专利号:US-2023097824-A1 优先权日:2020-05-08 标 题:Semi-synthesis and use of racemic hematoxylin 发明人:DURRANT EDWARD E; HEINDL DIETER; HUBER FLORIAN MAURITIUS ERASUMU; KLEIN ERIC; KOSMEDER II JEROME W; VOSS EDGAR 权利人:VENTANA MED SYST INC; ROCHE DIAGNOSTICS OPERATIONS INC 摘要:A racemic hematoxylin formulation is disclosed that includes one or both of a stabilizer compound and an antioxidant. The disclosed composition exhibits sufficient stability to be utilized in an automated staining process. Methods of using and making the stabilized composition also are disclosed.
专利号:US-2003186237-A1 优先权日:2001-02-14 标题:Methods and compositions of amplifying RNA 发明人:GINSBERG STEPHEN D; CHE SHAOLI 权利人:BAYLOR COLLEGE MEDICINE 摘要:The present invention pertains to a method that will increase the efficiency of second strand cDNA synthesis through a mechanism of “terminal continuationâ€? before further RNA amplification by RNA transcription using, for example, a bacteriophage promoter. In a specific embodiment, a transcription promoter is attached to the 5′ region of cDNA through the same mechanism of “terminal continuationâ€?. Genetic signals are subsequently amplified in a linear manner through RNA transcription. In specific embodiments, the orientation of the transcribed RNA is either sense or antisense, depending on the desired downstream application. In other embodiments, the present invention pertains to methods for extraction and amplification of RNA, particularly mRNA, from histologically stained tissues and cells.
专利号:EP-4356350-A1 优先权日:2021-06-17 标题:Digital synthesis of histological stains using multiplexed immunofluorescence imaging
专利号:US-2023081381-A1 优先权日:2020-02-20 标题 :METHODS TO COMBINE FIRST AND SECOND STRAND cDNA SYNTHESIS FOR SPATIAL ANALYSIS 发明人:CHEW JENNIFER 权利人:10X GENOMICS INC 摘要:Provided herein are methods of identifying the spatial location of a nucleic acid in a biological sample. In some embodiments, the methods employ a template switching oligonucleotide. In some embodiments, the methods extend the capture probe to create a complementary DNA (cDNA) molecule of a capture analyte and produce second strand in one reaction.
专利号:CN-117529750-A 优先权日:2021-06-17 标题 :Digital synthesis of histological staining using multiplex immunofluorescence imaging
专利号:US-12435312-B2 优先权日:2022-02-28 标 题 :Quantifying cell-derived changes in collagen synthesis, alignment, and mechanics in a 3D connective tissue model 发明人:MORGAN JEFFREY R; WILKS BENJAMIN T 权利人:UNIV BROWN 摘要:Described herein are in vitro quantitative methods directed to 3D extracellular matrix (ECM) organization and mechanics. The methods do not require application of an external force, anchorage, or a scaffolding material in order to culture ECM tissue constructs, and the methods enable quantitative measurements without crosstalk noise from variables attributable to external forces, anchorage, and scaffolding material.
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