专利号:US-2012190064-A1 优先权日:2004-10-01 标题 :Feeding buffers, systems, and methods for in vitro synthesis of biomolecules 发明人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA 权利人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA; LIFE TECHNOLOGIES CORP 摘要:Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of a feeding solution that includes amino acids and an energy source to an ongoing in vitro synthesis system. The invention also includes methods of using a high-yield in vitro synthesis system to produce large quantities of proteins with incorporated labeled amino acids for analysis by methods such as by NMR. The invention further includes vectors for enhanced production of proteins from nucleic acid templates using in vitro synthesis systems.
专利号:US-2009209032-A1 优先权日:2004-10-01 标 题 :Feeding buffers, systems, and methods for in vitro synthesis of biomolecules
专利号:US-2006110788-A1 优先权日:2004-10-01 标题 :Feeding buffers, systems, and methods for in vitro synthesis of biomolecules
专利号:US-2010099150-A1 优先权日:2007-03-06 标题 :Polymerase stabilization by ionic detergents 发明人:FANG NAN; LOEFFERT DIRK; ERBACHER CHRISTOPH; PETERS LARS-ERIK 权利人:QIAGEN GMBH 摘要:The invention relates to a composition comprising (i) an enzyme with nucleic acid polymerase activity, (ii) an inert protein and, (ii) a zwitterionic detergent. The invention also relates to a composition comprising (i) an enzyme with nucleic acid polymerase activity, (ii) an inert protein and, (ii) a zwitterionic detergent. The invention further relates to a method for enzymatic nucleic acid synthesis comprising the steps of, (a) providing in a reaction mixture, a polymerase activity, a nucleic acid template, a zwitterionic detergent, a buffer, a salt, nucleotides and an inert protein and, (b) incubating the reaction mixture at a temperature which enables nucleic acid synthesis.
参考文献:10.1007/978-981-15-6637-0_13 摘要:Gu K, Yang M, Peng H. Strategies Towards Improving the Stability of All-Inorganic Perovskite Quantum Dots. 2020. In: Springer Series in Materials Science. 参考文献:10.1038/s41566-023-01225-w 摘要:Kaplan AEK, Krajewska CJ, Proppe AH, Sun W, Sverko T, Berkinsky DB, Utzat H, Bawendi MG. Hong–Ou–Mandel interference in colloidal CsPbBr3 perovskite nanocrystals. Nat. Photon. 2023 Jun 22;17(9):775–80. doi: 10.1038/s41566-023-01225-w. 参考文献:10.1007/978-3-031-54779-9_14 摘要:Sharma KH, Sharma N, Srivastava C. Quantum Dots Composites in Catalysis Applications. 2024. In: Engineering Materials. 参考文献:10.1007/978-981-97-1347-9_4 摘要:Zang Z, Zhao S, Cai W, Wang H. Inorganic Perovskite Semiconductors Laser. 2024. In: Springer Series in Materials Science.