专利号:US-10501782-B1 优先权日:2014-09-05 标题:Cell-free synthesis of DNA by strand displacement 发明人:PORTER NEIL; ROTHWELL PAUL; EXTANCE JONATHAN 权利人:TOUCHLIGHT IP LTD 摘要:The present invention relates to an improved process for synthesis of DNA, RNA, proteins and like molecules, in particular cell-free enzymatic synthesis of DNA, preferably in large scale. The present invention relates to the synthesis of DNA using strand-displacement replication and the addition of nucleotides to the reaction mixture is controlled, thus controlling yield. The reaction mixture contains a starting amount of nucleotides, polymerase and DNA template, to which further nucleotides are supplied in a controlled manner.
专利号:US-2025136636-A1 优先权日:2021-08-16 标 题:Nucleotide complexes capable of an improved dna synthesis yield 发明人:KYSH DANIEL; ROTHWELL PAUL 权利人:TOUCHLIGHT IP LTD 摘要:The present invention relates to novel nucleotide complexes which enhance the enzymatic production of DNA. The nucleotide complexes includes a divalent cation at a ratio of 0.2-1.5 divalent cations per nucleotide, whilst monovalent ions (other than hydrogen or hydronium ions from any solvent) are minimal. The nucleotide complexes have desirable properties for the synthesis of deoxyribonucleic acid (DNA), in particular cell-free enzymatic synthesis of DNA, preferably on a large or industrial scale. Further, the invention includes improved processes for preparing said nucleotide complexes. Further complexes are disclosed which possess unique properties in accelerating DNA synthesis at higher concentrations, these complexes also containing zwitterion molecules.
专利号:US-2023091493-A1 优先权日:2020-02-14 标 题:Dna synthesis yield improvements 发明人:ROTHWELL PAUL; KYSH DANIEL; PORTER NEIL 权利人:TOUCHLIGHT IP LTD 摘要:The present invention relates to an improved process for synthesis of deoxyribonucleic acid (DNA), in particular cell-free enzymatic synthesis of DNA, preferably on a large or industrial scale, with an improved yield and/or with an improved efficiency. The invention requires the use of nucleotide complexes wherein the nucleotide is associated with a mixture of divalent and monovalent cations. Preferably, the divalent cation may be magnesium or manganese.
专利号:US-2024309142-A1 优先权日:2023-03-17 标题 :Capped block copolymers, their synthesis, manufacture, and methods of use 发明人:MAN VICTOR FUK-PONG; LI XIAOJIN HARRY; CHEN YIQING; PU GANG; HUNT JR CLINTON 权利人:ECOLAB USA INC 摘要:This disclosure relates to capped block copolymers, preferably reverse block copolymers capped with a hydrophobic group. Preferred hydrophobic groups include benzyl groups and silyl groups. In a preferred embodiment, the reverse block copolymer has at least two arms, wherein each arm is capped with a hydrophobic group. The disclosure also provides for the synthesis of the capped block copolymers as well as methods of using the capped block copolymers for defoaming and antifoaming applications.
专利号:US-6022977-A 优先权日:1997-03-26 标 题 :Dynamic resolution of isoxazoline thioesters to isoxazoline carboxylic acids 发明人:ZHANG LIN-HUA; ANZALONE LUIGI; PESTI JAAN A; YIN JIANGUO 权利人:DU PONT PHARM CO 摘要:The present invention relates generally to a novel method for preparation of substituted isoxazolin-5-yl acetic acid in high optical purity from a stereoisomeric mixture of an esterified substituted isoxazolin-5-yl acetate. The products are useful in the synthesis of compounds for pharmaceuticals, especially the treatment of thrombolytic disorders, and agricultural products.
专利号:US-5916750-A 优先权日:1997-01-08 标 题 :Multifunctional linking reagents for synthesis of branched oligomers 发明人:IYER RAJKUMAR SIVA; SU SHENG-HUI; INAMDAR ANITA; KALRA KRISHAN L 权利人:BIOGENEX LAB 摘要:Reagents capable of forming branched oligomers with monomeric units are disclosed, together with oligomers incorporating such reagents, kits containing such reagents and methods for use of such reagents in forming oligomers with monomeric units. The present reagents can advantageously be used to introduce multiple labels or reporter molecules onto oligomers such as oligonucleotides and oligopeptides. In particular, non-nucleosidic phosphoramidites based on 1,3,5-tris(2-hydroxyethyl)cyanuric acid are disclosed. Multiply-labeled, branched DNA oligomer probes constructed using these phosphoramidite reagents showed increased signal intensity relative to singly-labeled oligomer probes.
[参考文献]: Ryoheigotoh, Et Al. Controlling Heat Release Of Crystallization From Supercooling State Of A Solid-Solid Pcm, 2-Amino-2-Methyl-1,3-Propanediol. International Journal Of Heat And Mass Transfer, 2019, 1132-1140. [参考文献]: A A Lemmens, Et Al. Isotachophoresis As A Candidate Reference Method In Analytical Chemistry. Determination Of Sodium In Serum. J Chromatogr. 1985 Feb 22;320(1):193-7. [参考文献]: Christina L Burnett, Et Al. Final Amended Report On Safety Assessment On Aminomethyl Propanol And Aminomethyl Propanediol. Int J Toxicol. 2009 Nov-Dec;28(6 Suppl):141S-61S. [参考文献]: Guangye Wang, Et Al. Gliotoxin Potentiates Osteoblast Differentiation By Inhibiting Nuclear Factor-κb Signaling. Mol Med Rep. 2015 Jul;12(1):877-84. [参考文献]: Joseph Chamieh, Et Al. Generalized Polymer Effective Charge Measurement By Capillary Isotachophoresis. J Chromatogr A. 2014 Nov 28:1370:255-62.
合成参考文献
摘要:Archives of Environmental Contamination and Toxicology., 14(111), 1985 [] 摘要:S. Glasstone and A. F. Schram. J. Am. Chem. Soc. 69, 1213 (1947). 摘要:S13 | EUCOSMETICS | Combined Inventory of Ingredients Employed in Cosmetic Products (2000) and Revised Inventory (2006) | DOI:10.5281/zenodo.2624118 摘要:Schafer, E. W., Jr., W. A. Bowles Jr., and J. Hurlbut. 1983. The acute oral toxicity and repellency and hazard potential of 998 chemicals to one or more species of wild and domestic birds. Archives of Environmental Contamination and Toxicology 12:355-382. 摘要:Schafer, E. W., Jr., and W. A. Bowles Jr. 2004. Toxicity, Repellency of Phytotoxicity of 979 Chemicals to Birds, Mammals and Plants. Research Report No. 04-01. National Wildlife Research Center, Fort Collins, Colorado. 118 pp. https://www.aphis.usda.gov/ws/nwrc/chem-effects-db/U_schafer041_2004.pdf