Methyl Sinapate置于3-吗啉丙磺酸,溶剂黄146体系中,化学反应 0.17H,反应生成 芥子酸 参考文献:Dependency Of The Hydrogen Bonding Capacity Of The Solvent Anion On The Thermal Stability Of Feruloyl Esterases In Ionic Liquid Systems 标题:Dependency Of The Hydrogen Bonding Capacity Of The Solvent Anion On The Thermal Stability Of Feruloyl Esterases In Ionic Liquid Systems 摘要:三种阿魏酸酯酶 Ec 3.1.1.73 (Fae),即来自黑曲霉的 Fae A (Anfaea),来自构巢曲霉的 Fae C (Andfaec) 以及来自特异腐质霉 (Ultraflo L) 的商业 β-葡聚糖酶混合物中的 Fae 活性测试了它们在四种离子液体(il)系统中催化芥子酸与甘油酯化的能力. Il 系统系统地由两对选定的阳离子和阴离子组成,分别为:[bmim][pf6],[c2Ohmim][pf6],[bmim][bf4] 和 [c2Ohmim][bf4]. Anfaea 在基于 [pf6] 的 Il 中具有活性,而 Ultraflo L 中的 Andfaec 和 Fae 没有明显的活性,并且在 Il 系统中通常不稳定. Il 系统中的 Fae 稳定性显然高度依赖于酶结构,值得注意的是 Anfaea 与 Il 相容性脂肪酶的相似性可以解释其稳定性. Anfaea 在缓冲液中的热稳定性高于 Il 系统,但在 40 oc 及以下,缓冲液和基于 [pf6]− 的系统之间 Anfaea 的稳定性没有显着差异:Anfaea置于[bmim] 中稳定][pf6] 和 [c2Ohmim][pf6] 系统在 40 oc 下反应 2 小时.然而,Il 阴离子对稳定性有重大影响:[bf4]− 导致 Anfaea 快速失活,而 [pf6]− 则不然.阳离子没有类似的效果.这些观察结果可以通过 Cosmo-Rs 模拟用 Il 阳离子和阴离子的氢键能力来解释. DOI:10.1039/c1Gc15115K
专利号:US-2012190064-A1 优先权日:2004-10-01 标题 :Feeding buffers, systems, and methods for in vitro synthesis of biomolecules 发明人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA 权利人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA; LIFE TECHNOLOGIES CORP 摘要:Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of a feeding solution that includes amino acids and an energy source to an ongoing in vitro synthesis system. The invention also includes methods of using a high-yield in vitro synthesis system to produce large quantities of proteins with incorporated labeled amino acids for analysis by methods such as by NMR. The invention further includes vectors for enhanced production of proteins from nucleic acid templates using in vitro synthesis systems.
专利号:US-2024024489-A1 优先权日:2020-11-20 标 题:Protected disaccharides, their process of preparation and their use in the synthesis of zwitterionic oligosaccharides, and conjugates thereof 发明人:MULARD LAURENCE; DHARA DEBASHIS; PFISTER HELENE; PAOLETTI JULIE; PHALIPON ARMELLE; GUERREIRO-INVERNO CATHERINE 权利人:PASTEUR INSTITUT 摘要:The present invention provides zwitterionic oligosaccharides, in particular fragments of the surface polysaccharides from Shigella sonnei and Shigella sonnei conjugates comprising them. The present invention also provides protected disaccharides, their process of preparation and their use in the synthesis of zwitterionic oligosaccharides, and conjugates thereof, the disaccharide repeating unit of Shigella sonnei being: (I)
专利号:US-2023119068-A1 优先权日:2020-02-13 标题:Methods for rapid synthesis of pyranoanthocyanins 发明人:ZHU XIAOYI; STRAATHOF NICOLE; MIYAGUSUKU-CRUZADO GONZALO; GIUSTI M MONICA; CANO ISRAEL GARCIA; JIMENEZ-FLORES RAFAEL 权利人:OHIO STATE INNOVATION FOUNDATION 摘要:The present disclosure provides methods for the rapid synthesis of pyranoanthocyanins.
专利号:WO-2009033269-A1 优先权日:2007-09-11 标 题 :Novel polysaccharide immunogens from alloiococcus otitidis and synthesis of a glycoconjugate vaccine thereof 发明人:MONTEIRO MARIO ARTUR; ARAR SARIF; HARIMAYA ATSUSHI 权利人:UNIV GUELPH; MONTEIRO MARIO ARTUR; ARAR SARIF; HARIMAYA ATSUSHI 摘要:The application relates to Alloiococcus otitidis cell surface polysaccharides, compositions comprising Alloiococcus otitidis cell surface polysaccharides, synthesis of a glycoconjugate vaccine and includes kits, methods and uses thereof.
专利号:US-2025250302-A1 优先权日:2024-02-05 标题:Synthesis of self-assembling artificial proteins utilizing a host-guest system and uses thereof 发明人:SANDANARAJ BRITTO S; KHYADE ASHWINI RAJENDRA; HATI KSHITISH CHANDRA 权利人:INDIAN INSTITUTE OF SCIENCE EDUCATION AND RES PUNE IISER PUNE 摘要:The invention relates to an efficient method for synthesizing self-assembling artificial proteins (SAPs) utilizing a catalytic host-guest system. The process involves encapsulating hydrophobic probes with cyclodextrin, performing site-specific protein bioconjugation, and employing a second catalysis cycle for enhanced labeling. SAPs produced through this method are versatile and find applications in therapeutic delivery and diagnostics. The process is simplified, scalable, cost-effective, and environmentally sustainable, addressing the challenges of traditional SAP synthesis. By offering improved yield, functionality, and structural integrity, this invention advances the potential of SAPs in medical and industrial fields.
专利号:US-8647848-B2 优先权日:2008-06-13 标题:Enzymatic synthesis of sphingolipids 发明人:HOLLMANN FRANK; THUM OLIVER; TOELLE CHRISTOPH; PROVINZANO ANGELO; KOREVAAR CORNELIS GERRIT NIJS 权利人:HOLLMANN FRANK; THUM OLIVER; TOELLE CHRISTOPH; PROVINZANO ANGELO; KOREVAAR CORNELIS GERRIT NIJS; EVONIK GOLDSCHMIDT GMBH 摘要:The invention relates to the enzymatic synthesis of sphingolipids and compositions that contain sphingolipids from lysosphingolipids and carbonic esters, and to cosmetic, dermatological or pharmaceutical formulations containing said sphingolipids or compositions.
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合成参考文献
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