2,6,8-三氯嘌呤置于phosphonium Iodide,水,氢碘酸体系中,化学反应生成 6-糠氨基嘌呤 参考文献:Purines. Viii. The Aminolysis Of Certain Chlorosubstituted Purines1 标题:Purines. Viii. The Aminolysis Of Certain Chlorosubstituted Purines1 摘要: DOI:10.1021/ja01523A075
专利号:US-2024110196-A1 优先权日:2022-08-29 标题 :Use of multigene stacking method in synthesis of nervonic acid in brassica napus 发明人:LIU FANG; WANG PANDI; WU GANG; XIONG XIAOJUAN 权利人:OIL CROPS RES INSTITUTE CAAS 摘要:The present disclosure relates to use of a multigene stacking method in synthesis of nervonic acid in Brassica napus. The present disclosure provides a multigene co-expression plant vector, including an initial backbone of pBWA(V)BII and multiple exogenous gene expression cassettes. The present disclosure further provides two plant vectors applicable to genetic transformation, including a three-gene co-expression plant vector and a five-gene co-expression plant vector. In the present disclosure, after the three-gene co-expression plant vector and the five-gene co-expression plant vector are separately transferred into the Brassica napus, the nervonic acid (NA) with a high content is synthesized in the Brassica napus through multigene co-expression. An NA ratio can be significantly increased combined with a higher seed oil content and a greater field seed yield. The plant vector realizes efficient synthesis of NA in oil crops, obtains NA-rich seed oil, and increases an added value of edible oil.
专利号:US-4101721-A 优先权日:1976-12-09 标题 :Solid phase synthesis of protected peptides 发明人:RICH DANIEL H; GURWARA SWEET K 权利人:WISCONSIN ALUMNI RES FOUND 摘要:The invention is addressed to the preparation of 3-nitro-4-bromomethyl benzoic acid, as a new compound from which 3-nitro-4-bromomethyl benzoyl amide polystyrene resin can be prepared for solid synthesis of protected peptide acids and amides and separation thereof without cleavage of acid labile protecting groups or decomposition of aromatic acid groups and from which purified polypeptides can be formed.
专利号:US-4062746-A 优先权日:1975-05-07 标 题 :Solid phase synthesis of protected peptides 发明人:RICH DANIEL H; GURWARA SWEET K 权利人:WISCONSIN ALUMNI RES FOUND 摘要:The invention is addressed to the preparation of 3-nitro-4-bromomethyl benzoic acid, as a new compound from which 3-nitro-4-bromomethyl benzoyl amide polystyrene resin can be prepared for solid synthesis of protected peptide acids and amides and separation thereof without cleavage of acid labile protecting groups or decomposition of aromatic acid groups and from which purified polypeptides can be formed.
专利号:US-5344775-A 优先权日:1991-11-15 标题 :Synthesis of taxanes in culture using pseudocalluscells 发明人:SMITH RICHARD J 权利人:ESCAGENETICS CORP 摘要:A method of synthesis of taxanes from pseudocallus of Taxus species, particularly T. baccata , and methods for pseudocallus production are described. Pseudocallus of the invention can be distinguished from callus tissue and cell suspensions by (1) its unique morphology which is characterized by an amorphous aggregation of cells lacking both differentiated tissues and clearly defined meristematic zones; by (2) very poor intercellular adhesion resulting in marked tissue friability; and by (3) relatively high initial rate of mass doubling. Production of taxanes is achieved by culturing the pseudocallus on support culture medium.
专利号:US-4849513-A 优先权日:1983-12-20 标 题 :Deoxyribonucleoside phosphoramidites in which an aliphatic amino group is attached to the sugar ring and their use for the preparation of oligonucleotides containing aliphatic amino groups 发明人:SMITH LLOYD M; FUNG STEVEN; KAISER ROBERT J 权利人:CALIFORNIA INST OF TECHN 摘要:The invention consists of compounds and methods for the synthesis of oligonucleotides which contain one or more free aliphatic amino groups attached to the sugar moieties of the nucleoside subunits. The synthetic method is versatile and general, permitting amino groups to be selectively placed at any position on oligonucleotides of any composition or length which is attainable by current DNA synthetic methods. Fluorescent dyes or other detectable moieties may be covalently attached to the amino groups to yield the corresponding modified oligonucleotide.
专利号:US-5118802-A 优先权日:1983-12-20 标 题:DNA-reporter conjugates linked via the 2' or 5'-primary amino group of the 5'-terminal nucleoside 发明人:SMITH LLOYD M; FUNG STEVEN; KAISER JR ROBERT J 权利人:CALIFORNIA INST OF TECHN 摘要:The invention consists of compounds and methods for the synthesis of oligonucleotides which contain one or more free aliphatic amino groups attached to the sugar moieties of the nucleoside subunits. The synthetic method is versatile and general, permitting amino groups to be selectively placed at any position on oligonucleotides of any composition or length which is attainable by current DNA synthetic methods. Fluorescent dyes or other detectable moieties may be covalently attached to the amino groups to yield the corresponding modified oligonucleotide.
1: Kaźmierczak A, Soboska K. Fate of nuclear material during subsequent steps of the kinetin-induced PCD in apical parts of Vicia faba ssp. minor seedling roots. Micron. 2018 Apr 30;110:79-87. doi: 10.1016/j.micron.2018.04.009. [Epub ahead of print] doi: 10.4103/pm.pm_170_17. Epub 2018 Apr 10. 3: Li Q, Wang H, Wang H, Zheng W, Wu D, Wang Z. Effects of kinetin on plant growth and chloroplast ultrastructure of two Pteris species under arsenate stress. Ecotoxicol Environ Saf. 2018 Apr 12;158:37-43. doi: 10.1016/j.ecoenv.2018.04.009. [Epub ahead of print] doi: 10.1007/s13205-018-1235-x. Epub 2018 Apr 9. 5: Zhang L, Hoshika Y, Carrari E, Burkey KO, Paoletti E. Protecting the photosynthetic performance of snap bean under free air ozone exposure. J Environ Sci (China). 2018 Apr;66:31-40. doi: 10.1016/j.jes.2017.05.009. Epub 2017 May 14. doi: 10.1016/j.ejmech.2018.03.043. Epub 2018 Mar 21. doi: 10.1007/s13205-018-1206-2. Epub 2018 Mar 14.
合成参考文献
参考文献:10.1055/s-2005-873136 摘要:Rojas G, Aranda E, Navarro V, Zamilpa A, Tortoriello J. In vitro propagation of Galphimia glauca and content of the sedative compound galphimine-B in wild and micropropagated plants. Planta Med. 2005 Nov;71(11):1076–8. doi: 10.1055/s-2005-873136. 参考文献:10.1007/978-1-61779-182-6_15 摘要:Ayaydin F, Kotogány E, Abrahám E, Horváth GV. Synchronization of Medicago sativa cell suspension culture. Methods Mol Biol. 2011;761():227–38. doi: 10.1007/978-1-61779-182-6_15. 参考文献:10.1094/mpmi-05-11-0142 摘要:Heckmann AB, Sandal N, Bek AS, Madsen LH, Jurkiewicz A, Nielsen MW, Tirichine L, Stougaard J. Cytokinin induction of root nodule primordia in Lotus japonicus is regulated by a mechanism operating in the root cortex. Mol Plant Microbe Interact. 2011 Nov;24(11):1385–95. doi: 10.1094/mpmi-05-11-0142. 参考文献:10.1155/2011/165852 摘要:Haghbeen K, Pourmolaei S, Mareftjo MJ, Mousavi A, Akbari Noghabi K, Hosseini Shirazi F, Meshkat A. Detailed Investigations on the Solid Cell Culture and Antimicrobial Activities of the Iranian Arnebia euchroma. BioMed Research International. 2011 Jan;2011(1). doi: 10.1155/2011/165852. 参考文献:10.1203/pdr.0b013e31822e1825 摘要:Axelrod FB, Liebes L, Gold-von Simson G, Mendoza S, Mull J, Leyne M, Norcliffe-Kaufmann L, Kaufmann H, Slaugenhaupt SA. Kinetin Improves IKBKAP mRNA Splicing in Patients With Familial Dysautonomia. Pediatr Res. 2011 Nov;70(5):480–3. doi: 10.1203/pdr.0b013e31822e1825.