专利号:US-10501782-B1 优先权日:2014-09-05 标题:Cell-free synthesis of DNA by strand displacement 发明人:PORTER NEIL; ROTHWELL PAUL; EXTANCE JONATHAN 权利人:TOUCHLIGHT IP LTD 摘要:The present invention relates to an improved process for synthesis of DNA, RNA, proteins and like molecules, in particular cell-free enzymatic synthesis of DNA, preferably in large scale. The present invention relates to the synthesis of DNA using strand-displacement replication and the addition of nucleotides to the reaction mixture is controlled, thus controlling yield. The reaction mixture contains a starting amount of nucleotides, polymerase and DNA template, to which further nucleotides are supplied in a controlled manner.
专利号:US-8420848-B2 优先权日:2009-01-09 标题:Process for the synthesis of beta glycerol phosphate 发明人:KULKARNI YASHWANT 权利人:KULKARNI YASHWANT; SIGMA ALDRICH CO LLC 摘要:The present invention provides methods for the preparation of beta glycerol phosphate and its salts. In particular, the invention provides efficient methods for the synthesis of beta glycerol phosphate of high purity.
专利号:US-2023091493-A1 优先权日:2020-02-14 标 题:Dna synthesis yield improvements 发明人:ROTHWELL PAUL; KYSH DANIEL; PORTER NEIL 权利人:TOUCHLIGHT IP LTD 摘要:The present invention relates to an improved process for synthesis of deoxyribonucleic acid (DNA), in particular cell-free enzymatic synthesis of DNA, preferably on a large or industrial scale, with an improved yield and/or with an improved efficiency. The invention requires the use of nucleotide complexes wherein the nucleotide is associated with a mixture of divalent and monovalent cations. Preferably, the divalent cation may be magnesium or manganese.
专利号:US-2022233673-A1 优先权日:2019-06-04 标 题:METHODS OF PRODUCING SHIGA TOXIN B-SUBUNIT (STxB) MONOMERS AND OLIGOMERS, AND USES THEREOF 发明人:BILLET ANNE; SCHMIDT FRÉDÉRIC; JOHANNES LUDGER; SERVENT DENIS; MOURIER GILLES; TARTOUR ÉRIC; KAY MICHAEL; FULCHER JAMES M 权利人:INST CURIE; CENTRE NAT RECH SCIENT; INST NAT SANTE RECH MED; COMMISSARIAT A LENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES CEA; APHP ASSIST PUBLIQUE HOPITAUX DE PARIS; UNIV PARIS; UNIV OF UTAH RESEARCH FOUDATION; UNIV UTAH RES FOUND 摘要:A method of producing a monomer of a Shiga toxin B-subunit (STxB) protein or of a variant thereof by peptide chemical synthesis, as well as to a method of producing a pentamer of the STxB protein or of the variant thereof. The methods are particularly advantageous as they overcome major issues typically observed in peptide chemical synthesis, including solubility and purity issues.
专利号:US-9657030-B2 优先权日:2010-06-11 标 题 :Transition metal-catalyzed processes for the preparation of N-allyl compounds and use thereof 发明人:GIGUERE JOSHUA ROBERT; MCCARTHY KEITH EDWARD; REISCH HELGE ALFRED; SANDOVAL SERGIO; STYMIEST JAKE LARRY 权利人:RHODES TECH 摘要:The present disclosure provides processes for the N-dealkylation of tertiary amines and the use of transition metal catalysts to prepare tertiary N-allyl amine derivatives and secondary amine derivatives thereof. The tertiary amines can be alkaloids and, more particularly, the tertiary amines can be opioids. In specific embodiments, the present disclosure provides methods for use in processes for the synthesis of naloxone and naltrexone from oripavine.
专利号:US-5047563-A 优先权日:1987-09-10 标题:Chiral crown ethers 发明人:DENTON JAMES B; YIP KIN-FAI 权利人:MILES INC 摘要:The synthesis and use of optically active isomers of bis(12-crown-4-methyl) dialkylmalonates as sodium specific ionophores is described. The synthesis of optically active isomers of hydroxymethyl 12-crown-4 useful in synthesizing ionophores is also described.
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