专利号:US-12421534-B2 优先权日:2021-11-10 标 题 :Engineered enzymes and method for the synthesis of diverse tyrosine analogs 发明人:ALMHJELL PATRICK J; ARNOLD FRANCES H 权利人:CALIFORNIA INST OF TECHN 摘要:Provided herein is an engineered tryptophan synthase β-subunit (TrpB) that catalyzes the synthesis of tyrosine, tyrosine analogs, or salts thereof. Also provided herein are methods for preparing tyrosine, tyrosine analogs, or a salt thereof using the engineered TrpB described herein.
专利号:WO-2024235339-A1 优先权日:2023-05-29 标 题:Blue grain gene of thinopyrum bessarabicum for regulating synthesis of anthocyanins and use thereof 发明人:FU DAOLIN; QI ZENGJUN; WANG WENQIANG; WANG QING; LI XIAOHAN; LIU WENDI; MA XUHUI; Hao Qunqun; HE ZIMING 权利人:SPRING VALLEY AGRISCIENCE CO LTD; UNIV NANJING AGRICULTURAL 摘要:A blue grain gene of Thinopyrum bessarabicum for regulating synthesis of anthocyanins and the use thereof, belonging to the technical field of plant genetic engineering. The blue grain gene of Thinopyrum bessarabicum is the TbMYB4J gene or TbMYC4J gene, the nucleotide sequence of the TbMYB4J gene being as shown in SEQ ID NO. 1, and the nucleotide sequence of the TbMYC4J gene being as shown in SEQ ID NO. 2 or SEQ ID NO. 3. For the first time, the two blue grain genes TbMYB4J and TbMYC4J for regulating the synthesis of anthocyanins are identified and obtained from the 4J chromosome of Thinopyrum bessarabicum, and influences of different combinations of the TbMYB4J and TbMYC4J genes on the accumulation of anthocyanins are defined by means of transient genetic transformation technology, thereby providing a basis and novel resources for color markers in molecular marker-assisted selection breeding and cross-breeding systems of wheat.
专利号:WO-2023019298-A1 优先权日:2021-08-20 标 题:Improved method of synthesis of 1-(3',4'-methylene dioxyphenyl)-2-(methylamino) propane (mdma) 发明人:O'MALLEY WILLIAM IAN; WYNNE PAUL MICHAEL 权利人:MIND MEDICINE AUSTRALIA LTD 摘要:The present invention relates to an improved method of synthesis of 1-(3',4'-methylenedioxyphenyl)-2-(methylamino)propane (MDMA) that is of sufficient purity that it can be used for pharmaceutical applications. In particular the present invention provides a method of synthesising MDMA comprising the steps of (a) condensation of piperonal and nitroethane in the presence of a catalysing base to form 1-(3',4'-methylenedioxyphenyl)-2-nitroprop-1-ene (MDP-2-nitropropene), (b) oxidative hydrolysis of MDP-2-nitropropene to form 1-(3',4'methylenedioxyphenyl)-propan-2-one (MDP2P), and (c) reductive amination of MDP2P to 1-(3',4'-methylenedioxyphenyl)-2-(methylamino)propane (MDMA).
专利号:US-2024383940-A1 优先权日:2021-03-31 标题 :Synthesis of trinucleotide and tetranucleotide caps for mrna production 发明人:ENDO ATSUSHI 权利人:MODERNA TX INC 摘要:Provided herein are methods of making trinucleotides and tetranucleotides for use as 5′ mRNA caps. The methods utilize a novel “top-downâ€? strategy and provide for synthesis of oligonucleotides with higher yields and increased efficiency compared to traditional methods. A key step of the methods disclosed, herein can also be adapted to utilize a “one-potâ€? approach, resulting in an increase in the yield of the final oligonucleotide product.
专利号:WO-2025181736-A1 优先权日:2024-03-01 标 题 :Continuous-flow synthesis of amine-sulfonic acid buffers 发明人:NAYAK NAGARAJ; Manohar Venkatachalam; SIVAKUMAR SREERAMAGIRI; Venkata Ramanarayanan GANAPATHY BHOTLA; SHIVANI MUKESH KUMAR; M TAMIZHAZHAGAN; SUNDAR SHYAM; ABDUR RAHMAN JUNAID NAYEEM SHAIK 权利人:SRAVATHI ADAVANCE PROCESS TECH PRIVATE LIMITED 摘要:The instant invention discloses a continuous flow synthesis of amine-sulfonic acid buffers, specifically biological buffers such as MOPS, MOBS, CAPS, CABS by the reaction of amines such as morpholine, N-cyclohexylamine with corresponding sulfo-alkylating agents or alkane- sultones, in a solvent-free method wherein, an agitated thin film dryer (AFTD) is used to remove the excess of the amine-reagents such as morpholine, cyclohexylamine, so as to obtain the final products namely the buffers in a dried condition. The invention also discloses an economically viable, environmentally friendly process for obtaining biological buffers such as MOPS, MOBS, CAPS, CABS wherein the unreacted reagent/solvent is recycled to the continuous- flow-reactor.
专利号:US-12037623-B2 优先权日:2018-07-09 标 题 :Enzymatic synthesis of 4′-ethynyl nucleoside analogs 发明人:HUFFMAN MARK A; FRYSZKOWSKA ANNA; KOLEV JOSHUA N; DEVINE PAUL N; CAMPOS KEVIN R; TRUPPO MATTHEW; NAWRAT CHRISTOPHER C 权利人:MERCK SHARP & DOHME; MERCK SHARP & DOHME LLC 摘要:The present invention relates to an enzymatic synthesis of 4′-ethynyl-2′-deoxy nucleosides and analogs thereof, for example EFdA, that eliminates the use of protecting groups on the intermediates, improves the stereoselectivity of glycosylation and reduces the number of process steps needed to make said compounds. It also relates to the novel intermediates employed in the process.
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