CAS: 113231-05-3; (S)-2,2'-((5-Amino-1-Carboxypentyl)Azanediyl)Diacetic Acid

相似化合物

113231-04-2 5746-04-3 1030283-84-1

上下游产品

CAS号113231-04-2 (S)-2,2'-((5-((... | CAS号205379-08-4 N-(5-氨基-1-羧基戊基)... | CAS号1155-64-2 Nε-苄氧羰基-L-赖氨酸 | CAS号205379-07-3 N2,N2-Bis[2-(1,... | CAS号113231-04-2 (S)-2,2'-((5-((...

N-(5-氨基-1-羧基戊基)亚胺二乙酸三-T-丁酯置于三氟乙酸 Ethandithiol,三异丙基硅烷,苯酚体系中,化学反应 4.0H,反应生成N,N-双(羧甲基)-L-赖氨酸
参考文献:用于组氨酸标签蛋白的可切换识别的高亲和力接头
标题:用于组氨酸标签蛋白的可切换识别的高亲和力接头
摘要:我们渴望创建化学识别单元,以高亲和力和稳定性结合寡聚组氨酸标签,作为将光谱探针和其他功能元件选择性地连接到重组蛋白的工具.合成了几个包含 2-4 个次氮基三乙酸 (Nta) 部分的超分子实体,其中还包含一个氨基,荧光素作为灵敏的报告探针与之偶联.这些多价螯合剂头 (Mch)(称为 Bis-,Tris-和 Tetrakis-Nta)的特征在于它们与六组氨酸 (H6) 和十组氨酸 (H10) 标记目标的相互作用.通过分析尺寸排阻色谱观测到随着nta部分数量的增加,结合稳定性显着增加.通过等温滴定量热法测定的结合焓几乎随着螯合剂头和标签之间可能的配位键的数量增加而增加.然而,要获得完全相加的结合焓,需要寡聚组氨酸标签中大量过量的组氨酸.与单-Nta 相比,通过荧光去猝灭测量的 Mch/寡聚组氨酸复合物的解离动力学显示稳定性增加了 4 个数量级,并且对 Tris-Nta 达到了亚纳摩尔亲和力.随着
Doi:10.1021/ja050690C

专利信息

专利号:US-10400236-B2
优先权日:2013-08-30
标题 :Method of manufacturing protein array or peptide array, method of identifying functional protein or functional peptide, protein array or peptide array, and functional protein or functional peptide identification kit
发明人：ICHIKI TAKANORI; UENO SHINGO; OSAWA HISAO
权利人:UNIV TOKYO; NIKON CORP
摘要:There is provided a method of manufacturing a protein array or peptide array suitable for an efficient screening of a functional protein or functional peptide. The method of manufacturing a protein array or peptide array includes the steps of: (a) preparing a nucleic acid immobilized on a solid support and a cell-free synthesis system in a reactor, in which a reactor array includes the reactor having a specific aperture shape and a protein capture molecule or a peptide capture molecule provided on at least a portion of wall surface and bottom surface in the reactor; and (c) synthesizing a protein or peptide from the nucleic acid using the cell-free synthesis system and immobilizing the protein or peptide in the reactor.

专利号:CN-116891417-A
优先权日:2023-09-11
标 题:A kind of N,N-bis(carboxymethyl)-L-lysine synthesis method