专利号:US-5429939-A 优先权日:1989-04-21 标题 :DNA sequences useful for the synthesis of carotenoids 发明人:MISAWA NORIHIKO; KOBAYASHI KAZUO; NAKAMURA KATSUMI; YAMANO SHIGEYUKI 权利人:KIRIN BREWERY 摘要:Disclosed are DNA sequences which are useful for the synthesis of carotenoids such as lycopene, β-carotene, zeaxanthin or zeaxanthin-diglucoside, that is, DNA sequences encoding carotenoid biosynthesis enzymes. These DNA sequences are the sequences 1-6 shown in the specification. n Also disclosed is a process for producing a carotenoid or a carotenoid related compound which is selected from the group consisting of geranylgeranyl pyrophosphate, phytoene, lycopene, β-carotene, zeaxanthin and zeaxanthin-diglucoside, which comprises transforming a host with at least one of the DNA sequences 1-6 described above and culturing the transformant.
专利号:US-5583030-A 优先权日:1992-06-30 标 题 :Method for obtaining antifungal and herbicidal compounds that target the first committed step in shingolipid long-chain base biosynthesis 发明人:DICKSON ROBERT C; LESTER ROBERT L 权利人:UNIV KENTUCKY RES FOUND 摘要:The invention provides the LCB1 and LCB2 genes of the yeast Saccharomyces cerevisiae that encode subunits of the enzyme serine palmitoyltransferase (SPT), the first enzyme leading to synthesis of the long-base component of the sphingolipids. The present specification describes the isolation of the LCB1 and LCB2 genes. The invention further relates to methods of using these genes to either inhibit SPT activity or to inhibit synthesis of the enzyme. Furthermore, the invention relates to methods for constructing strains of S. cerevisiae or other organisms that can be used to select and to test for compounds that either inhibit SPT activity or to inhibit synthesis of the enzyme.
专利号:US-5908975-A 优先权日:1993-11-09 标题:Accumulation of fructans in plants by targeted expression of bacterial levansucrase 发明人:CAIMI PERRY GERARD; HERSHEY HOWARD PAUL; KERR PHILLIP S 权利人:DU PONT 摘要:This invention concerns methods for synthesis and accumulation of fructose polymers in seed, tubers or leaves of transgenic plants by selective expression of a bacterial fructosyltransferase gene. Selective expression includes coordination of timing, tissue specific expression and especially subcellular location. Successful transformants utilize sucrose to synthesize and accumulate fructan in the vacuole of the cell, in established crops, without loss of co-products or concern for yield loss due to degradation during maturation, harvest or storage of the plant. Enhanced fructan production will benefit the fructose sweetener industry and add value to grain used for feed.
专利号:US-2013086712-A1 优先权日:2008-08-21 标题 :Nitrogen responsiveness in plants through the expression of pathways for the formation and catabolism of novel n-rich compounds 发明人:GUPTA RAJEEV; DHUGGA KANWARPAL SINGH 权利人:PIONEER HI BRED INT; PIONEER HI BRED INT 摘要:The invention provides for the regulation of opine synthesis and catabolism providing improved nitrogen responsiveness, utilizing opine synthase and oxidase nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering nitrogen utilization and/or uptake in plants. The invention further provides recombinant expression cassettes, host cells and transgenic plants.
专利号:US-2006030014-A1 优先权日:2004-08-03 标题:Synthesis of polyhydroxyalkanoates in the cytosol of yeast
专利号:US-8815566-B2 优先权日:2004-11-04 标题:High eicosapentaenoic acid producing strains of Yarrowia lipolytica 发明人:DAMUDE HOWARD GLENN; GILLIES PETER JOHN; MACOOL DANIEL JOSEPH; PICATAGGIO STEPHEN K; POLLAK DANA M WALTERS; RAGGHIANTI JAMES JOHN; XUE ZHIXIONG; YADAV NARENDRA S; ZHANG HONGXIANG; ZHU QUINN QUN 权利人:DU PONT 摘要:Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 25% eicosapentaenoic acid (EPA, an ω-3 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of EPA. Production host cells are claimed, as are methods for producing EPA within said host cells.