7,8-Dihydro-8-Hydroxypalmatine置于sodium Hydroxide体系中,用 乙醇,氯仿,水 作为反应溶剂,化学反应 4.0H,以20%的收率获得产物黄藤素 参考文献:A 7,8-Dihydro-8-Hydroxypalmatine From Enantia Chlorantha 标题:A 7,8-Dihydro-8-Hydroxypalmatine From Enantia Chlorantha 摘要:7,8-Dihydro-8-Hydroxypalmatine,A Novel Protoberberine Type Alkaloid,Along With Palmatine,Has Been Isolated And Characterized From An Anti-Hiv Active Extract From Enantia Chlorantha. The Structures Of The Two Compounds Were Elucidated By Spectroscopic Analyses And From Chemical Evidence. (C) 1998 Elsevier Science Ltd. All Rights Reserved. DOI:10.1016/s0031-9422(98)00382-3
专利号:WO-2025031833-A1 优先权日:2023-08-04 标 题:Recombinant strain and use thereof in production of scoulerine 发明人:CHEN YUN; JIAO XIANG 权利人:CHRYSEA LTD; CHEN YUN 摘要:Disclosed are recombinant yeast strains and a use thereof in the production of a benzylisoquinoline alkaloid. The recombinant yeast strains comprise berberine bridge enzyme. De novo synthesis of scoulerine and a benzylisoquinoline alkaloid is achieved through multiple strategies, and the yield thereof is significantly increased. The recombinant yeast strains provided by the present invention can produce scoulerine and other benzylisoquinoline alkaloids at high efficiency, and has important value in applications.
专利号:US-8759480-B2 优先权日:2006-02-17 标题:Contoxin analogues and methods for synthesis of intramolecular dicarba bridge-containing peptides 发明人:ROBINSON ANDREA; ELARIDI JOMANA 权利人:ROBINSON ANDREA; ELARIDI JOMANA; SYNGENE LTD 摘要:According to the present invention, there is provided a range of new conotoxin derivatives and methods for synthesizing these analogues and other intramolecular dicarba bridge-containing peptides, including dicarba-disulfide bridge-containing peptides.
专利号:WO-2024121871-A1 优先权日:2022-12-09 标 题 :Genetic modification of microbes for production of nitrogen and carbon-containing compounds 发明人:KANUMURU RAHUL RAJU 权利人:FERTIS INDIA PVT LTD 摘要:The present invention relates to a novel method for sustainable in vitro and in vivo production of nitrogen and carbon containing compounds such as and not limited to Proteins, Enzymes, peptides amino acids, nucleotides, vitamins, etc. The present invention relates to a novel method that includes gene modifications in microbes, more specifically involving gene modifications resulting in nitrogen fixation and/or ammonia fixation or increased nitrogen fixation and/or ammonia fixation or carbon fixation/increased carbon fixation or Hydrogen fixation/increased Hydrogen fixation with increased synthesis/ regeneration of anyone or all compounds including ATP, NADH, NADPH, Pyruvate and Acetyl CoA with or without utilisation/reduction enhanced utilisation/reduction of Nitrate, Nitrite, N2O, N2O2 and/or carbon containing substrates
专利号:US-2009227470-A1 优先权日:2005-12-21 标题:Selection of biocatalysts for chemical synthesis 发明人:WITHOLT BERNARD; VAN BEILEN JAN BERTHOLD; VAN SINT FIET STEPHAN JOHANNES 权利人:WITHOLT BERNARD; VAN BEILEN JAN BERTHOLD; VAN SINT FIET STEPHAN JOHANNES 摘要:The present invention relates to a method of detecting among a population of candidate biocatalysts a biocatalyst capable of catalyzing a chemical conversion reaction from a substrate to a product, said method comprising the steps of: a) providing a host cell comprising:—at least one product-inducible expression system comprising nucleic acid encoding at least one detector gene operably linked to a regulatory element, wherein the expression of said detector gene is inducible by said product, and—at least one biocatalyst expression system comprising nucleic acid encoding at least one candidate biocatalyst, wherein said at least one candidate biocatalyst is selected from said population of candidate biocatalysts, b) contacting said host cell with said substrate under conditions wherein said nucleic acid encoding at least one candidate biocatalyst is expressed in said host cell and wherein said substrate is allowed to contact said candidate biocatalyst, and wherein said substrate is converted into said product in case said candidate biocatalyst is capable of catalyzing said reaction, and c) detecting said host cell as one comprising a biocatalyst capable of catalyzing said chemical conversion reaction on the basis of the expression of said detector gene.
专利号:US-2013123463-A1 优先权日:2006-02-17 标题:Methods for the synthesis of two or more dicarba bridges in organic compounds
专利号:US-2012142892-A1 优先权日:2006-02-17 标题:Contoxin analogues and methods for synthesis of intramolecular dicarba bridge-containing peptides
1: Long J, Song J, Zhong L, Liao Y, Liu L, Li X. Palmatine: A review of its pharmacology, toxicity and pharmacokinetics. Biochimie. 2019 Jul;162:176-184. doi: 10.1016/j.biochi.2019.04.008. Epub 2019 Apr 30. 105:76-85. doi: 10.1016/j.molimm.2018.10.015. Epub 2018 Nov 27. 14:4963-4974. doi: 10.2147/DDDT.S280520.
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