专利号:US-2012190064-A1 优先权日:2004-10-01 标题 :Feeding buffers, systems, and methods for in vitro synthesis of biomolecules 发明人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA 权利人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA; LIFE TECHNOLOGIES CORP 摘要:Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of a feeding solution that includes amino acids and an energy source to an ongoing in vitro synthesis system. The invention also includes methods of using a high-yield in vitro synthesis system to produce large quantities of proteins with incorporated labeled amino acids for analysis by methods such as by NMR. The invention further includes vectors for enhanced production of proteins from nucleic acid templates using in vitro synthesis systems.
专利号:US-8110021-B2 优先权日:2008-07-28 标题:Synthesis of PtCo nanoparticles 发明人:ZHONG CHUAN-JIAN; LUO JIN; XU ZHICHAUN; HE TING 权利人:ZHONG CHUAN-JIAN; LUO JIN; XU ZHICHAUN; HE TING; HONDA MOTOR CO LTD; UNIV NEW YORK STATE RES FOUND 摘要:Synthesis of nanoparticles with particle size control is provided by the method of using two different metal-containing precursors, a capping component, an optional reducing agent, and then contacting the two precursors with the capping component to form a reaction solution, which is heated to produce first and second metals-containing nanoparticles. By controlling the ratio of the concentration of the capping component to the total concentration of the two metal-containing precursors, the nanoparticles can have diameters ranging between about 1 nm to about 15 nm. A decrease in the concentration of the capping component typically increases the size of the nanoparticles. Preferred compositions include Pt and Co-containing alloy nanoparticles. Controlled synthesis of larger, about 6 nm to about 12 nm, sized nanoparticles can be achieved in a solvent-free reaction process.
专利号:US-7713446-B2 优先权日:2004-06-28 标 题:Synthesis of nanofibers of polyaniline and substituted derivatives 发明人:EPSTEIN ARTHUR J; CHIOU NAN-RONG 权利人:OHIO STATE UNIVERISTY 摘要:Novel, simple methods are presented directed to the synthesis of nanofibers of polyaniline and substituted derivatives. The production of these fibers is achieved via various methods by controlling the concentration of aniline monomer or substituted aniline derivatives or an oxidant in the reaction medium and maintaining said concentration at a level much lower than conventional polyaniline synthesis methods. Methods are disclosed relating to the use of a permeable membrane to control the release of a monomer and/or oxidant as well as a bulk polymerization method.
专利号:US-2023287032-A1 优先权日:2020-08-14 标 题:Synthesis of fluorinated nucleotides 发明人:CHUNG CHEOL KEUN; LIU ZHIJIAN; MALIGRES PETER E; MAO EDNA; OBLIGACION JENNIFER V; PHILLIPS ERIC M; PIRNOT MICHAEL; POIRIER MARC; SONG ZHIGUO JAKE; WANG TAO 权利人:CHUNG CHEOL KEUN; LIU ZHIJIAN; MALIGRES PETER E; MAO EDNA; OBLIGACION JENNIFER V; PHILLIPS ERIC M; PIRNOT MICHAEL; POIRIER MARC; SONG ZHIGUO JAKE; WANG TAO; MERCK SHARP & DOHME LLC 摘要:The present invention relates to efficient processes useful in the preparation of fluorinated nucleosides, such as (O—{[(2R,3R,4S,5R)-5-(6-amino-9H-purin-9-yl)-4-fluoro-3-hydroxyoxolan-2-yl]methyl}O,O-dihydrogen phosphorothioate, also known as 2′-(S)-fluoro-thio-adenosine monophosphate or 2′-F-thio-AMP. Such fluorinated nucleosides may be useful as a biologically active compound and or as an intermediate for the synthesis of more complex biologically active compounds. The present invention also encompasses intermediates useful in the disclosed synthetic processes and the methods of their preparation.
专利号:US-11548838-B2 优先权日:2021-02-02 标 题:Process for synthesis of polyhydrocarbons as heat transfer agents 发明人:MANDAL TANMAY; VANKUDOTH KRISHNA; SARASWAT MANISHA; ARORA AJAY KUMAR; KAGDIYAL VIVEKANAND; SAXENA DEEPAK; RAMAKUMAR Sankara Sri Venkata 权利人:INDIAN OIL CORP LTD 摘要:The present invention provides a one-pot process of synthesis of phenyl naphthalene compounds that are employed as heat transfer agents. More particularly, the present invention provides a process of preparation of 1-phenylnaphthalene and 2-methyl-1-phenylnaphthalene using refinery spent catalyst. These molecules are known for application as synthetic heat transfer fluids that deliver outstanding performance and thermal stability at continuously high operating temperatures. The reaction is carried out in aqueous medium using a spent catalyst which is a palladium based charcoal catalyst as obtained from various refinery processes. Further, the present invention provides a heat resistant formulation using the synthesized polyhydrocarbons, wherein the formulation is optimized with a free radical scavenger.
专利号:US-7122304-B2 优先权日:1997-05-12 标 题:Methods for the storage and synthesis of nucleic acids using a solid support 发明人:GOLDSBOROUGH MINDY D; FOX DONNA K 权利人:WHATMAN INC 摘要:The invention relates to storage of nucleic acid (particularly mRNA) on a solid support and to using such nucleic acid in nucleic acid synthesis or amplification reactions. In a preferred aspect, the invention provides synthesis of cDNA and cDNA libraries.
1: Espinosa YR, Grigera RJ, Ferrara CG. Mechanisms associated with the effects of urea on the micellar structure of sodium dodecyl sulphate in aqueous solutions. Prog Biophys Mol Biol. 2018 May 11. pii: S0079-6107(17)30039-1. doi: 10.1016/j.pbiomolbio.2018.05.006. [Epub ahead of print] Review. doi: 10.1007/s12551-017-0266-6. Epub 2017 May 29. Review. 3: Barra Caracciolo A, Cardoni M, Pescatore T, Patrolecco L. Characteristics and environmental fate of the anionic surfactant sodium lauryl ether sulphate (SLES) used as the main component in foaming agents for mechanized tunnelling. Environ Pollut. 2017 Jul;226:94-103. doi: 10.1016/j.envpol.2017.04.008. Epub 2017 Apr 12. Review. doi: 10.1016/j.jes.2016.05.027. Epub 2016 Jul 9. Review. doi: 10.1016/j.jprot.2017.03.002. Epub 2017 Mar 8. Review. pii: E27. doi: 10.3390/proteomes4030027. Review. 7: Chen T, Chen Y, Stella C, Medley CD, Gruenhagen JA, Zhang K. Antibody-drug conjugate characterization by chromatographic and electrophoretic techniques. J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Oct 1;1032:39-50. doi: 10.1016/j.jchromb.2016.07.023. Epub 2016 Jul 14. Review. doi: 10.1016/j.pnmrs.2016.01.004. Epub 2016 Feb 4. Review. doi: 10.1111/pde.12819. Epub 2016 Feb 26. Review.
合成参考文献
摘要:U.S. Coast Guard. 1999. Chemical Hazard Response Information System (CHRIS) - Hazardous Chemical Data. Commandant Instruction 16465.12C. Washington, D.C.: U.S. Government Printing Office. 摘要:National Toxicology Program, Institute of Environmental Health Sciences, National Institutes of Health (NTP). 1992. National Toxicology Program Chemical Repository Database. Research Triangle Park, North Carolina.