CAS: 1927-31-7; 2'-Deoxyadenosine-5'-Triphosphate

相似化合物

74299-50-6 653-63-4 72003-83-9

上下游产品

CAS号56-65-5 5'-三磷酸腺苷 | CAS号653-63-4 2'-脱氧腺苷-5'-单磷酸 | CAS号58-64-0 腺苷-5'-二磷酸 | CAS号958-09-8 2'-脱氧腺苷 | CAS号2793-06-8 [[(2R,3S,5R)-5-... | CAS号958-09-8 2'-脱氧腺苷

5'-三磷酸腺苷置于dl-Dithiothreitol,L. Leichmannii Ribonucleosidetriphosphate Reductase体系中,用 Various Solvent(S) 用作溶剂,化学反应 120.0H,以89%的收率获得2'-脱氧腺苷 5'-三磷酸酯
参考文献:Preparative 2'-Reduction Of Atp Catalyzed By Ribonucleotide Reductase Purified By Liquid-Liquid Extraction
标题:Preparative 2'-Reduction Of Atp Catalyzed By Ribonucleotide Reductase Purified By Liquid-Liquid Extraction
摘要:通过 Peg 4000(16%(w/w))/磷酸盐(7%(w/w))液液萃取一步法从超声提取的细胞材料中纯化了由大肠杆菌 Hb101 Psquire 组成型表达的重组雷氏乳杆菌核糖核苷三磷酸还原酶(rtpr,E.C.1.17.4.2).在该制备系统中,顶相的 Rtpr 产率高达 75.1%,顶相和底相的总 Rtpr 活性分配比例为 8.5:1.含 Rtpr 的顶相被用于还原 2'位的 Atp,最终转化率和产率都很高,证明核糖核苷酸还原酶方法可用于 2'-脱氧核糖核苷酸的制备合成.反应中高浓度的醋酸钠可替代 Rtpr 的异生效应物.1,4-二硫代-Dl-苏力糖醇被用作 Rtpr 的人工还原剂.
Doi:10.1271/bbb.64.1836

专利信息

专利号:US-2023235372-A1
优先权日:2020-06-12
标题:Ab-initio, template-independent synthesis of nucleic acids using thermostable enzymes
发明人：RANDRIANJATOVO-GBALOU IRINA; SAID AHMED; RAHIER RENAUD
权利人:SYNHELIX
摘要:The invention relates to the field of nucleic acid synthesis or sequencing, more specifically to methods for ab-initio synthesis of nucleic acids, comprising contacting a nucleotide with a free 3′-hydroxyl group, with at least one nucleoside triphosphate, or a combination of nucleoside triphosphates, in the presence of an archaeal DNA primase or a functionally active fragment and/or variant thereof, thereby covalently binding said nucleoside triphosphate to the free 3′-hydroxyl group of the nucleotide. It also relates to isolated functionally active fragments of archaeal DNA primases which are capable of both ab-initio single-stranded nucleic acid synthesis activity and template-independent terminal nucleotidyl transferase activity.

专利号:US-10501782-B1
优先权日:2014-09-05
标题:Cell-free synthesis of DNA by strand displacement
发明人：PORTER NEIL; ROTHWELL PAUL; EXTANCE JONATHAN
权利人:TOUCHLIGHT IP LTD
摘要:The present invention relates to an improved process for synthesis of DNA, RNA, proteins and like molecules, in particular cell-free enzymatic synthesis of DNA, preferably in large scale. The present invention relates to the synthesis of DNA using strand-displacement replication and the addition of nucleotides to the reaction mixture is controlled, thus controlling yield. The reaction mixture contains a starting amount of nucleotides, polymerase and DNA template, to which further nucleotides are supplied in a controlled manner.

专利号:US-2023313255-A1
优先权日:2020-07-15
标题:Massively Parallel Enzymatic Synthesis of Polynucleotides
发明人：HORGAN ADRIAN; LACHAIZE HENRI; VERARDO DOMIANO; GODRON XAVIER
权利人:DNA SCRIPT
摘要:The invention is directed to methods and compositions for inkjet assisted synthesis of a plurality of polynucleotides at reaction sites on a substrate using template-free polymerases, such as, terminal deoxynucleotidyl transferases (TdTs). Compositions of the invention include formulations of synthesis reagents for inkjet delivery including, but not limited to, TdT coupling reaction buffers and 3′-O-protected dNTP monomers.

专利号:US-7344863-B2
优先权日:1998-03-13
标题:Methods for producing polypeptides through enhanced synthesis of encoding nucleic acid molecules
发明人：LI WU-BO; JESSEE JOEL A; SCHUSTER DAVID; XIA JIULIN; GEBEYEHU GULILAT
权利人:INVITROGEN CORP
摘要:The present invention is directed to compositions and methods for enhancing synthesis of nucleic acid molecules, particularly GC-rich nucleic acid molecules. Specifically, the invention provides compositions comprising one or more nitrogen-containing organic compounds having a formula selected from the group consisting of formula I and formula II (or salts or derivatives thereof), preferably 4-methylmorpholine N-oxide or betaine (carboxymethyltrimethylammonium), and further comprising one or more compounds selected from the group consisting of proline and an N-alkylimidazole compound, and more preferably proline, 1-methylimidazole or 4-methylimidazole. The invention further relates to methods for enhanced, high-fidelity synthesis of nucleic acid molecules, including via amplification (particularly PCR), reverse transcription, and sequencing methods. The invention also relates to nucleic acid molecules synthesized by these methods, to fragments or derivatives thereof, and to vectors and host cells comprising such nucleic acid molecules, fragments, or derivatives. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the compositions of the invention.

专利号:US-12421628-B2
优先权日:2018-07-23
标题:Massively parallel enzymatic synthesis of nucleic acid strands
发明人：HORGAN ADRIAN; GODRON XAVIER; YBERT THOMAS; NICOL ROBERT
权利人:DNA SCRIPT
摘要:The invention is directed to methods for massively parallel template-free enzymatic synthesis of a plurality of different polynucleotides of predetermined sequences. In one aspect, methods of the invention employ large scale arrays of reaction sites each associated with at least one working electrode for controlling deprotection and deblocking steps at predetermined user selected sites. In another aspect, the invention provides template-free enzymatic synthesis with proofreading, wherein completed polynucleotides at predetermined reaction sites are sequenced using a sequencing by synthesis technique, particularly employing electrochemically labile blocking groups.

专利号:US-6576447-B2
优先权日:2000-03-21
标 题:Method for synthesis of nucleic acids
发明人：TONOIKE HIROSHI
权利人:SHIMADZU CORP
摘要:An object of the present invention is to provide a novel method for suppressing the action of nucleic acid synthesis inhibitory substances and thereby amplifying a nucleic acid in a sample efficiently.According to the present invention, in a method for synthesis of nucleic acids to amplify an intended nucleic acid in a sample, the sample is brought in advance into contact with an insoluble polymer of a polyanion, a sulfated polymer or a sulfated polysaccharide, to remove nucleic acid synthesis inhibitory substances.