专利号:WO-8002033-A1 优先权日:1979-03-27 标 题 :Immobilized enzyme/cell system for the production of alpha-keto acids 发明人:MOSBACH K; BRODELIUS P 权利人:MOSBACH K; BRODELIUS P 摘要:(Alpha)-keto acids are prepared from the corresponding amino acids by using a biocatalyst of a microbial origin, which has been immobilized in a polymeric matrix. The immobilized biocatalyst is effective and specific for the synthesis of (Alpha)-keto acids from D-amino acids. The product can easily be separated from the particulate catalyst. In the synthesis reaction, hydrogen peroxide is formed, which can be decomposed by a catalyst which also can be immobilized in the polymeric matrix. The invention can be used in continuous or discontinuous processes for the preparation of (Alpha)-keto acids from the corresponding D, L- or D amino acids.
专利号:US-2015001155-A1 优先权日:2013-06-26 标 题:Methods and apparatus for synthesis of stabilized zero valent nanoparticles 发明人:JOHNSON BENEDICT YORKE 权利人:CORNING INC 摘要:Methods and apparatus provide for zero valent nanoparticles coated with a stabilizer to inhibit oxidation, where the coating includes at least one of activated carbon, graphene, an inorganic oxide, and an organic material.
专利号:US-5591838-A 优先权日:1993-02-12 标 题:Detection of conversion to mucoidy in pseudomonas aeruginosa infecting cystic fibrosis patients 发明人:DERETIC VOJO; MARTIN DANIEL W 权利人:UNIV TEXAS 摘要:Compositions and methods for detecting the conversion to mucoidy in Pseudomonas aeruginosa are disclosed. Chronic respiratory infections with mucoid Pseudomonas aeruginosa are the leading cause of high mortality and morbidity in cystic fibrosis. The initially colonizing strains are nonmucoid but in the cystic fibrosis lung they invariably convert into the mucoid form causing further disease deterioration and poor prognosis. The molecular basis of this conversion to mucoidy is also disclosed. The algU gene encodes a protein homologous to an alternative sigma factor regulating sporulation and other developmental processes in Bacillus, and along with the negative regulators mucA and mucB comprises the gene cluster controlling conversion to mucoidy. The switch from nonmucoid to mucoid state is caused by frameshift deletions and duplications in the second gene of the cluster, mucA. Inactivation of mucA results in constitutive expression of genes, such as algD, dependent on algU for transcription. Insertional inactivation of mucB on the chromosome of the standard genetic strain PAO also resulted in mucoid phenotype, and in a strong transcriptional activation of algD. Activation of algD results in increased synthesis of the exopolysaccharide alginate rendering P. aeruginosaa mucoid.
专利号:US-2010151526-A1 优先权日:2005-09-22 标 题 :Method of Producing Sucrose-6-Acetate by Whole-Cell Biocatalysis 发明人:RATNAM RAKESH; AURORA SUNDEEP; SUBRAMANIYAM P 权利人:PHARMED MEDICARE PVT LTD 摘要:A process is described which uses whole cell preparations, immobilized or without immobilization, of a microorganism, including Aureobasidium pullulans , capable of forming enzymes of the group of fructosyltransferases, for catalyzing a reaction between sucrose and 6-O-protected glucose for formation of 6-O-protected sucrose, an intermediate in synthesis of trichlorogalactosucrose. 6-O-protected Sucrose is separated from high molecular weight by-products of the reaction having molecular weight of 500 daltons and more by reverse osmosis, and further purified by column chromatography.
专利号:US-4562151-A 优先权日:1983-09-06 标 题:Stabilization of L-phenylalanine ammonia-lyase enzyme 发明人:KISHORE GANESH M 权利人:MONSANTO CO 摘要:A process for the synthesis of L-phenylalanines and analogues thereof from trans-cinnamate, ring substituted trans-cinnamates or various acrylate derivatives and ammonia or an ammonia donor in the presence of L-phenylalanine ammonia-lyase enzyme and a desensitizing agent such as polyhydric alcohols and polyethylene glycol-(400) is disclosed. The process embraces the discovery that compounds such as polyhydric alcohols and polyethylene glycol-(400) desensitize the L-phenyalanine ammonia-lyase enzyme to substantially higher substrate concentrations than practiced heretofore. In addition, polyhydric alcohols and polyethylene glycol-(400) substantially enhance the instantaneous rate of reaction and inhibit inactivation of the L-phenylalanine ammonia lyase enzyme over longer reaction periods.
专利号:US-10569253-B1 优先权日:2017-08-21 标 题:Synthesis of biocomposite alginate-chitosan-magnetite nanoparticle beads for removal of organic persistent contaminants from water systems 发明人:ROMAN VELAZQUEZ FELIX; FERNANDEZ ALOS VICTOR; PERALES PEREZ OSCAR 权利人:ROMAN VELAZQUEZ FELIX; FERNANDEZ ALOS VICTOR; PERALES PEREZ OSCAR; UNIV PUERTO RICO 摘要:The invention provides biocomposites alginate/chitosan beads integrated with magnetite nanoparticles and modified-surface magnetite derivate created and designed to remove from environmental waters and aquatic systems different types of organic persistent compounds such as benzophenone-3 (oxybenzone).
参考文献:10.1016/j.ijbiomac.2014.05.056 摘要:Cong Q, Xiao F, Liao W, Dong Q, Ding K. Structure and biological activities of an alginate from Sargassum fusiforme, and its sulfated derivative. International Journal of Biological Macromolecules. 2014 Aug;69():252–9. doi: 10.1016/j.ijbiomac.2014.05.056.