D-(+)-纤维四糖置于cellulosimicrobium Cellulans Strain 21 Gh1 β-Glucosidase Ccbgl1B,Recombinant,Molecular Weight: 57 Kda,水体系中,用 Aq. Phosphate Buffer 作为反应溶剂,化学反应 0.34H,反应生成 D-纤维二糖 参考文献:Cloning,Expression And Biochemical Characterization Of A Gh1 β-Glucosidase From Cellulosimicrobium Cellulans 标题:Cloning,Expression And Biochemical Characterization Of A Gh1 β-Glucosidase From Cellulosimicrobium Cellulans 摘要:Beta-Glucosidase Plays An Important Role In The Degradation Of Cellulose. In This Study,A Novel-Glucosidase Ccbgl1B Gene For A Glycosyl Hydrolase (Gh) Family 1 Enzyme Was Cloned From The Genome Of Cellulosimicrobium Cellulans And Expressed In Escherichia Coli Bl21 Cells. The Sequence Contained An Open Reading Frame Of 1494Bp,Encoded A Polypeptide Of 497Amino Acid Residues. The Recombinant Protein Ccbgl1B Was Purified By Ni Sepharose Fastflow Affinity Chromatography And Had A Molecular Weight Of 57Kda,As Judged By Sds-Page. The Optimum-Glucosidase Activity Was Observed At 55 Degrees C And Ph 6.0. Recombinant Ccbgl1B Was Found To Be Most Active Against Aryl-Glycosides P-Nitrophenyl--D-Glucopyranoside (Pnpglc),Followed By P-Nitrophenyl--D-Galactopyranoside (Pnpgal). Using Disaccharides As Substrates,The Enzyme Efficiently Cleaved-Linked Glucosyl-Disaccharides,Including Sophorose (-1,2-),Laminaribiose (-1,3-) And Cellobiose (-1,4-). In Addition,A Range Of Cello-Oligosaccharides Including Cellotriose,Cellotetraose And Cellopentaose Were Hydrolysed By Ccbgl1B To Produce Glucose. The Interaction Mode Between The Enzyme And The Substrates Driving The Reaction Was Modelled Using A Molecular Docking Approach. Understanding How The Gh1 Enzyme Ccbgl1B From C. Cellulans Works,Particularly Its Activity Against Cello-Oligosaccharides,Would Be Potentially Useful For Biotechnological Applications Of Cellulose Degradation. DOI:10.1080/10242422.2017.1395415
专利号:US-11473116-B2 优先权日:2018-02-23 标题 :Synthesis of glucan comprising alpha-1,3 glycosidic linkages with phosphorylase enzymes 发明人:YU ZHEYONG; KRALJ SLAVKO; ZHANG ZHENGHONG; HOWE LAURIE A 权利人:NUTRITION & BIOSCIENCES USA 4 INC 摘要:Reaction compositions are disclosed herein comprising at least water, beta-glucose -1-phosphate (beta-G1P), an acceptor molecule, and an alpha-1,3-glucan phosphorylase enzyme. These reactions can synthesize oligosaccharides and polysaccharides with alpha-1,3 glycosidic linkages. Further disclosed are alpha-1,3-glucan phosphorylase enzymes and methods of use thereof.
专利号:US-8846759-B2 优先权日:2009-12-24 标题 :Low-caloric high-protein nutritional composition for the stimulation of muscle protein synthesis 发明人:LUIKING YVETTE CHARLOTTE; VERLAAN GEORGE; JOURDAN MARION 权利人:LUIKING YVETTE CHARLOTTE; VERLAAN GEORGE; JOURDAN MARION; NUTRICIA NV 摘要:The present invention relates to the use of a low-caloric high-protein nutritional composition for use in the prevention or treatment of a disease or condition in a mammal, which involves muscle decline, as well as to specific low-caloric high-protein nutritional compositions for stimulating muscle protein synthesis in a mammal. In particular, the invention relates to the use of a nutritional composition comprising per 100 kcal: (i) at least about 12 g of proteinaceous matter which comprises at least about 80 weight % of whey protein, relative to the total proteinaceous matter, and which comprises at least about 11 weight % of leucine, relative to the total proteinaceous matter, of which at least about 20 weight % is in a free form, relative to the total leucine, (ii) a source of fat and a source of digestible carbohydrates, for the prevention or treatment of a disease or condition which involves muscle decline in a mammal, especially an elderly mammal, wherein the nutritional composition is administered as 1 to 2 servings daily, each serving comprising between 80 and 200 kcal.
专利号:US-9339782-B1 优先权日:2004-11-18 标题 :Electrode array device having an adsorbed porous reaction layer 发明人:GINDILIS ANDREI 权利人:CUSTOMARRAY INC 摘要:There is disclosed an electrode array device having an adsorbed porous reaction layer for improved synthesis quality. The array comprises a plurality of electrodes on a substrate, wherein the electrodes are electronically connected to a computer control system. The array has an adsorbed porous reaction layer on the plurality of electrodes, wherein the adsorbed porous reaction layer comprises a chemical species having at least one hydroxyl group. In the preferred embodiment, the reaction layer is sucrose. A method for preparing an electrode array for improved synthesis quality is disclosed. The method comprises a cleaning method and a method of attachment of a reaction layer. The cleaning method comprises a plasma cleaning method and a chemical cleaning method. The reaction layer is attached after cleaning by exposing the microarray to a solution containing the chemical species having at least one hydroxyl group.
专利号:EP-2590521-B1 优先权日:2010-07-07 标 题:Nutritional composition for the stimulation of muscle protein synthesis
专利号:US-2015290223-A1 优先权日:2010-07-07 标题 :Nutritional Composition for the Stimulation of Muscle Protein Synthesis
专利号:US-2013203658-A1 优先权日:2009-12-24 标题 :Low-Caloric High-Protein Nutritional Composition for the Stimulation of Muscle Protein Synthesis
参考文献:10.1007/978-3-030-78444-7_39 摘要:Sánchez-Vega R, Chávez-Martínez A, Tirado-Gallegos JM, Reyes-Jurado F, Ochoa-Velasco CE, Avila-Sosa R. Opuntia spp. Products and By-products as a Potential Source of Edible Films and Coatings. 2021. In: Opuntia spp.: Chemistry, Bioactivity and Industrial Applications. 参考文献:10.1007/978-981-99-3435-5_7 摘要:Kaur D, Singh A, Dalal S, Sharma J. Rice Straw Waste Utilization for Nanoparticles Synthesis and Their Various Applications. 2023. In: Smart Nanomaterials Technology.