid.