专利号:US-10059935-B2 优先权日:2015-12-17 标 题 :Composite of Paracoccus denitrificans immobilized on modified graphene oxide and its preparation method and application 发明人:LU JIANMEI; CHEN DONGYUN 权利人:UNIV SOOCHOW 摘要:This invention provided a preparation method of the composite which was immobilized Paracoccus denitrificans on modified graphene oxide and its application. The composite was obtained by following the steps below: 1) Synthesis of graphene oxide; 2) Synthesis of modified graphene oxide; 3) Acclimatization and immobilization of Paracoccus denitrificans. In this invention, the raw materials were low-cost and easily obtained used in the preparation process; easy operation, convenient, and no expensive instruments during the whole process; this invention of the composite could remove DMF from wastewater completely, and with the advantages of high efficiency, good recycle performance, economical, environmentally friendly, better feasibility.
专利号:WO-2020130862-A1 优先权日:2018-12-17 标题:Lyophilized fertilizer composition comprising iron siderophore chelates, lyophilized composition comprising siderophores, their processes of obtention and uses to treat plants 发明人:MONTEIRO SOARES HELENA MARIA VIEIRA; HENRIQUES FERREIRA CARLOS MIGUEL; VALENTE SOARES EDUARDO JORGE; LUCENA MAROTTA JUAN JOSÉ; LOPÉZ RAYO SANDRA 权利人:UNIV DO PORTO; INST SUPERIOR DE ENGENHARIA DO PORTO; UNIV MADRID AUTONOMA 摘要:Lyophilized fertilizer composition comprising iron siderophore chelates and a stabilizing agent and a lyophilized composition comprising siderophores and a stabilizing agent, wherein the stabilizing agent is starch. The invention further relates to processes of obtaining a lyophilized fertilizer composition and a lyophilized composition comprising a step of synthesis of at least one siderophore compound by means of biochemical transformation performed by at least one microorganism, and to the use of the compositions for treating cultivated plants, for example in conditions of low iron availability, wherein the compositions are capable of correcting chlorosis in plants and do not present environmental risks. The lyophilized fertilizer composition and the lyophilized composition have high storage stability.
专利号:WO-2023056699-A1 优先权日:2021-10-08 标题 :Genetically engineered strain capable of producing l-alanine, construction method therefor, and application thereof 发明人:XU PING; HAN XIAO; TAO FEI; MU XIAOLING; CHEN SIHONG; LI WEILI 权利人:ANHUI BBCA BIOCHEMICAL CO LTD 摘要:Disclosed are a genetically engineered strain capable of producing L-alanine, a construction method therefor, and an application thereof. A genetically engineered strain capable of efficiently producing alanine at a high temperature of 42°C to 55°C can be constructed by enhancing a glycolysis pathway or/and introducing a thermostable alanine dehydrogenase gene. The optical purity of L-alanine can further be significantly improved by knocking out an alanine racemase gene. When a lactic acid synthesis pathway exists in starting bacteria, the proportion of a pyruvic acid synthesis pathway can be increased by blocking the lactic acid synthesis pathway, so as to further improve the yield of L-alanine, such that high-temperature fermentation production of L-alanine at 42°C-55°C is achieved, and the yield of alanine can reach more than 95 g/L.
专利号:US-2025277225-A1 优先权日:2021-10-08 标题:DL-Alanine-Producing Genetically Engineered Strain and Method of Construction and Use Thereof 发明人:TAO FEI; HAN XIAO; XU PING; MU XIAOLING; LI WEILI; CHEN SIHONG 权利人:ANHUI BBCA BIOCHEMICAL CO LTD 摘要:The present invention discloses a DL-alanine-producing genetically engineered strain, as well as a method of construction and use thereof, and pertains to the field of bioengineering. According to the present invention, through enhancing the glycolysis pathway or/and introducing thermostable alanine dehydrogenase, a genetically engineered strain capable of high-yield production of alanine at 42° C. to 55° C. This strain can be used in a two-step method for producing racemic DL-alanine, which includes fermentation and subsequent addition of microbial alanine racemase. Through inactivating or deleting alanine racemase genes in this strain and then separately introducing overexpressed alanine racemase gene(s), a genetically engineered strain capable of producing racemic DL-alanine using a direct fermentation method can be constructed. When the original strain possesses a lactate synthesis pathway, blocking this lactate synthesis pathway in both the genetically engineered strains can additionally augment the proportion of a pyruvate synthesis pathway.
专利号:US-10287592-B2 优先权日:2014-02-07 标 题:Bacillus host 发明人:VAN RIJ EVERT TJEERD; SPAANS MARTINE; HILLEBRAND HENDRIKUS BERNARDUS CAROLUS 权利人:DSM IP ASSETS BV 摘要:The invention provides Bacillus host cell deficient in the production of a neutral protease and of a alkaline protease which host cell comprises a nucleic acid construct, wherein the nucleic acid construct comprises a polynucleotide encoding a compound of interest or encoding a compound involved in the synthesis of a compound of interest operably linked to a promoter sequence allowing for expression of the polynucleotide in the host cell, wherein the promoter comprises a bacteriophage promoter sequence, more preferably a bacteriophage SPO1 promoter sequence. Said host cell can advantageously be used in a method of production of a compound of interest.
专利号:CN-119931908-B 优先权日:2025-04-10 标 题 :A spherical red bacteria and its application in de novo synthesis of oxidized coenzyme Q10
参考文献:10.1186/s13567-015-0268-y 摘要:Tassi R, McNeilly TN, Sipka A, Zadoks RN. Correlation of hypothetical virulence traits of two Streptococcus uberis strains with the clinical manifestation of bovine mastitis. Veterinary Research. 2015 Oct 23;46(1):123. doi: 10.1186/s13567-015-0268-y. 摘要:Biochemical Pharmacology., 15(1691), 1966