专利号:US-2012190064-A1 优先权日:2004-10-01 标题 :Feeding buffers, systems, and methods for in vitro synthesis of biomolecules 发明人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA 权利人:KUDLICKI WIESLAW ANTONI; KEPPETIPOLA SHIRANTHI; FLETCHER JULIA; GETBEHEAD ASHLEY ELAINE; KATZEN FEDERICO; VOZZA-BROWN LAURA; LIFE TECHNOLOGIES CORP 摘要:Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of a feeding solution that includes amino acids and an energy source to an ongoing in vitro synthesis system. The invention also includes methods of using a high-yield in vitro synthesis system to produce large quantities of proteins with incorporated labeled amino acids for analysis by methods such as by NMR. The invention further includes vectors for enhanced production of proteins from nucleic acid templates using in vitro synthesis systems.
专利号:US-6506902-B2 优先权日:2000-03-29 标题:Synthesis of thiazolium compounds 发明人:WAGLE DILIP 权利人:ALTEON INC 摘要:Provided is a method of synthesizing a compound of formula I, n comprising: n (a) reacting a compound of formula II n wherein R 1 and R 2 are independently hydrogen, hydroxy(C 1 -C 2 )alkyl, or (C 1 -C 2 )alkyl with, n a compound of formula III n wherein n R 3 , R 4 , and R 5 are each independently of each other hydrogen, (C 1 -C 3 )alkyl, (C 1 -C 3 )alkoxy, or halogen; and n X is a leaving group, n in a solvent having a dielectric constant at 20° C. of at least 30 but no more than 40; and n (b) obtaining the compound I.
专利号:US-2002013471-A1 优先权日:2000-03-29 标 题:Synthesis of thiazolium compounds
专利号:US-2009209032-A1 优先权日:2004-10-01 标 题 :Feeding buffers, systems, and methods for in vitro synthesis of biomolecules
专利号:EP-1848812-A2 优先权日:2004-10-01 标题:Feeding buffers, systems, and methods for in vitro synthesis of biomolecules
专利号:US-2006110788-A1 优先权日:2004-10-01 标题 :Feeding buffers, systems, and methods for in vitro synthesis of biomolecules
[参考文献]: W S Hancock, Et Al. Rapid Analysis Of 3-Methylhistidine In Urine, Plasma, Muscle And Amniotic Fluid With A Single High-Performance Liquid Chromatographic System But With Different Ion-Pairing Reagents. J Chromatogr. 1982 Mar 12:228:273-8.
合成参考文献
摘要:Lai, J.; Thomson, B. J.; Sammis, G. M., Science of Synthesis: Modern Strategies in Organofluorine Chemistry , (2024) 1, 2.