专利号:US-2012190064-A1 优先权日:2004-10-01 标题 :Feeding buffers, systems, and methods for in vitro synthesis of biomolecules 发明人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA 权利人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA; LIFE TECHNOLOGIES CORP 摘要:Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of a feeding solution that includes amino acids and an energy source to an ongoing in vitro synthesis system. The invention also includes methods of using a high-yield in vitro synthesis system to produce large quantities of proteins with incorporated labeled amino acids for analysis by methods such as by NMR. The invention further includes vectors for enhanced production of proteins from nucleic acid templates using in vitro synthesis systems.
专利号:WO-2004011474-A1 优先权日:2002-07-31 标题:Universal support media for synthesis of oligomeric compounds 发明人:GUZAEV ANDREI P; MANOHARAN MUTHIAH; RAVIKUMAR VASULINGA T; KUMAR RAJU K 权利人:ISIS PHARMACEUTICALS INC; GUZAEV ANDREI P; MANOHARAN MUTHIAH; RAVIKUMAR VASULINGA T; KUMAR RAJU K 摘要:Compounds for the synthesis of oligomeric compounds, particularly oligonucleotide and oligonucleotide mimetics, are provided. In addition, methods for functionalizing a support medium with a first monomeric subunit and methods for the synthesis of oligomeric compounds utilizing the novel compounds bound to support media are provided.
专利号:US-6653468-B1 优先权日:2002-07-31 标 题 :Universal support media for synthesis of oligomeric compounds 发明人:GUZAEV ANDREI P; MANOHARAN MUTHIAH 权利人:ISIS PHARMACEUTICALS INC 摘要:Compounds for the synthesis of oligomeric compounds, particularly oligonucleotides and oligonucleotide mimetics, are provided. In addition, methods for functionalizing a support medium with a first monomeric subunit and methods for the synthesis of oligomeric compounds utilizing the novel compounds bound to support media are provided.
专利号:US-2009209032-A1 优先权日:2004-10-01 标 题 :Feeding buffers, systems, and methods for in vitro synthesis of biomolecules
专利号:US-2025065301-A1 优先权日:2022-05-16 标题:Mass Production of Multivariate Metal-Organic Frameworks for Water Harvesting 发明人:YAGHI OMAR M; HANIKEL NIKITA; ZHENG ZHILING 权利人:UNIV CALIFORNIA 摘要:Multivariate metal-organic framework (MTV-MOF) composition are synthesized in a hydrothermal reaction between a metal and deprotonated linkers mixed at different ratios in a relatively rapid, facile, scalable, and high-yield synthesis method of the MTV-MOF on a kilogram-scale.
专利号:WO-2006039622-A2 优先权日:2004-10-01 标 题 :Feeding buffers, systems, and methods for in vitro synthesis of biomolecules
参考文献:10.1186/1476-511x-10-27|10.1186/1476-511x-10-4 摘要:Karray A, Frikha F, Ben Ali Y, Gargouri Y, Bezzine S. Purification and biochemical characterization of a secreted group IIA chicken intestinal phospholipase A2. Lipids in Health and Disease. 2011 Feb 01;10(1):27. doi: 10.1186/1476-511x-10-27.