专利号:US-2023212216-A1 优先权日:2019-12-20 标 题:Synthesis of lactone derivatives and their use in the modification of proteins 发明人:MORRIS ALEXANDER REDFERN; SUTOV GRIGORIJ; BRUNE KARL DIETRICH 权利人:GENIE BIOTECH UK LTD 摘要:Site-specific modifications of proteins are desirable in biotechnological applications such as biopharmaceuticals, immunotherapy, vaccines, and are useful in chemical biology. Gluconoylation is a non-enzymatic, covalent, post-translational modification commonly observed on N-terminal His-Tags bearing proteins. We synthesized glucono-1,5-lactone derivatives, including azido variants for selective acylation. High yield acylation is achieved by simply mixing derivatives with target protein amidst diverse conditions of temperatures, aqueous buffers, excipients, or complex cell lysate.
专利号:WO-2025037347-A1 优先权日:2023-08-16 标 题:Formulation for improving sweetness of the rare sugars without altering the texture and flavour and to the method thereof 发明人:KANUMURU RAHUL RAJU 权利人:FERTIS INDIA PVT LTD 摘要:Disclosed herein is a formulation comprising sweet protein with rare sugars for improving the sweetness of the rare sugars with low glycemic index without changing the texture and flavours of said rare sugars and to the method for improving the sweetness of the rare sugars. More particularly, the present invention discloses method for improving the sweetness of the rare sugars without changing the sweetness profile, texture and flavours of the primary sugars. The present invention further discloses addition of Brazzein with Liquid Rare sugars before crystallization or at pre caramalization stage or post caramalization stage of rare sugars at different temperatures during the synthesis of rare sugars to increase the sweetness. Brazzein increases the sweetness of Rare sugars but not limited to D-Allulose, D-tagatose, D-Allose, Isomaltulose, Trehalulose, Sorbose, turanose, Leucrose, etc. The present invention further provides evaluation of non-nutritive sweeteners such as aspartame, acesulfame-K, Cyclamate, Neotame, Saccharin, Sucralose, Stevia etc., as each one has limitations for taste quality if used with Rare sugars. However, the use of Brazzein did not change the flavour and texture of the rare sugars and the taste profile matches the sucrose.
专利号:US-2025282806-A1 优先权日:2024-05-14 标 题:Chemical synthesis method for disaccharide fragment of vibrio vulnificus biotype 2 serovar a o-antigen 发明人:YIN JIAN; HU JING; ZHU SHENGYONG; QIN CHUNJUN 权利人:UNIV JIANGNAN 摘要:The disclosure provides a chemical synthesis method for a disaccharide fragment of a Vibrio vulnificus biotype 2 serovar A O-antigen, belonging to the chemical field. The disclosure utilizes D-glucose and L-galactose as raw materials to prepare two types of glycosylated building blocks, and designs an efficient synthesis route for constructing the disaccharide fragment. By optimizing protecting groups and optimizing the time of introducing modifying groups, the preparation of the target disaccharide is successfully completed. The raw materials for preparing the disaccharide in the disclosure are readily available, the preparation method is simple and easy to repeat, and will have a good application prospect in the development of new drugs and vaccines for V. vulnificus.
专利号:WO-02103001-A1 优先权日:2001-06-15 标 题 :Gdp-mannose-3',5'-epimerase and methods of use thereof 发明人:WOLUCKA BEATA 权利人:VLAAMS INTERUNIV INST BIOTECH; WOLUCKA BEATA 摘要:The identification of a new key enzyme in vitamin C synthesis, and its use to modulate vitamin C synthesis in eukaryotic cells are disclosed. Specifically, the isolation of a multimeric GDP-mannose-3',5'-epimerase, the identification, cloning and expression of a nucleic acid sequence encoding GDP-mannose-3',5'-epimerase, methods of producing GDP-mannose-3',5'-epimerase, transgenic plants and microorganisms that express the GDP-mannose-3',5'-epimerase, and methods of production of ascorbic acid using the GDP-mannose-3',5'-epimerase are disclosed.
专利号:US-5078796-A 优先权日:1989-07-19 标 题:Process for manufacturing tagatose 发明人:BEADLE JAMES R; SAUNDERS JAMES P; WAJDA JR THOMAS J 权利人:BIOSPHERICS INC 摘要:A process is disclosed for the synthesis of D-tagatose by isomerizing a mixture containing D-galactose with a metal hydroxide in the presence of a catalyst at a relatively low temperature to form an intermediate metal hydroxide-D-tagatose complex, and then neutralizing the intermediate with acid to yield D-tagatose. The method is also suitable for the synthesis of L-tagatose from L-galactose, and for the recovery of pure tagatose from crude tagatose syrups. Whey, deproteinized whey, or lactose may be used as the raw material for the D-galactose. The lactose in such cases is hydrolyzed to D-galactose and D-glucose before the isomerization step.
专利号:US-5002612-A 优先权日:1989-07-19 标题 :Process for manufacturing tagatose 发明人:BEADLE JAMES R; SAUNDERS JAMES P; WAJDA JR THOMAS J 权利人:BIOSPHERICS INC 摘要:A process is disclosed for the synthesis of D-tagatose by isomerizing a mixture containing D-galactose with a metal hydroxide in the presence of a catalyst at a relatively low temperature to form an intermediate metal hydroxide-D-tagatose complex, and then neutralizing the intermediate with acid to yield D-tagatose. The method is also suitable for the synthesis of L-tagatose from L-galactose, and for the recovery of pure tagatose from crude tagatose syrups. Whey, deproteinized whey, or lactose may be used as the raw material for the D-galactose. The lactose in such cases is hydrolyzed to D-galactose and D-glucose before the isomerization step.
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