专利号:US-5374541-A 优先权日:1993-05-04 标 题 :Combined use of β-galactosidase and sialyltransferase coupled with in situ regeneration of CMP-sialic acid for one pot synthesis of oligosaccharides 发明人:WONG CHI-HUEY; GAETA FEDERICO C A 权利人:SCRIPPS RESEARCH INST; CYTEL CORP 摘要:A single reaction vessel process for the synthesis of a sialylated galactoside is disclosed. The synthesis utilizes a β-galactosidase to catalyze the reaction of a galactose-containing substrate and an acceptor to form a new galactosyl glycoside that is then sialylated using a cyclic multienzyme synthesis system to form CMP-sialic acid that sialylates the formed galactosyl glycoside in the presence of an α-sialyltransferase. The value of K m /V max for the formed galactosyl glycoside as a substrate for the α-sialyltransferase is less than one-third the K m /V max value for the galactose-containing substrate for that α-sialyltransferase.
专利号:US-9217012-B2 优先权日:2009-04-08 标题 :Inhibitors of protein tyrosine phosphatases 发明人:ZHANG ZHONG-YIN; ZHANG SHENG 权利人:ZHANG ZHONG-YIN; ZHANG SHENG; UNIV INDIANA RES & TECH CORP 摘要:Disclosed herein are compounds that selectively inhibit members of the PTP family of enzymes. Synthesized compounds demonstrated selective inhibition of TC-PTP. Also provided are methods of using the compounds and formulations containing the compounds. Also described is a fluorescence-tagged combinatorial library synthesis and screening method. And methods of using these compounds to effect enzyme activity both in cells and in vitro as well as method of using these compounds to treat diseases in human and animals.
专利号:JP-3759603-B2 优先权日:1993-05-04 标题 :One-pot synthesis of oligosaccharides using multienzymes and CMP-sialic acid regeneration system
专利号:EP-0698112-B1 优先权日:1993-05-04 标 题 :One pot synthesis of oligosaccharides using multiple enzymes and cmp-sialic acid regenerating system
专利号:US-5574146-A 优先权日:1994-08-30 标题:Oligonucleotide synthesis with substituted aryl carboxylic acids as activators 发明人:REDDY M PARAMESWARA; FAROOQUI FIRDOUS 权利人:BECKMAN INSTRUMENTS INC 摘要:A process for synthesizing oligonucleotides by phosphoramidite chemistry wherein the improvement is the use of substituted aryl carboxylic acids as the activators. These activators produce in situ nucleotide intermediates in which the substituted arylcarbonyl group has displaced the amidite moiety.
专利号:NZ-538522-A 优先权日:2002-08-23 标 题 :Synthesis of epothilones, intermediates thereto, analogues and uses thereof
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