CAS: 82473-24-3; 3-[(3-Cholamidopropyl)Dimethylammonio]-2-Hydroxy-1-Propanesulfonate

Sodium 3-Chloro-2-Hydroxypropane-Sulfonate,3-(胆酰胺基丙基)-1,1-二甲胺 以 乙醇,水 作为反应溶剂,化学反应 10.0H,以192 G的收率获得产物3-[(3-胆胺丙基)二甲基氨基]-2-羟基-1-丙磺酸内盐
参考文献:一种chapso的制备方法
标题:一种chapso的制备方法
摘要:本发明属于生化检测底物领域,涉及一种chapso的制备方法,步骤在于:将牛羊胆酸溶于乙腈,滴加氯甲酸乙酯反应2~3小时,得到溶液;溶液加入3‑二甲氨基丙胺,反应10~12小时,过滤,滤液减压浓缩为白色固体;将白色固体重结晶,得到3‑二甲氨基‑胆酰丙胺;将3‑二甲氨基‑胆酰丙胺溶于乙醇水溶液,加热至32°C,缓慢滴加3‑氯‑2‑羟基丙硫酸钠盐的水溶液,反应8~10小时,滤液减压浓缩得淡黄色粘稠物;将淡黄色粘稠物拌硅胶过柱,用甲醇:二氯甲烷梯度洗脱,收集纯品;将纯品真空干燥得到chapso.本发明所用的原料成本普遍较低,也没有毒副作用比较大的易挥发成分,收率能够达到60%以上,放大生产比较方便.

专利信息

专利号:US-2012190064-A1
优先权日:2004-10-01
标题 :Feeding buffers, systems, and methods for in vitro synthesis of biomolecules
发明人：KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA
权利人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA; LIFE TECHNOLOGIES CORP
摘要:Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of a feeding solution that includes amino acids and an energy source to an ongoing in vitro synthesis system. The invention also includes methods of using a high-yield in vitro synthesis system to produce large quantities of proteins with incorporated labeled amino acids for analysis by methods such as by NMR. The invention further includes vectors for enhanced production of proteins from nucleic acid templates using in vitro synthesis systems.

专利号:US-6451543-B1
优先权日:1998-08-31
标题:Lipid matrix-assisted chemical ligation and synthesis of membrane polypeptides
发明人：KOCHENDOERFER GERD G; HUNTER CHRISTIE L; KENT STEPHEN B H; BOTTI PAOLO
权利人:GRYPHON SCIENCES
摘要:The present invention relates to methods and compositions for lipid matrix-assisted chemical ligation and synthesis of membrane polypeptides that are incorporated in a lipid matrix. The invention is exemplified in production of a prefolded membrane polypeptide embedded within a lipid matrix via stepwise chemoselective chemical ligation of unprotected peptide segments, where at least one peptide segment is embedded in a lipid matrix. Any chemoselective reaction chemistry amenable for ligation of unprotected peptide segments can be employed. Suitable lipid matrices include liposomes, micelles, cell membrane patches and optically isotropic cubic lipidic phase matrices. Prefolded synthetic and semi-synthetic membrane polypeptides synthesized according to the methods and compositions of the invention also permit site-specific incorporation of one or more detectable moieties, such as a chromophore, which can be conveniently introduced during synthesis. The methods and compositions of the invention have multiple uses. For example, they can be used to assay ligand binding to membrane polypeptides and domains comprising a receptor, and thus are extremely useful for structure/function studies, drug screening/selection/design, and diagnostics and the like, including high-throughput applications. The methods and compositions of the invention are particularly suited for FRET analyses of previously inaccessible membrane polypeptides.

专利号:US-8067204-B2
优先权日:2005-12-15
标 题 :Long-chain chondroitin sugar chain and method for producing the same and method for promoting synthesis of chondroitin
发明人：SUGIURA NOBUO; SHIMOKATA SATOSHI; KIMATA KOJI
权利人:SUGIURA NOBUO; SHIMOKATA SATOSHI; KIMATA KOJI; SEIKAGAKU KOGYO CO LTD
摘要:A method for producing a chondroitin sugar chain comprises reacting a glucuronic acid donor, an N-acetyl galactosamine donor, a sugar receptor and a bacterial cell enzyme which synthesizes chondroitin in the presence of a surfactant. The surfactant is selected from polyoxyethylene octadecyl amine, n-decanoyl-N-methylglucamide, sodium cholate, n-octyl-β-D-thioglucopyranoside, n-nonyl-β-D-thiomaltopyranoside, sucrose monocholate, sucrose monocaprate, and sucrose monolaurate. The chondroitin sugar chain has all the following properties: a weight average molecular weight: 50,000 or more when measured by gel filtration chromatography; it is completely degraded to disaccharides with chondroitinase ABC; and when the sugar chain is decomposed with chondroitinase ABC and the decomposed products are subjected to a disaccharide analysis, substantially all of them correspond to an unsaturated disaccharide unit of chondroitin.

专利号:US-6713607-B2
优先权日:1994-03-08
标 题:Effector proteins of Rapamycin
发明人：CAGGIANO THOMAS J; CHEN YANQIU; FAILLI AMEDEO A; MOLNAR-KIMBER KATHERINE L; NAKANISHI KOJI
权利人:WYETH CORP; UNIV COLUMBIA
摘要:This invention comprises novel Rapamycin-FKBP12 binding proteins of mammalian origin for identification, design and synthesis of immunomodulatory, anti-restenosis or anti-tumor agents, as well as fragments of the proteins and the DNA, cDNA, antisense RNA and DNA segments corresponding to the proteins. This invention also comprises methods for isolating the proteins and therapeutic uses related to the proteins.

专利号:US-8226976-B2
优先权日:2007-11-05
标 题:Method of using cell-free protein synthesis to produce a membrane protein
发明人：YOKOYAMA SHIGEYUKI; SHIMONO KAZUMI; SHIROUZU MIKAKO; GOTO MIE
权利人:YOKOYAMA SHIGEYUKI; SHIMONO KAZUMI; SHIROUZU MIKAKO; GOTO MIE; RIKEN
摘要:A method is provided for producing a membrane protein folded to its native structure or active structure in a lipid disc or a liposome. The method comprises: (a) preparing a reaction solution for cell-free protein synthesis containing a polynucleotide encoding a membrane protein, a steroidal detergent, and a phospholipid, wherein the steroidal detergent is contained at a concentration higher than its critical micelle concentration; (b) decreasing the concentration of said steroidal detergent in the reaction solution; and (c) synthesizing the membrane protein simultaneously with formation of a lipid disk or liposome into which the synthesized membrane protein is integrated.

专利号:US-7482425-B2
优先权日:1999-08-26
标题:Compositions for lipid matrix-assisted chemical ligation
发明人：KOCHENDOERFER GERD G; HUNTER CHRISTIE L; KENT STEPHEN B H; BOTTI PAOLO
权利人:AMYLIN PHARMACEUTICALS INC
摘要:The present invention relates to methods and compositions for lipid matrix-assisted chemical ligation and synthesis of membrane polypeptides that are incorporated in a lipid matrix. The invention is exemplified in production of a prefolded membrane polypeptide embedded within a lipid matrix via stepwise chemoselective chemical ligation of unprotected peptide segments, where at least one peptide segment is embedded in a lipid matrix. Any chemoselective reaction chemistry amenable for ligation of unprotected peptide segments can be employed. Suitable lipid matrices include liposomes, micelles, cell membrane patches and optically isotropic cubic lipidic phase matrices. Prefolded synthetic and semi-synthetic membrane polypeptides synthesized according to the methods and compositions of the invention also permit site-specific incorporation of one or more detectable moieties, such as a chromophore, which can be conveniently introduced during synthesis. The methods and compositions of the invention have multiple uses. For example, they can be used to assay ligand binding to membrane polypeptides and domains comprising a receptor, and thus are extremely useful for structure/function studies, drug screening/selection/design, and diagnostics and the like, including high-throughput applications. The methods and compositions of the invention are particularly suited for FRET analyses of previously inaccessible membrane polypeptides.